Restriction map for pF1KB8451
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

AccI        AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       ApoI        AseI        AsnI        Asp700I     
AspEI       AspI        AviII       AvrII       BbrPI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsiWI       BsmAI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
DraI        DraIII      Eam1105I    Eco47III    EcoNI       
EcoRI       FbaI        FseI        FspI        HincII      
HindII      HindIII     HinfI       HpaI        KspI        
MaeI        MamI        MfeI        MluI        MroI        
MseI        MunI        NcoI        NheI        NotI        
NruI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Tth111I     XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII         262  GACGTC               GACGT^C
Acc65I        560  GGTACC               G^GTACC
AocI          730  CCTGAGG              CC^T[ACGT]AGG
ApaI            6  GGGCCC               GGGCC^C
AscI          513  GGCGCGCC             GG^CGCGCC
Asp718I       560  GGTACC               G^GTACC
BamHI         494  GGATCC               G^GATCC
BbsI          265  GTCTTC               GAAGAC(2/6)
BpuAI         265  GTCTTC               GAAGAC(2/6)
BsgI          893  GTGCAG               GTGCAG(16/14)
BsiEI         428  CGGCCG               CG[GA][CT]^CG
BssSI         238  CACGAG               CACGAG(-5/-1)
BstEII        312  GGTGACC              G^GT[ACGT]ACC
BstPI         312  GGTGACC              G^GT[ACGT]ACC
BstYI         494  GGATCC               [GA]^GATC[CT]
Bsu36I        730  CCTGAGG              CC^T[ACGT]AGG
DrdI          880  GACCAGAGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          428  CGGCCG               C^GGCCG
EarI          664  CTCTTC               CTCTTC(1/4)
EclXI         428  CGGCCG               C^GGCCG
Eco52I        428  CGGCCG               C^GGCCG
Eco81I        730  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       312  GGTGACC              G^GT[ACGT]ACC
EcoRV         613  GATATC               GAT^ATC
EcoT22I       592  ATGCAT               ATGCA^T
FauI          407  GCGGG                CCCGC(4/6)
HgaI          127  GACGC                GACGC(5/10)
KpnI          560  GGTACC               GGTAC^C
Ksp632I       664  CTCTTC               CTCTTC(1/4)
MflI          494  GGATCC               [GA]^GATC[CT]
NdeI          965  CATATG               CA^TATG
NsiI          592  ATGCAT               ATGCA^T
PstI           70  CTGCAG               CTGCA^G
SfcI           70  CTGCAG               C^T[GA][CT]AG
SmaI          669  CCCGGG               CCC^GGG
SmlI          223  CTCAAG               C^T[CT][GA]AG
TaqI          287  TCGA                 T^CGA
Tsp509I       383  AATT                 ^AATT
TthHB8I       287  TCGA                 T^CGA
XhoII         494  GGATCC               [GA]^GATC[CT]
XmaCI         669  CCCGGG               C^CCGGG
XmaI          669  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AvaI          669  CCCGGG               C^[CT]CG[GA]G
              940  CCCGAG              
BalI          548  TGGCCA               TGG^CCA
              839  TGGCCA              
BbeI           97  GGCGCC               GGCGC^C
              328  GGCGCC              
BbvCI        1035  CCTCAGC              CCTCAGC(-5/-2)
              898  GCTGAGG             
BglI           60  GCCGCAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
               83  GCCCGGTGGGC         
BlpI          363  GCTGAGC              GC^T[ACGT]AGC
              640  GCTGAGC             
Bpu1102I      363  GCTGAGC              GC^T[ACGT]AGC
              640  GCTGAGC             
BsmFI         446  GGGAC                GGGAC(10/14)
              780  GTCCC               
BsoBI         669  CCCGGG               C^[CT]CG[GA]G
              940  CCCGAG              
BssHII        193  GCGCGC               G^CGCGC
              514  GCGCGC              
BstAPI         63  GCAGCGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
               72  GCAGGGGCTGC         
CelII         363  GCTGAGC              GC^T[ACGT]AGC
              640  GCTGAGC             
DsaI          623  CCGTGG               C^C[GA][CT]GG
              822  CCGTGG              
EcoT14I       852  CCTTGG               C^C[AT][AT]GG
              986  CCAAGG              
HphI          153  GGTGA                GGTGA(8/7)
              312  GGTGA               
KasI           97  GGCGCC               G^GCGCC
              328  GGCGCC              
MaeIII        313  GTGAC                ^GT[ACGT]AC
              878  GTGAC               
MluNI         548  TGGCCA               TGG^CCA
              839  TGGCCA              
MscI          548  TGGCCA               TGG^CCA
              839  TGGCCA              
MslI          318  CACCCTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              705  CATGGAAGTG          
NaeI           16  GCCGGC               GCC^GGC
              273  GCCGGC              
NarI           97  GGCGCC               GG^CGCC
              328  GGCGCC              
NgoMIV         16  GCCGGC               G^CCGGC
              273  GCCGGC              
PpuMI         446  GGGACCT              [GA]G^G[AT]CC[CT]
              803  AGGACCT             
SacI          487  GAGCTC               GAGCT^C
             1015  GAGCTC              
SfoI           97  GGCGCC               GGC^GCC
              328  GGCGCC              
StyI          852  CCTTGG               C^C[AT][AT]GG
              986  CCAAGG              
Tsp45I        313  GTGAC                ^GT[GC]AC
              878  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaHI       BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        Bsp1286I    BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       EaeI        EcoO109I    EcoRII      Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HpaII       ItaI        MaeII       
MboI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflMI       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       TseI        
TspRI       Van91I      
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