Restriction map for pF1KB8455
##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
AlwI        AocI        Aor51HI     ApaI        ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AvaI        AvrII       BalI        BamHI       
BanI        BbeI        BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsiEI       BsiWI       BsmAI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrDI       BsrGI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstNI       BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DdeI        DraI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT14I     
EcoT22I     FbaI        FokI        FseI        HgaI        
Hin1I       HincII      HindII      HindIII     HinfI       
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeIII      MboII       MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MunI        MvaI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PinAI       PleI        PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        TfiI        Tru9I       Tsp45I      
Tsp509I     TspRI       TthHB8I     XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccI          283  GTCTAC               GT^[AC][GT]AC
AfaI          108  GTAC                 GT^AC
Alw44I        118  GTGCAC               G^TGCAC
AlwNI          73  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         118  GTGCAC               G^TGCAC
AspI          277  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         263  TGCGCA               TGC^GCA
BanII          64  GAGCTC               G[GA]GC[CT]^C
BbrPI         121  CACGTG               CAC^GTG
BpmI          156  CTCCAG               CTGGAG(16/14)
BsaAI         121  CACGTG               [CT]AC^GT[GA]
BsaBI           6  GATGTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          184  CTGCAC               GTGCAG(16/14)
BsmFI         127  GTCCC                GGGAC(10/14)
BsrBI         219  CCGCTC               CCGCTC(-3/-3)
BsrFI          91  ACCGGC               [GA]^CCGG[CT]
BsrI          105  CCAGT                ACTGG(1/-1)
Cfr10I         91  ACCGGC               [GA]^CCGG[CT]
DpnII         201  GATC                 ^GATC
FauI           32  CCCGC                CCCGC(4/6)
FspI          263  TGCGCA               TGC^GCA
HaeII         257  AGCGCC               [GA]GCGC^[CT]
HphI           68  TCACC                GGTGA(8/7)
MamI            6  GATGTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          201  GATC                 ^GATC
MspA1I         76  CAGCTG               C[AC]G^C[GT]G
NdeII         201  GATC                 ^GATC
PflFI         277  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         121  CACGTG               CAC^GTG
PmlI          121  CACGTG               CAC^GTG
PstI          177  CTGCAG               CTGCA^G
PvuII          76  CAGCTG               CAG^CTG
RsaI          108  GTAC                 GT^AC
SacI           64  GAGCTC               GAGCT^C
Sau3AI        201  GATC                 ^GATC
Tth111I       277  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AspHI          64  GAGCTC               G[AT]GC[AT]^C
              118  GTGCAC              
AvaII         126  GGTCC                G^G[AT]CC
              132  GGACC               
BcnI          129  CCCGG                CC^[GC]GG
              273  CCCGG               
BsiHKAI        64  GAGCTC               G[AT]GC[AT]^C
              118  GTGCAC              
BssHII        170  GCGCGC               G^CGCGC
              209  GCGCGC              
BssKI         129  CCCGG                ^CC[ACGT]GG
              273  CCCGG               
Cfr13I        126  GGTCC                G^G[ACGT]CC
              132  GGACC               
EaeI          251  TGGCCG               [CT]^GGCC[GA]
              311  CGGCCA              
MaeII          26  ACGT                 A^CGT
              122  ACGT                
MnlI           38  CCTC                 CCTC(7/6)
               57  CCTC                
MslI           21  CACCAACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              111  CATCGCGGTG          
NciI          129  CCCGG                CC^[GC]GG
              273  CCCGG               
NlaIII        141  CATG                 CATG^
              303  CATG                
PflMI          72  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              135  CCAGCTCATGG         
Sau96I        126  GGTCC                G^G[ACGT]CC
              132  GGACC               
ScrFI         129  CCCGG                CC^[ACGT]GG
              273  CCCGG               
SfaNI         191  GCATC                GCATC(5/9)
              266  GCATC               
SfcI          177  CTGCAG               C^T[GA][CT]AG
              225  CTACAG              
Van91I         72  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              135  CCAGCTCATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AccII       AciI        AluI        BbvI        BmyI        
BsiYI       BslI        Bsp1286I    BstUI       Cac8I       
CfoI        Fnu4HI      HaeIII      HapII       HhaI        
HinP1I      HpaII       ItaI        MspI        MvnI        
MwoI        NlaIV       TseI        
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