Restriction map for
pF1KB8441
(Circular) (Six-base) MAPSORT of: pF1KB8441.seq Check: 8565 from: 1 to: 3593
pF1KB8441 3593 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 584 584
Size: 3593
AccI GT'mk_AC
Cuts at: 631 631
Size: 3593
Acc65I G'GTAC_C
Cuts at: 609 609
Size: 3593
AclI AA'CG_TT
Cuts at: 3433 3433
Size: 3593
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 233 1418 1850 2203 2865 134
Size: 99 1185 432 353 662 862
Fragments arranged by size:
1185 862 662 432 353 99
AflII C'TTAA_G
Cuts at: 448 448
Size: 3593
AflIII A'CryG_T
Cuts at: 945 2745 3026 945
Size: 1800 281 1512
Fragments arranged by size:
1800 1512 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 475 507 475
Size: 32 3561
AlwNI CAG_nnn'CTG
Cuts at: 171 516 898 2336 171
Size: 345 382 1438 1428
Fragments arranged by size:
1438 1428 382 345
ApaI G_GGCC'C
Cuts at: 330 330
Size: 3593
ApaLI G'TGCA_C
Cuts at: 2431 2431
Size: 3593
ApoI r'AATT_y
Cuts at: 597 2946 3202 597
Size: 2349 256 988
Fragments arranged by size:
2349 988 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3593
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3593
AvaI C'yCGr_G
Cuts at: 487 613 487
Size: 126 3467
BamHI G'GATC_C
Cuts at: 618 618
Size: 3593
BanI G'GyrC_C
Cuts at: 609 1023 1271 1306 609
Size: 414 248 35 2896
Fragments arranged by size:
2896 414 248 35
BanII G_rGCy'C
Cuts at: 330 607 1637 330
Size: 277 1030 2286
Fragments arranged by size:
2286 1030 277
BbeI G_GCGC'C
Cuts at: 1027 1275 1027
Size: 248 3345
BciVI GTATCCnnnnn_n'
Cuts at: 1491 2547 3593 1491
Size: 1056 1046 1491
Fragments arranged by size:
1491 1056 1046
BclI T'GATC_A
Cuts at: 1113 1113
Size: 3593
BfrBI ATG'CAT
Cuts at: 257 257
Size: 3593
BglII A'GATC_T
Cuts at: 1108 3145 1108
Size: 2037 1556
BlpI GC'TnA_GC
Cuts at: 559 705 559
Size: 146 3447
Bme1580I G_kGCm'C
Cuts at: 330 1218 1311 2435 330
Size: 888 93 1124 1488
Fragments arranged by size:
1488 1124 888 93
BmgBI CAC'GTC
Cuts at: 315 315
Size: 3593
BmrI ACTGGGnnnn_n'
Cuts at: 805 886 1221 3365 805
Size: 81 335 2144 1033
Fragments arranged by size:
2144 1033 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1960 1960
Size: 3593
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 114 492 114
Size: 378 3215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 769 1648 2362 2660 568
Size: 201 879 714 298 1501
Fragments arranged by size:
1501 879 714 298 201
BsaI GGTCTCn'nnnn_
Cuts at: 142 3297 142
Size: 3155 438
BsaAI yAC'GTr
Cuts at: 946 946
Size: 3593
BsaBI GATnn'nnATC
Cuts at: 6 1107 3144 6
Size: 1101 2037 455
Fragments arranged by size:
2037 1101 455
BsaHI Gr'CG_yC
Cuts at: 1024 1272 1974 3470 1024
Size: 248 702 1496 1147
Fragments arranged by size:
1496 1147 702 248
BsaWI w'CCGG_w
Cuts at: 782 1303 1981 2392 2539 2813 782
Size: 521 678 411 147 274 1562
Fragments arranged by size:
1562 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 341 1018 1776 2441 2849 341
Size: 677 758 665 408 1085
Fragments arranged by size:
1085 758 677 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 556
Size: 3593
BsiEI CG_ry'CG
Cuts at: 104 1135 1181 2411 104
Size: 1031 46 1230 1286
Fragments arranged by size:
1286 1230 1031 46
BsiHKAI G_wGCw'C
Cuts at: 607 1385 1575 2435 607
Size: 778 190 860 1765
Fragments arranged by size:
1765 860 778 190
BsmI GAATG_Cn'
Cuts at: 259 259
Size: 3593
Bsp1286I G_dGCh'C
Cuts at: 330 607 1218 1311 1385 1575 1637 2435 330
Size: 277 611 93 74 190 62 798 1488
Fragments arranged by size:
1488 798 611 277 190 93 74 62
BspHI T'CATG_A
Cuts at: 2025 2025
Size: 3593
BspMI ACCTGCnnnn'nnnn_
Cuts at: 584 643 857 1159 1540 584
Size: 59 214 302 381 2637
Fragments arranged by size:
2637 381 302 214 59
BsrBI CCG'CTC
Cuts at: 50 1885 1939 3423 3588 50
Size: 1835 54 1484 165 55
Fragments arranged by size:
1835 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1505 1505
Size: 3593
BsrFI r'CCGG_y
Cuts at: 1591 1772 1591
Size: 181 3412
BssSI C'ACGA_G
Cuts at: 1864 2572 1864
Size: 708 2885
BstAPI GCAn_nnn'nTGC
Cuts at: 579 970 579
Size: 391 3202
BstBI TT'CG_AA
Cuts at: 1954 1954
Size: 3593
BstYI r'GATC_y
Cuts at: 1 475 618 1085 1108 1443 1689 1995 2007
Size: 474 143 467 23 335 246 306 12
Cuts at: 2007 2093 3145 1
Size: 86 1052 449
Fragments arranged by size:
1052 474 467 449 335 306 246 143
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 322 322
Size: 3593
BtgI C'CryG_G
Cuts at: 185 185
Size: 3593
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1543 1724 1887 1543
Size: 181 163 3249
Fragments arranged by size:
3249 181 163
BtsI GCAGTG_nn'
Cuts at: 273 836 273
Size: 563 3030
DraI TTT'AAA
Cuts at: 592 592
Size: 3593
DrdI GACnn_nn'nnGTC
Cuts at: 1299 2643 1299
Size: 1344 2249
EaeI y'GGCC_r
Cuts at: 356 1178 1352 1743 1770 3115 356
Size: 822 174 391 27 1345 834
Fragments arranged by size:
1345 834 822 391 174 27
EagI C'GGCC_G
Cuts at: 1178 1178
Size: 3593
EarI CTCTTCn'nnn_
Cuts at: 209 1616 1826 209
Size: 1407 210 1976
Fragments arranged by size:
1976 1407 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1801 2543 2689 3403 1801
Size: 742 146 714 1991
Fragments arranged by size:
1991 742 714 146
EcoICRI GAG'CTC
Cuts at: 605 605
Size: 3593
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 114 134 233 492 1418 1850 2203 2865 114
Size: 20 99 259 926 432 353 662 842
Fragments arranged by size:
926 842 662 432 353 259 99 20
EcoO109I rG'GnC_Cy
Cuts at: 219 326 327 491 567 732 219
Size: 107 1 164 76 165 3080
Fragments arranged by size:
3080 165 164 107 76 1
EcoRI G'AATT_C
Cuts at: 597 597
Size: 3593
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1612 1612
Size: 3593
FspI TGC'GCA
Cuts at: 1374 1374
Size: 3593
HaeII r_GCGC'y
Cuts at: 526 544 1027 1275 2505 3276 526
Size: 18 483 248 1230 771 843
Fragments arranged by size:
1230 843 771 483 248 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1435 1465 1467 1497 1435
Size: 30 2 30 3531
Fragments arranged by size:
3531 30 30 2
HincII GTy'rAC
Cuts at: 632 632
Size: 3593
KasI G'GCGC_C
Cuts at: 1023 1271 1023
Size: 248 3345
KpnI G_GTAC'C
Cuts at: 613 613
Size: 3593
MluI A'CGCG_T
Cuts at: 3026 3026
Size: 3593
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2352 2536 2352
Size: 184 3409
MscI TGG'CCA
Cuts at: 358 1354 358
Size: 996 2597
MslI CAynn'nnrTG
Cuts at: 142 1582 1709 142
Size: 1440 127 2026
Fragments arranged by size:
2026 1440 127
MspA1I CmG'CkG
Cuts at: 555 563 704 1018 1378 2162 2407 555
Size: 8 141 314 360 784 245 1741
Fragments arranged by size:
1741 784 360 314 245 141 8
NaeI GCC'GGC
Cuts at: 1774 1774
Size: 3593
NarI GG'CG_CC
Cuts at: 1024 1272 1024
Size: 248 3345
NcoI C'CATG_G
Cuts at: 185 185
Size: 3593
NgoMIV G'CCGG_C
Cuts at: 1772 1772
Size: 3593
NsiI A_TGCA'T
Cuts at: 259 259
Size: 3593
NspI r_CATG'y
Cuts at: 646 1146 2749 646
Size: 500 1603 1490
Fragments arranged by size:
1603 1490 500
PciI A'CATG_T
Cuts at: 2745 2745
Size: 3593
PfoI T'CCnGG_A
Cuts at: 170 170
Size: 3593
PmeI GTTT'AAAC
Cuts at: 592 592
Size: 3593
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2039 2071 2039
Size: 32 3561
PpuMI rG'GwC_Cy
Cuts at: 219 491 219
Size: 272 3321
PspOMI G'GGCC_C
Cuts at: 326 326
Size: 3593
PstI C_TGCA'G
Cuts at: 640 1325 640
Size: 685 2908
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3593
PvuII CAG'CTG
Cuts at: 1018 1378 1018
Size: 360 3233
RsrII CG'GwC_CG
Cuts at: 1788 1788
Size: 3593
SacI G_AGCT'C
Cuts at: 607 607
Size: 3593
SalI G'TCGA_C
Cuts at: 630 630
Size: 3593
SapI GCTCTTCn'nnn_
Cuts at: 1616 1826 1616
Size: 210 3383
SbfI CC_TGCA'GG
Cuts at: 640 640
Size: 3593
ScaI AGT'ACT
Cuts at: 3110 3110
Size: 3593
SfcI C'TryA_G
Cuts at: 33 636 1321 2289 2480 33
Size: 603 685 968 191 1146
Fragments arranged by size:
1146 968 685 603 191
SfoI GGC'GCC
Cuts at: 1025 1273 1025
Size: 248 3345
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3593
SmaI CCC'GGG
Cuts at: 489 615 489
Size: 126 3467
SmlI C'TyrA_G
Cuts at: 448 583 748 1663 2377 2639 448
Size: 135 165 915 714 262 1402
Fragments arranged by size:
1402 915 714 262 165 135
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3593
SphI G_CATG'C
Cuts at: 646 1146 646
Size: 500 3093
StyI C'CwwG_G
Cuts at: 185 727 1081 185
Size: 542 354 2697
Fragments arranged by size:
2697 542 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 486 1176 1309 1768 1977 486
Size: 690 133 459 209 2102
Fragments arranged by size:
2102 690 459 209 133
TatI w'GTAC_w
Cuts at: 3108 3108
Size: 3593
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3110 3142 3110
Size: 32 3561
Tth111I GACn'n_nGTC
Cuts at: 1390 1390
Size: 3593
XbaI T'CTAG_A
Cuts at: 624 624
Size: 3593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 366 1088 366
Size: 722 2871
XmaI C'CCGG_G
Cuts at: 487 613 487
Size: 126 3467
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AleI AlfI AscI AvrII
BaeI BbsI BbvCI BcgI BglI BmtI Bpu10I BsaXI
BseRI BsiWI BsmBI BspEI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PasI PflMI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI