Restriction map for pF1KB8444
 (Linear) (Six-base) MAPSORT of: KIBB8444.seq  Check: 8765  from: 1  to: 909



KIBB8444.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     136     909 
   Size:        136     773
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     488     909 
   Size:        488     421
 
AlwNI CAG_nnn'CTG

Cuts at:      0     607     909 
   Size:        607     302
 
ApoI r'AATT_y

Cuts at:      0     229     579     909 
   Size:        229     350     330

  Fragments arranged by size: 

                 350     330     229
 
BamHI G'GATC_C

Cuts at:      0     596     909 
   Size:        596     313
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     425     909 
   Size:        425     484
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     323     909 
   Size:        323     586
 
BfrBI ATG'CAT

Cuts at:      0     616     909 
   Size:        616     293
 
Bme1580I G_kGCm'C

Cuts at:      0     489     909 
   Size:        489     420
 
BmgBI CAC'GTC

Cuts at:      0     870     909 
   Size:        870      39
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     274     909 
   Size:         67     207     635

  Fragments arranged by size: 

                 635     207      67
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     177     909 
   Size:        177     732
 
BsaWI w'CCGG_w

Cuts at:      0     658     909 
   Size:        658     251
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     624     654     909 
   Size:        624      30     255

  Fragments arranged by size: 

                 624     255      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      12     632     909 
   Size:         12     620     277

  Fragments arranged by size: 

                 620     277      12
 
Bsp1286I G_dGCh'C

Cuts at:      0     489     909 
   Size:        489     420
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     136     359     431     615     657     909 
   Size:        136     223      72     184      42     252

  Fragments arranged by size: 

                 252     223     184     136      72      42
 
BsrBI CCG'CTC

Cuts at:      0      32     909 
   Size:         32     877
 
BsrFI r'CCGG_y

Cuts at:      0      51     243     909 
   Size:         51     192     666

  Fragments arranged by size: 

                 666     192      51
 
BstYI r'GATC_y

Cuts at:      0     266     331     443     547     596     909 
   Size:        266      65     112     104      49     313

  Fragments arranged by size: 

                 313     266     112     104      65      49
 
BtgI C'CryG_G

Cuts at:      0      96     909 
   Size:         96     813
 
DraI TTT'AAA

Cuts at:      0     432     909 
   Size:        432     477
 
EaeI y'GGCC_r

Cuts at:      0     501     816     909 
   Size:        501     315      93

  Fragments arranged by size: 

                 501     315      93
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      24     909 
   Size:         24     885
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     274     488     909 
   Size:         67     207     214     421

  Fragments arranged by size: 

                 421     214     207      67
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0       9     909 
   Size:          9     900
 
EcoRI G'AATT_C

Cuts at:      0     579     909 
   Size:        579     330
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     780     909 
   Size:        780     129
 
HaeII r_GCGC'y

Cuts at:      0      96     909 
   Size:         96     813
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     624     656     909 
   Size:        624      32     253

  Fragments arranged by size: 

                 624     253      32
 
HindIII A'AGCT_T

Cuts at:      0     538     909 
   Size:        538     371
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     608     909 
   Size:        608     301
 
MscI TGG'CCA

Cuts at:      0     818     909 
   Size:        818      91
 
MslI CAynn'nnrTG

Cuts at:      0     802     909 
   Size:        802     107
 
NaeI GCC'GGC

Cuts at:      0     245     909 
   Size:        245     664
 
NgoMIV G'CCGG_C

Cuts at:      0     243     909 
   Size:        243     666
 
NsiI A_TGCA'T

Cuts at:      0     618     909 
   Size:        618     291
 
PasI CC'CwG_GG

Cuts at:      0     133     909 
   Size:        133     776
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     276     909 
   Size:        276     633
 
PstI C_TGCA'G

Cuts at:      0     654     909 
   Size:        654     255
 
RsrII CG'GwC_CG

Cuts at:      0      27     909 
   Size:         27     882
 
SfcI C'TryA_G

Cuts at:      0     356     650     909 
   Size:        356     294     259

  Fragments arranged by size: 

                 356     294     259
 
StyI C'CwwG_G

Cuts at:      0     764     909 
   Size:        764     145
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     360     909 
   Size:        360     549
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     ApoI    BamHI     BbsI    BciVI    BfrBI
 Bme1580I    BmgBI     BpmI     BsaI    BsaWI    BsaXI    BseRI Bsp1286I
    BspMI    BsrBI    BsrFI    BstYI     BtgI     DraI     EaeI     EciI
  Eco57MI    EcoNI    EcoRI     FalI    HaeII    Hin4I  HindIII     MmeI
     MscI     MslI     NaeI   NgoMIV     NsiI     PasI    PflMI     PstI
    RsrII     SfcI     StyI    TaqII

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI
     BcgI     BclI     BglI    BglII     BlpI     BmrI     BmtI     BplI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EarI
  EcoICRI EcoO109I    EcoRV     FseI     FspI    FspAI   HincII     HpaI
     KasI     KpnI     MfeI     MluI   MspA1I     NarI     NcoI     NdeI
     NheI     NotI     NruI     NspI     PacI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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