Restriction map for pF1KB8456
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BanII       BbeI        BbvCI       BcgI        BciVI       
BcnI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsmI        BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HaeII       
HincII      HindII      HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PinAI       
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfaNI       SfiI        SfoI        SfuI        SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        TaqI        TthHB8I     XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccII         992  CGCG                 CG^CG
AcyI            3  GGCGTC               G[GA]^CG[CT]C
Alw44I        922  GTGCAC               G^TGCAC
ApaLI         922  GTGCAC               G^TGCAC
AspHI         922  GTGCAC               G[AT]GC[AT]^C
AspI          506  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          946  CCCGAG               C^[CT]CG[GA]G
AvaII        1194  GGTCC                G^G[AT]CC
BalI         1421  TGGCCA               TGG^CCA
BamHI         843  GGATCC               G^GATCC
BbrPI         925  CACGTG               CAC^GTG
BbsI          301  GAAGAC               GAAGAC(2/6)
BclI         1091  TGATCA               T^GATCA
BglI         1408  GCCATCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         301  GAAGAC               GAAGAC(2/6)
BsaAI         925  CACGTG               [CT]AC^GT[GA]
BsaHI           3  GGCGTC               G[GA]^CG[CT]C
BsaI          424  GAGACC               GGTCTC(1/5)
BsiHKAI       922  GTGCAC               G[AT]GC[AT]^C
BsoBI         946  CCCGAG               C^[CT]CG[GA]G
Bsp1407I      415  TGTACA               T^GTACA
BsrFI        1336  GCCGGT               [GA]^CCGG[CT]
BsrGI         415  TGTACA               T^GTACA
BstUI         992  CGCG                 CG^CG
BstYI         843  GGATCC               [GA]^GATC[CT]
Cfr10I       1336  GCCGGT               [GA]^CCGG[CT]
DraII         813  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DrdI          698  GACAAGTCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         1421  TGGCCA               [CT]^GGCC[GA]
EcoO109I      813  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       117  CCAAGG               C^C[AT][AT]GG
FbaI         1091  TGATCA               T^GATCA
HapII        1337  CCGG                 C^CGG
HgaI            4  GCGTC                GACGC(5/10)
Hin1I           3  GGCGTC               G[GA]^CG[CT]C
HindIII       861  AAGCTT               A^AGCTT
HpaII        1337  CCGG                 C^CGG
MflI          843  GGATCC               [GA]^GATC[CT]
MluNI        1421  TGGCCA               TGG^CCA
MscI         1421  TGGCCA               TGG^CCA
MslI         1083  CACAGAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI         1337  CCGG                 C^CGG
MvnI          992  CGCG                 CG^CG
NheI          772  GCTAGC               G^CTAGC
PflFI         506  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         925  CACGTG               CAC^GTG
PmlI          925  CACGTG               CAC^GTG
SgrAI        1335  CGCCGGTG             C[GA]^CCGG[CT]G
SspBI         415  TGTACA               T^GTACA
StyI          117  CCAAGG               C^C[AT][AT]GG
TfiI          644  GAATC                G^A[AT]TC
Tth111I       506  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1321  TCTAGA               T^CTAGA
XhoII         843  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI          161  AAATTT               [GA]^AATT[CT]
              218  AAATTT              
AfaI          416  GTAC                 GT^AC
              576  GTAC                
AlwNI         337  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
              441  CAGCGGCTG           
ApoI          161  AAATTT               [GA]^AATT[CT]
              218  AAATTT              
BanI          462  GGTGCC               G^G[CT][GA]CC
              942  GGTGCC              
BsmAI         424  GAGAC                GTCTC(1/5)
              717  GTCTC               
BstF5I        675  CATCC                GGATG(2/0)
             1410  CATCC               
CfoI          527  GCGC                 GCG^C
              991  GCGC                
FokI          675  CATCC                GGATG(9/13)
             1410  CATCC               
HhaI          527  GCGC                 GCG^C
              991  GCGC                
HinP1I        527  GCGC                 G^CGC
              991  GCGC                
MaeII         507  ACGT                 A^CGT
              926  ACGT                
MseI          169  TTAA                 T^TAA
              176  TTAA                
MspA1I        441  CAGCGG               C[AC]G^C[GT]G
             1357  CCGCTG              
PflMI         440  CCAGCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              731  CCACTTCCTGG         
PstI          535  CTGCAG               CTGCA^G
              650  CTGCAG              
RsaI          416  GTAC                 GT^AC
              576  GTAC                
SapI          193  GAAGAGC              GCTCTTC(1/4)
             1267  GAAGAGC             
SfcI          535  CTGCAG               C^T[GA][CT]AG
              650  CTGCAG              
SmlI           80  CTCAAG               C^T[CT][GA]AG
              616  CTTGAG              
Tru9I         169  TTAA                 T^TAA
              176  TTAA                
Van91I        440  CCAGCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              731  CCACTTCCTGG         
XcmI         1052  CCACCTCCCTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1409  CCATCCTGGCAGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BfaI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        BsmFI       Bsp1286I    BsrDI       BsrI        
BssKI       BstNI       Cac8I       Cfr13I      DdeI        
DpnII       EarI        EcoRII      FauI        Fnu4HI      
HaeIII      HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MnlI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        Sau3AI      Sau96I      ScrFI       TseI        
Tsp45I      Tsp509I     TspRI       
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