Restriction map for pF1KB8432
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AcyI        
AflII       AgeI        ApoI        AscI        AseI        
AsnI        Asp718I     AviII       AvrII       BbeI        
BbrPI       BbsI        BbvCI       BciVI       BclI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaHI       BsiWI       BsrBI       BsrDI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstXI       BstZ17I     CelII       CpoI        EagI        
EclXI       Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MfeI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccIII        626  TCCGGA               T^CCGGA
Asp700I      1920  GAATCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          522  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         232  GGATCC               G^GATCC
BcgI          750  CGAGACAGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        2576  AGATCT               A^GATCT
BsaBI        1074  GATTTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         626  TCCGGA               T^CCGGA
BsiEI         111  CGACCG               CG[GA][CT]^CG
BsmI         1357  GCATTC               GAATGC(1/-1)
Bsp1407I      737  TGTACA               T^GTACA
BspDI         844  ATCGAT               AT^CGAT
BspEI         626  TCCGGA               T^CCGGA
BspLU11I      740  ACATGT               A^CATGT
BsrGI         737  TGTACA               T^GTACA
BstAPI        831  GCACGGCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1576  TTCGAA               TT^CGAA
ClaI          844  ATCGAT               AT^CGAT
DraI         1204  TTTAAA               TTT^AAA
DraIII        832  CACGGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2290  GACCGGACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI           22  CTCTTC               CTCTTC(1/4)
Ksp632I        22  CTCTTC               CTCTTC(1/4)
MamI         1074  GATTTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         2605  ACGCGT               A^CGCGT
MroI          626  TCCGGA               T^CCGGA
NspV         1576  TTCGAA               TT^CGAA
PaeR7I        748  CTCGAG               C^TCGAG
PflFI         522  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SexAI        2705  ACCTGGT              A^CC[AT]GGT
SfuI         1576  TTCGAA               TT^CGAA
SspBI         737  TGTACA               T^GTACA
Tth111I       522  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          748  CTCGAG               C^TCGAG
XmnI         1920  GAATCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI         1752  GTCTAC               GT^[AC][GT]AC
             2029  GTCTAC              
AccII        1466  CGCG                 CG^CG
             2606  CGCG                
AflIII        740  ACATGT               A^C[GA][CT]GT
             2605  ACGCGT              
Alw44I        160  GTGCAC               G^TGCAC
             1800  GTGCAC              
AocI          454  CCTGAGG              CC^T[ACGT]AGG
             1102  CCTGAGG             
Aor51HI       632  AGCGCT               AGC^GCT
             1682  AGCGCT              
ApaLI         160  GTGCAC               G^TGCAC
             1800  GTGCAC              
BfaI         2731  CTAG                 C^TAG
             2785  CTAG                
BsaAI        2032  TACGTG               [CT]AC^GT[GA]
             2632  TACGTG              
BsaI          677  GAGACC               GGTCTC(1/5)
             2504  GAGACC              
BseRI        1431  CTCCTC               GAGGAG(10/8)
             1069  GAGGAG              
BspHI        1323  TCATGA               T^CATGA
             2399  TCATGA              
BstUI        1466  CGCG                 CG^CG
             2606  CGCG                
Bsu36I        454  CCTGAGG              CC^T[ACGT]AGG
             1102  CCTGAGG             
Eco47III      632  AGCGCT               AGC^GCT
             1682  AGCGCT              
Eco81I        454  CCTGAGG              CC^T[ACGT]AGG
             1102  CCTGAGG             
EcoNI          87  CCTGAAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              851  CCTCCCACAGG         
HgaI         1119  GCGTC                GACGC(5/10)
             1290  GCGTC               
MaeI         2731  CTAG                 C^TAG
             2785  CTAG                
MseI         1205  TTAA                 T^TAA
             2162  TTAA                
MvnI         1466  CGCG                 CG^CG
             2606  CGCG                
NspI          471  ACATGC               [GA]CATG^[CT]
              740  ACATGT              
RcaI         1323  TCATGA               T^CATGA
             2399  TCATGA              
SmaI          443  CCCGGG               CCC^GGG
             1655  CCCGGG              
Tru9I        1205  TTAA                 T^TAA
             2162  TTAA                
XcmI          645  CCACCGTGTTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              701  CCAAGAGGTGGCTGG     
XmaCI         443  CCCGGG               C^CCGGG
             1655  CCCGGG              
XmaI          443  CCCGGG               C^CCGGG
             1655  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AciI        AfaI        AhdI        AluI        AlwI        
AlwNI       ApaI        AspEI       AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbvI        
BcnI        BmyI        BpmI        BsaJI       BsaWI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
Eam1105I    EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PpuMI       PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       
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