Restriction map for pF1KB8448
 (Linear) (Six-base) MAPSORT of: KIBB8448.seq  Check: 9739  from: 1  to: 1335



KIBB8448.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     154    1335 
   Size:        154    1181
 
AfeI AGC'GCT

Cuts at:      0     121    1335 
   Size:        121    1214
 
AflIII A'CryG_T

Cuts at:      0      86    1335 
   Size:         86    1249
 
AleI CACnn'nnGTG

Cuts at:      0     148    1335 
   Size:        148    1187
 
ApaI G_GGCC'C

Cuts at:      0     295     663    1335 
   Size:        295     368     672

  Fragments arranged by size: 

                 672     368     295
 
AvaI C'yCGr_G

Cuts at:      0    1049    1335 
   Size:       1049     286
 
BanI G'GyrC_C

Cuts at:      0     276     367     505    1335 
   Size:        276      91     138     830

  Fragments arranged by size: 

                 830     276     138      91
 
BanII G_rGCy'C

Cuts at:      0     295     663    1335 
   Size:        295     368     672

  Fragments arranged by size: 

                 672     368     295
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     957    1335 
   Size:        957     378
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      42      76    1335 
   Size:         42      34    1259

  Fragments arranged by size: 

                1259      42      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      20     325     523    1189    1335 
   Size:         20     305     198     666     146

  Fragments arranged by size: 

                 666     305     198     146      20
 
Bme1580I G_kGCm'C

Cuts at:      0      38     295     439     663    1335 
   Size:         38     257     144     224     672

  Fragments arranged by size: 

                 672     257     224     144      38
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1097    1335 
   Size:       1097     238
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     856     889    1228    1270    1294    1335 
   Size:        280     576      33     339      42      24      41

  Fragments arranged by size: 

                 576     339     280      42      41      33      24
 
Bpu10I CC'TnA_GC

Cuts at:      0     103    1335 
   Size:        103    1232
 
BsaAI yAC'GTr

Cuts at:      0      33     351     654    1335 
   Size:         33     318     303     681

  Fragments arranged by size: 

                 681     318     303      33
 
BsaWI w'CCGG_w

Cuts at:      0     416    1241    1335 
   Size:        416     825      94

  Fragments arranged by size: 

                 825     416      94
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     682     712     823     853    1294    1324    1335 
   Size:        682      30     111      30     441      30      11

  Fragments arranged by size: 

                 682     441     111      30      30      30      11
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     214     445     844     985    1168    1177    1180    1240 
   Size:        214     231     399     141     183       9       3      60

Cuts at:   1240    1335 
   Size:         95

  Fragments arranged by size: 

                 399     231     214     183     141      95      60       9
                   3
 
BseYI C'CCAG_C

Cuts at:      0     662    1335 
   Size:        662     673
 
BsiEI CG_ry'CG

Cuts at:      0    1184    1335 
   Size:       1184     151
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     382    1335 
   Size:        382     953
 
Bsp1286I G_dGCh'C

Cuts at:      0      38     295     439     663    1335 
   Size:         38     257     144     224     672

  Fragments arranged by size: 

                 672     257     224     144      38
 
BspEI T'CCGG_A

Cuts at:      0    1241    1335 
   Size:       1241      94
 
BspHI T'CATG_A

Cuts at:      0     176     497    1335 
   Size:        176     321     838

  Fragments arranged by size: 

                 838     321     176
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     154     632     710    1335 
   Size:        154     478      78     625

  Fragments arranged by size: 

                 625     478     154      78
 
BsrFI r'CCGG_y

Cuts at:      0    1027    1335 
   Size:       1027     308
 
BssSI C'ACGA_G

Cuts at:      0     787    1335 
   Size:        787     548
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     552     599    1037    1335 
   Size:        552      47     438     298

  Fragments arranged by size: 

                 552     438     298      47
 
BstEII G'GTnAC_C

Cuts at:      0     253    1335 
   Size:        253    1082
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     150    1335 
   Size:        150    1185
 
BstYI r'GATC_y

Cuts at:      0     932    1335 
   Size:        932     403
 
BtgI C'CryG_G

Cuts at:      0     188     249     887    1184    1335 
   Size:        188      61     638     297     151

  Fragments arranged by size: 

                 638     297     188     151      61
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     435    1335 
   Size:        435     900
 
DraIII CAC_nnn'GTG

Cuts at:      0     642    1335 
   Size:        642     693
 
EaeI y'GGCC_r

Cuts at:      0      21      75     515     890     923    1025    1181    1190 
   Size:         21      54     440     375      33     102     156       9

Cuts at:   1190    1335 
   Size:        145

  Fragments arranged by size: 

                 440     375     156     145     102      54      33      21
                   9
 
EagI C'GGCC_G

Cuts at:      0    1181    1335 
   Size:       1181     154
 
EarI CTCTTCn'nnn_

Cuts at:      0     232     970    1145    1335 
   Size:        232     738     175     190

  Fragments arranged by size: 

                 738     232     190     175
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      57     145     326    1335 
   Size:         57      88     181    1009

  Fragments arranged by size: 

                1009     181      88      57
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     856     889    1228    1270    1294    1335 
   Size:        280     576      33     339      42      24      41

  Fragments arranged by size: 

                 576     339     280      42      41      33      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1317    1335 
   Size:       1317      18
 
EcoO109I rG'GnC_Cy

Cuts at:      0     291     720    1335 
   Size:        291     429     615

  Fragments arranged by size: 

                 615     429     291
 
FspI TGC'GCA

Cuts at:      0     628    1335 
   Size:        628     707
 
HaeII r_GCGC'y

Cuts at:      0      51     123     168    1335 
   Size:         51      72      45    1167

  Fragments arranged by size: 

                1167      72      51      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     823     855    1294    1326    1335 
   Size:        823      32     439      32       9

  Fragments arranged by size: 

                 823     439      32      32       9
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     821    1335 
   Size:        821     514
 
MscI TGG'CCA

Cuts at:      0     517     925    1335 
   Size:        517     408     410

  Fragments arranged by size: 

                 517     410     408
 
MslI CAynn'nnrTG

Cuts at:      0     148     175     349     454     511     580     892    1243 
   Size:        148      27     174     105      57      69     312     351

Cuts at:   1243    1335 
   Size:         92

  Fragments arranged by size: 

                 351     312     174     148     105      92      69      57
                  27
 
MspA1I CmG'CkG

Cuts at:      0     190     441     717    1174    1186    1299    1335 
   Size:        190     251     276     457      12     113      36

  Fragments arranged by size: 

                 457     276     251     190     113      36      12
 
NaeI GCC'GGC

Cuts at:      0    1029    1335 
   Size:       1029     306
 
NgoMIV G'CCGG_C

Cuts at:      0    1027    1335 
   Size:       1027     308
 
NotI GC'GGCC_GC

Cuts at:      0    1181    1335 
   Size:       1181     154
 
NspI r_CATG'y

Cuts at:      0     174    1284    1335 
   Size:        174    1110      51

  Fragments arranged by size: 

                1110     174      51
 
PasI CC'CwG_GG

Cuts at:      0     271    1335 
   Size:        271    1064
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     642    1335 
   Size:        642     693
 
PfoI T'CCnGG_A

Cuts at:      0     866    1335 
   Size:        866     469
 
PmlI CAC'GTG

Cuts at:      0      33     351     654    1335 
   Size:         33     318     303     681

  Fragments arranged by size: 

                 681     318     303      33
 
PpuMI rG'GwC_Cy

Cuts at:      0     720    1335 
   Size:        720     615
 
PspOMI G'GGCC_C

Cuts at:      0     291     659    1335 
   Size:        291     368     676

  Fragments arranged by size: 

                 676     368     291
 
PstI C_TGCA'G

Cuts at:      0    1061    1335 
   Size:       1061     274
 
PvuII CAG'CTG

Cuts at:      0    1174    1335 
   Size:       1174     161
 
SacII CC_GC'GG

Cuts at:      0     191    1187    1335 
   Size:        191     996     148

  Fragments arranged by size: 

                 996     191     148
 
SapI GCTCTTCn'nnn_

Cuts at:      0     970    1335 
   Size:        970     365
 
SfcI C'TryA_G

Cuts at:      0    1057    1335 
   Size:       1057     278
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1189    1335 
   Size:       1189     146
 
SmaI CCC'GGG

Cuts at:      0    1051    1335 
   Size:       1051     284
 
SphI G_CATG'C

Cuts at:      0    1284    1335 
   Size:       1284      51
 
StuI AGG'CCT

Cuts at:      0    1199    1335 
   Size:       1199     136
 
StyI C'CwwG_G

Cuts at:      0    1220    1335 
   Size:       1220     115
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     355     916    1335 
   Size:        355     561     419

  Fragments arranged by size: 

                 561     419     355
 
TatI w'GTAC_w

Cuts at:      0      89    1335 
   Size:         89    1246
 
XmaI C'CCGG_G

Cuts at:      0    1049    1335 
   Size:       1049     286
 
XmnI GAAnn'nnTTC

Cuts at:      0     358    1335 
   Size:        358     977
 

 Enzymes that do cut:

     AarI     AfeI   AflIII     AleI     ApaI     AvaI     BanI    BanII
     BbsI     BcgI     BglI Bme1580I     BmrI     BpmI   Bpu10I    BsaAI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrFI    BssSI   BstAPI   BstEII    BstXI    BstYI
     BtgI    BtgZI   DraIII     EaeI     EagI     EarI     EciI  Eco57MI
    EcoNI EcoO109I     FspI    HaeII    Hin4I     MmeI     MscI     MslI
   MspA1I     NaeI   NgoMIV     NotI     NspI     PasI    PflMI     PfoI
     PmlI    PpuMI   PspOMI     PstI    PvuII    SacII     SapI     SfcI
     SfiI     SmaI     SphI     StuI     StyI    TaqII     TatI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI     AhdI
     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI    BciVI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaBI
    BsaHI     BsgI  BsiHKAI    BsiWI     BsmI    BsrBI    BsrDI    BsrGI
   BssHII    BstBI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI
     DrdI  EcoICRI    EcoRI    EcoRV     FalI     FseI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NarI     NcoI
     NdeI     NheI     NruI     NsiI     PacI     PciI     PmeI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
    SanDI     SbfI     ScaI    SexAI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SrfI     SspI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     ZraI
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