Restriction map for pF1KB8451
 (Linear) (Six-base) MAPSORT of: KIBB8451.seq  Check: 4523  from: 1  to: 1092



KIBB8451.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AatII G_ACGT'C

Cuts at:      0     266    1092 
   Size:        266     826
 
Acc65I G'GTAC_C

Cuts at:      0     560    1092 
   Size:        560     532
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     279     433     472     715     906    1092 
   Size:        253      26     154      39     243     191     186

  Fragments arranged by size: 

                 253     243     191     186     154      39      26
 
AlwNI CAG_nnn'CTG

Cuts at:      0      36      69      78     183     363     402     783    1092 
   Size:         36      33       9     105     180      39     381     309

  Fragments arranged by size: 

                 381     309     180     105      39      36      33       9
            
 
ApaI G_GGCC'C

Cuts at:      0      10    1092 
   Size:         10    1082
 
AscI GG'CGCG_CC

Cuts at:      0     514    1092 
   Size:        514     578
 
AvaI C'yCGr_G

Cuts at:      0     669     940    1092 
   Size:        669     271     152

  Fragments arranged by size: 

                 669     271     152
 
BamHI G'GATC_C

Cuts at:      0     494    1092 
   Size:        494     598
 
BanI G'GyrC_C

Cuts at:      0      57      97     328     560     567    1092 
   Size:         57      40     231     232       7     525

  Fragments arranged by size: 

                 525     232     231      57      40       7
 
BanII G_rGCy'C

Cuts at:      0      10      94     491    1019    1092 
   Size:         10      84     397     528      73

  Fragments arranged by size: 

                 528     397      84      73      10
 
BbeI G_GCGC'C

Cuts at:      0     101     332    1092 
   Size:        101     231     760

  Fragments arranged by size: 

                 760     231     101
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     258    1092 
   Size:        258     834
 
BbvCI CC'TCA_GC

Cuts at:      0     899    1036    1092 
   Size:        899     137      56

  Fragments arranged by size: 

                 899     137      56
 
BfrBI ATG'CAT

Cuts at:      0     594    1092 
   Size:        594     498
 
BglI GCCn_nnn'nGGC

Cuts at:      0      66      89    1092 
   Size:         66      23    1003

  Fragments arranged by size: 

                1003      66      23
 
BlpI GC'TnA_GC

Cuts at:      0     364     641    1092 
   Size:        364     277     451

  Fragments arranged by size: 

                 451     364     277
 
Bme1580I G_kGCm'C

Cuts at:      0      10     570     721    1092 
   Size:         10     560     151     371

  Fragments arranged by size: 

                 560     371     151      10
 
BmgBI CAC'GTC

Cuts at:      0     281    1092 
   Size:        281     811
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     634    1021    1092 
   Size:        634     387      71

  Fragments arranged by size: 

                 634     387      71
 
Bpu10I CC'TnA_GC

Cuts at:      0     490     808     899    1036    1092 
   Size:        490     318      91     137      56

  Fragments arranged by size: 

                 490     318     137      91      56
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208    1092 
   Size:        208     884
 
BsaHI Gr'CG_yC

Cuts at:      0      98     128     263     329    1092 
   Size:         98      30     135      66     763

  Fragments arranged by size: 

                 763     135      98      66      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     544     741    1018    1092 
   Size:        544     197     277      74

  Fragments arranged by size: 

                 544     277     197      74
 
BseYI C'CCAG_C

Cuts at:      0     457     724    1092 
   Size:        457     267     368

  Fragments arranged by size: 

                 457     368     267
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     914    1092 
   Size:        914     178
 
BsiEI CG_ry'CG

Cuts at:      0     431    1092 
   Size:        431     661
 
BsiHKAI G_wGCw'C

Cuts at:      0     377     491    1019    1092 
   Size:        377     114     528      73

  Fragments arranged by size: 

                 528     377     114      73
 
Bsp1286I G_dGCh'C

Cuts at:      0      10      94     377     491     570     721    1019    1092 
   Size:         10      84     283     114      79     151     298      73

  Fragments arranged by size: 

                 298     283     151     114      84      79      73      10
            
 
BsrFI r'CCGG_y

Cuts at:      0      16      26     273     538    1092 
   Size:         16      10     247     265     554

  Fragments arranged by size: 

                 554     265     247      16      10
 
BssHII G'CGCG_C

Cuts at:      0     193     514    1092 
   Size:        193     321     578

  Fragments arranged by size: 

                 578     321     193
 
BssSI C'ACGA_G

Cuts at:      0     238    1092 
   Size:        238     854
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      69      78    1092 
   Size:         69       9    1014

  Fragments arranged by size: 

                1014      69       9
 
BstEII G'GTnAC_C

Cuts at:      0     312    1092 
   Size:        312     780
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     324     465     837    1092 
   Size:        324     141     372     255

  Fragments arranged by size: 

                 372     324     255     141
 
BstYI r'GATC_y

Cuts at:      0     494    1092 
   Size:        494     598
 
Bsu36I CC'TnA_GG

Cuts at:      0     731    1092 
   Size:        731     361
 
BtgI C'CryG_G

Cuts at:      0     623     822    1092 
   Size:        623     199     270

  Fragments arranged by size: 

                 623     270     199
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     886    1092 
   Size:        886     206
 
EaeI y'GGCC_r

Cuts at:      0     275     428     548     839    1092 
   Size:        275     153     120     291     253

  Fragments arranged by size: 

                 291     275     253     153     120
 
EagI C'GGCC_G

Cuts at:      0     428    1092 
   Size:        428     664
 
EarI CTCTTCn'nnn_

Cuts at:      0     670    1092 
   Size:        670     422
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     538    1092 
   Size:        538     554
 
EcoICRI GAG'CTC

Cuts at:      0     489    1017    1092 
   Size:        489     528      75

  Fragments arranged by size: 

                 528     489      75
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     279     433     472     634     715     906    1021 
   Size:        253      26     154      39     162      81     191     115

Cuts at:   1021    1092 
   Size:         71

  Fragments arranged by size: 

                 253     191     162     154     115      81      71      39
                  26
 
EcoO109I rG'GnC_Cy

Cuts at:      0       7     447     660     804    1092 
   Size:          7     440     213     144     288

  Fragments arranged by size: 

                 440     288     213     144       7
 
EcoRV GAT'ATC

Cuts at:      0     615    1092 
   Size:        615     477
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     681    1092 
   Size:        681     411
 
HaeII r_GCGC'y

Cuts at:      0     101     332     364    1092 
   Size:        101     231      32     728

  Fragments arranged by size: 

                 728     231     101      32
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     604     636    1092 
   Size:        604      32     456

  Fragments arranged by size: 

                 604     456      32
 
KasI G'GCGC_C

Cuts at:      0      97     328    1092 
   Size:         97     231     764

  Fragments arranged by size: 

                 764     231      97
 
KpnI G_GTAC'C

Cuts at:      0     564    1092 
   Size:        564     528
 
MscI TGG'CCA

Cuts at:      0     550     841    1092 
   Size:        550     291     251

  Fragments arranged by size: 

                 550     291     251
 
MslI CAynn'nnrTG

Cuts at:      0     322     709    1092 
   Size:        322     387     383

  Fragments arranged by size: 

                 387     383     322
 
MspA1I CmG'CkG

Cuts at:      0      66     399     693     898    1092 
   Size:         66     333     294     205     194

  Fragments arranged by size: 

                 333     294     205     194      66
 
NaeI GCC'GGC

Cuts at:      0      18     275    1092 
   Size:         18     257     817

  Fragments arranged by size: 

                 817     257      18
 
NarI GG'CG_CC

Cuts at:      0      98     329    1092 
   Size:         98     231     763

  Fragments arranged by size: 

                 763     231      98
 
NdeI CA'TA_TG

Cuts at:      0     966    1092 
   Size:        966     126
 
NgoMIV G'CCGG_C

Cuts at:      0      16     273    1092 
   Size:         16     257     819

  Fragments arranged by size: 

                 819     257      16
 
NsiI A_TGCA'T

Cuts at:      0     596    1092 
   Size:        596     496
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     147     557     609    1092 
   Size:        147     410      52     483

  Fragments arranged by size: 

                 483     410     147      52
 
PfoI T'CCnGG_A

Cuts at:      0     497    1092 
   Size:        497     595
 
PpuMI rG'GwC_Cy

Cuts at:      0     447     804    1092 
   Size:        447     357     288

  Fragments arranged by size: 

                 447     357     288
 
PspOMI G'GGCC_C

Cuts at:      0       6    1092 
   Size:          6    1086
 
PstI C_TGCA'G

Cuts at:      0      74    1092 
   Size:         74    1018
 
PvuII CAG'CTG

Cuts at:      0     399     693     898    1092 
   Size:        399     294     205     194

  Fragments arranged by size: 

                 399     294     205     194
 
SacI G_AGCT'C

Cuts at:      0     491    1019    1092 
   Size:        491     528      73

  Fragments arranged by size: 

                 528     491      73
 
SfcI C'TryA_G

Cuts at:      0      70    1092 
   Size:         70    1022
 
SfoI GGC'GCC

Cuts at:      0      99     330    1092 
   Size:         99     231     762

  Fragments arranged by size: 

                 762     231      99
 
SmaI CCC'GGG

Cuts at:      0     671    1092 
   Size:        671     421
 
SmlI C'TyrA_G

Cuts at:      0     223    1092 
   Size:        223     869
 
StyI C'CwwG_G

Cuts at:      0     852     986    1092 
   Size:        852     134     106

  Fragments arranged by size: 

                 852     134     106
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     738    1092 
   Size:        738     354
 
TatI w'GTAC_w

Cuts at:      0     305     416    1092 
   Size:        305     111     676

  Fragments arranged by size: 

                 676     305     111
 
XmaI C'CCGG_G

Cuts at:      0     669    1092 
   Size:        669     423
 
ZraI GAC'GTC

Cuts at:      0     264    1092 
   Size:        264     828
 

 Enzymes that do cut:

    AatII   Acc65I     AcuI    AlwNI     ApaI     AscI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BpmI   Bpu10I    BpuEI    BsaHI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BsrFI   BssHII    BssSI   BstAPI
   BstEII    BstXI    BstYI   Bsu36I     BtgI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRV     FalI    HaeII
    Hin4I     KasI     KpnI     MscI     MslI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NsiI    PflMI     PfoI    PpuMI   PspOMI     PstI
    PvuII     SacI     SfcI     SfoI     SmaI     SmlI     StyI    TaqII
     TatI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     ApoI     AseI    AsiSI    AvrII
     BaeI     BcgI    BciVI     BclI    BglII     BmrI     BmtI     BplI
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI    BstBI  BstZ17I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII    EcoNI    EcoRI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     MfeI     MluI
     MmeI     NcoI     NheI     NotI     NruI     NspI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmnI
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