Restriction map for pF1KB8448
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvrII       BamHI       BbeI        BbvCI       
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsaI        BsgI        BsiHKAI     BsiWI       BsmI        
Bsp1407I    BspDI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssHII      Bst1107I    BstBI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DrdI        Eam1105I    Eco81I      EcoRI       EcoRV       
EcoT22I     FbaI        FseI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
MaeI        MamI        MfeI        MluI        MseI        
MunI        NarI        NcoI        NdeI        NheI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SalI        
SbfI        ScaI        SexAI       SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        TfiI        Tru9I       
Tsp509I     Tth111I     XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccIII       1241  TCCGGA               T^CCGGA
AflIII         86  ACGTGT               A^C[GA][CT]GT
AlwI          933  GATCC                GGATC(4/5)
Aor51HI       119  AGCGCT               AGC^GCT
Asp700I       354  GAACGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         1049  CCCGGG               C^[CT]CG[GA]G
AviII         626  TGCGCA               TGC^GCA
BbsI          964  GTCTTC               GAAGAC(2/6)
BcgI           53  GCAACCGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI         964  GTCTTC               GAAGAC(2/6)
BseAI        1241  TCCGGA               T^CCGGA
BsiEI        1181  CGGCCG               CG[GA][CT]^CG
BsmBI         388  GAGACG               CGTCTC(1/5)
BsmFI         719  GGGAC                GGGAC(10/14)
BsoBI        1049  CCCGGG               C^[CT]CG[GA]G
BspEI        1241  TCCGGA               T^CCGGA
BsrFI        1027  GCCGGC               [GA]^CCGG[CT]
BssSI         787  CACGAG               CACGAG(-5/-1)
BstEII        253  GGTGACC              G^GT[ACGT]ACC
BstPI         253  GGTGACC              G^GT[ACGT]ACC
BstXI         143  CCACCTGCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         932  AGATCC               [GA]^GATC[CT]
Cfr10I       1027  GCCGGC               [GA]^CCGG[CT]
DdeI          103  CTGAG                C^T[ACGT]AG
DraIII        637  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         1181  CGGCCG               C^GGCCG
EclXI        1181  CGGCCG               C^GGCCG
Eco47III      119  AGCGCT               AGC^GCT
Eco52I       1181  CGGCCG               C^GGCCG
EcoNI        1313  CCTACCGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       253  GGTGACC              G^GT[ACGT]ACC
EcoT14I      1220  CCAAGG               C^C[AT][AT]GG
FspI          626  TGCGCA               TGC^GCA
MflI          932  AGATCC               [GA]^GATC[CT]
MroI         1241  TCCGGA               T^CCGGA
NaeI         1027  GCCGGC               GCC^GGC
NgoMIV       1027  GCCGGC               G^CCGGC
NotI         1180  GCGGCCGC             GC^GGCCGC
PflMI         636  CCACATTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         719  GGGACCT              [GA]G^G[AT]CC[CT]
PstI         1057  CTGCAG               CTGCA^G
PvuII        1172  CAGCTG               CAG^CTG
SapI          975  GAAGAGC              GCTCTTC(1/4)
SfcI         1057  CTGCAG               C^T[GA][CT]AG
SfiI         1182  GGCCGCGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         1049  CCCGGG               CCC^GGG
SphI         1280  GCATGC               GCATG^C
StuI         1197  AGGCCT               AGG^CCT
StyI         1220  CCAAGG               C^C[AT][AT]GG
TspRI        1104  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I        636  CCACATTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         932  AGATCC               [GA]^GATC[CT]
XmaCI        1049  CCCGGG               C^CCGGG
XmaI         1049  CCCGGG               C^CCGGG
XmnI          354  GAACGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AfaI           90  GTAC                 GT^AC
              699  GTAC                
ApaI          291  GGGCCC               GGGCC^C
              659  GGGCCC              
BalI          515  TGGCCA               TGG^CCA
              923  TGGCCA              
BanII         291  GGGCCC               G[GA]GC[CT]^C
              659  GGGCCC              
BsaWI         416  TCCGGT               [AT]^CCGG[AT]
             1241  TCCGGA              
BsmAI         388  GAGAC                GTCTC(1/5)
              202  GTCTC               
BspHI         176  TCATGA               T^CATGA
              497  TCATGA              
BsrI          465  CCAGT                ACTGG(1/-1)
             1103  CCAGT               
DraII         290  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              719  GGGACCT             
EcoO109I      290  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              719  GGGACCT             
HgaI          390  GACGC                GACGC(5/10)
              482  GCGTC               
KspI          188  CCGCGG               CCGC^GG
             1184  CCGCGG              
MaeIII        254  GTGAC                ^GT[ACGT]AC
              424  GTGAC               
MluNI         515  TGGCCA               TGG^CCA
              923  TGGCCA              
MscI          515  TGGCCA               TGG^CCA
              923  TGGCCA              
NspI          170  ACATGC               [GA]CATG^[CT]
             1280  GCATGC              
RcaI          176  TCATGA               T^CATGA
              497  TCATGA              
RsaI           90  GTAC                 GT^AC
              699  GTAC                
SacII         188  CCGCGG               CCGC^GG
             1184  CCGCGG              
Tsp45I        254  GTGAC                ^GT[GC]AC
              424  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AccII       AciI        AluI        AvaII       BanI        
BbrPI       BbvI        BcnI        BglI        BmyI        
BpmI        BsaAI       BsaJI       BseRI       BsiYI       
BslI        Bsp1286I    BspMI       BssKI       BstAPI      
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DpnII       DsaI        EaeI        EarI        
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MboI        MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PmaCI       
PmlI        Sau3AI      Sau96I      ScrFI       SfaNI       
TaqI        TseI        TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com