Restriction map for pF1KB8432
 (Linear) (Six-base) MAPSORT of: KIBB8432.seq  Check: 2827  from: 1  to: 2799



KIBB8432.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     673    2799 
   Size:        673    2126
 
AccI GT'mk_AC

Cuts at:      0    1753    2030    2799 
   Size:       1753     277     769

  Fragments arranged by size: 

                1753     769     277
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     130    1135    2017    2799 
   Size:        109      21    1005     882     782

  Fragments arranged by size: 

                1005     882     782     109      21
 
AfeI AGC'GCT

Cuts at:      0     634    1684    2799 
   Size:        634    1050    1115

  Fragments arranged by size: 

                1115    1050     634
 
AflIII A'CryG_T

Cuts at:      0     740    2605    2799 
   Size:        740    1865     194

  Fragments arranged by size: 

                1865     740     194
 
AhdI GACnn_n'nnGTC

Cuts at:      0     352    1836    2129    2799 
   Size:        352    1484     293     670

  Fragments arranged by size: 

                1484     670     352     293
 
AleI CACnn'nnGTG

Cuts at:      0     900    2799 
   Size:        900    1899
 
AlwNI CAG_nnn'CTG

Cuts at:      0     153     327     454    2799 
   Size:        153     174     127    2345

  Fragments arranged by size: 

                2345     174     153     127
 
ApaI G_GGCC'C

Cuts at:      0     909     937    1320    2799 
   Size:        909      28     383    1479

  Fragments arranged by size: 

                1479     909     383      28
 
ApaLI G'TGCA_C

Cuts at:      0     160    1800    2799 
   Size:        160    1640     999

  Fragments arranged by size: 

                1640     999     160
 
AvaI C'yCGr_G

Cuts at:      0     407     443     748    1655    2799 
   Size:        407      36     305     907    1144

  Fragments arranged by size: 

                1144     907     407     305      36
 
BamHI G'GATC_C

Cuts at:      0     232    2799 
   Size:        232    2567
 
BanI G'GyrC_C

Cuts at:      0     211     757    2237    2799 
   Size:        211     546    1480     562

  Fragments arranged by size: 

                1480     562     546     211
 
BanII G_rGCy'C

Cuts at:      0     463     909     937    1065    1320    2255    2648    2687 
   Size:        463     446      28     128     255     935     393      39

Cuts at:   2687    2799 
   Size:        112

  Fragments arranged by size: 

                 935     463     446     393     255     128     112      39
                  28
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     739     773    2799 
   Size:        739      34    2026

  Fragments arranged by size: 

                2026     739      34
 
BglII A'GATC_T

Cuts at:      0    2576    2799 
   Size:       2576     223
 
Bme1580I G_kGCm'C

Cuts at:      0     164     214     909     937    1320    1804    2240    2799 
   Size:        164      50     695      28     383     484     436     559

  Fragments arranged by size: 

                 695     559     484     436     383     164      50      28
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      41    1563    2799 
   Size:         41    1522    1236

  Fragments arranged by size: 

                1522    1236      41
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     450    2799 
   Size:        450    2349
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33      43     386     517     634    1161    1873    2799 
   Size:         33      10     343     131     117     527     712     926

  Fragments arranged by size: 

                 926     712     527     343     131     117      33      10
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     636    2799 
   Size:        636    2163
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     856    1049    1144    2457    2707    2799 
   Size:        856     193      95    1313     250      92

  Fragments arranged by size: 

                1313     856     250     193      95      92
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     671    2498    2799 
   Size:        671    1827     301

  Fragments arranged by size: 

                1827     671     301
 
BsaAI yAC'GTr

Cuts at:      0    2034    2634    2799 
   Size:       2034     600     165

  Fragments arranged by size: 

                2034     600     165
 
BsaBI GATnn'nnATC

Cuts at:      0    1078    2799 
   Size:       1078    1721
 
BsaWI w'CCGG_w

Cuts at:      0     113     626    2291    2327    2799 
   Size:        113     513    1665      36     472

  Fragments arranged by size: 

                1665     513     472     113      36
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1084    1422    2799 
   Size:       1084     338    1377

  Fragments arranged by size: 

                1377    1084     338
 
BseYI C'CCAG_C

Cuts at:      0     105    1391    1451    1518    2229    2337    2757    2799 
   Size:        105    1286      60      67     711     108     420      42

  Fragments arranged by size: 

                1286     711     420     108     105      67      60      42
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     234     897     943    1252    1387    1579    1864    2092 
   Size:        234     663      46     309     135     192     285     228

Cuts at:   2092    2799 
   Size:        707

  Fragments arranged by size: 

                 707     663     309     285     234     228     192     135
                  46
 
BsiEI CG_ry'CG

Cuts at:      0     114    2799 
   Size:        114    2685
 
BsiHKAI G_wGCw'C

Cuts at:      0     164     820    1065    1804    2255    2279    2687    2799 
   Size:        164     656     245     739     451      24     408     112

  Fragments arranged by size: 

                 739     656     451     408     245     164     112      24
            
 
BsmI GAATG_Cn'

Cuts at:      0    1357    2799 
   Size:       1357    1442
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1591    2250    2377    2799 
   Size:       1591     659     127     422

  Fragments arranged by size: 

                1591     659     422     127
 
Bsp1286I G_dGCh'C

Cuts at:      0     164     214     463     820     909     937    1065    1320 
   Size:        164      50     249     357      89      28     128     255

Cuts at:   1320    1804    2240    2255    2279    2648    2687    2799 
   Size:        484     436      15      24     369      39     112

  Fragments arranged by size: 

                 484     436     369     357     255     249     164     128
                 112      89      50      39      28      24      15
 
BspEI T'CCGG_A

Cuts at:      0     626    2799 
   Size:        626    2173
 
BspHI T'CATG_A

Cuts at:      0    1323    2399    2799 
   Size:       1323    1076     400

  Fragments arranged by size: 

                1323    1076     400
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     256     439     673    1015    1409    1748    1886    2417 
   Size:        256     183     234     342     394     339     138     531

Cuts at:   2417    2799 
   Size:        382

  Fragments arranged by size: 

                 531     394     382     342     339     256     234     183
                 138
 
BsrFI r'CCGG_y

Cuts at:      0     887     962    1568    1650    2799 
   Size:        887      75     606      82    1149

  Fragments arranged by size: 

                1149     887     606      82      75
 
BsrGI T'GTAC_A

Cuts at:      0     737    2799 
   Size:        737    2062
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     837    2799 
   Size:        837    1962
 
BstBI TT'CG_AA

Cuts at:      0    1577    2799 
   Size:       1577    1222
 
BstYI r'GATC_y

Cuts at:      0     232    2496    2576    2799 
   Size:        232    2264      80     223

  Fragments arranged by size: 

                2264     232     223      80
 
Bsu36I CC'TnA_GG

Cuts at:      0     455    1103    2799 
   Size:        455     648    1696

  Fragments arranged by size: 

                1696     648     455
 
BtgI C'CryG_G

Cuts at:      0    1615    1641    2075    2171    2639    2654    2799 
   Size:       1615      26     434      96     468      15     145

  Fragments arranged by size: 

                1615     468     434     145      96      26      15
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1988    2140    2265    2799 
   Size:       1988     152     125     534

  Fragments arranged by size: 

                1988     534     152     125
 
BtsI GCAGTG_nn'

Cuts at:      0    1804    2451    2799 
   Size:       1804     647     348

  Fragments arranged by size: 

                1804     647     348
 
ClaI AT'CG_AT

Cuts at:      0     845    2799 
   Size:        845    1954
 
DraI TTT'AAA

Cuts at:      0    1206    2799 
   Size:       1206    1593
 
DraIII CAC_nnn'GTG

Cuts at:      0     837    2799 
   Size:        837    1962
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2296    2799 
   Size:       2296     503
 
EaeI y'GGCC_r

Cuts at:      0     131     642     672    1386    1763    2360    2485    2636 
   Size:        131     511      30     714     377     597     125     151

Cuts at:   2636    2651    2799 
   Size:         15     148

  Fragments arranged by size: 

                 714     597     511     377     151     148     131     125
                  30      15
 
EarI CTCTTCn'nnn_

Cuts at:      0      28    2799 
   Size:         28    2771
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1186    1965    2799 
   Size:       1186     779     834

  Fragments arranged by size: 

                1186     834     779
 
EcoICRI GAG'CTC

Cuts at:      0    1063    2253    2685    2799 
   Size:       1063    1190     432     114

  Fragments arranged by size: 

                1190    1063     432     114
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33      43     109     130     386     517     634    1135 
   Size:         33      10      66      21     256     131     117     501

Cuts at:   1135    1161    1873    2017    2799 
   Size:         26     712     144     782

  Fragments arranged by size: 

                 782     712     501     256     144     131     117      66
                  33      26      21      10
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      91     855    2799 
   Size:         91     764    1944

  Fragments arranged by size: 

                1944     764      91
 
EcoO109I rG'GnC_Cy

Cuts at:      0     102     186     336     451     860     933     995    1659 
   Size:        102      84     150     115     409      73      62     664

Cuts at:   1659    2123    2799 
   Size:        464     676

  Fragments arranged by size: 

                 676     664     464     409     150     115     102      84
                  73      62
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     744    2472    2799 
   Size:        744    1728     327

  Fragments arranged by size: 

                1728     744     327
 
HaeII r_GCGC'y

Cuts at:      0     636    1258    1686    2799 
   Size:        636     622     428    1113

  Fragments arranged by size: 

                1113     636     622     428
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     133     165     338     370     420     452    1590    1622 
   Size:        133      32     173      32      50      32    1138      32

Cuts at:   1622    1822    1854    2115    2147    2495    2527    2725    2757 
   Size:        200      32     261      32     348      32     198      32

Cuts at:   2757    2799 
   Size:         42

  Fragments arranged by size: 

                1138     348     261     200     198     173     133      50
                  42      32      32      32      32      32      32      32
                  32
 
MluI A'CGCG_T

Cuts at:      0    2605    2799 
   Size:       2605     194
 
MscI TGG'CCA

Cuts at:      0     133     644    2653    2799 
   Size:        133     511    2009     146

  Fragments arranged by size: 

                2009     511     146     133
 
MslI CAynn'nnrTG

Cuts at:      0     900     965    1133    1282    2799 
   Size:        900      65     168     149    1517

  Fragments arranged by size: 

                1517     900     168     149      65
 
MspA1I CmG'CkG

Cuts at:      0     230     579     957    1391    1444    1563    1835    2585 
   Size:        230     349     378     434      53     119     272     750

Cuts at:   2585    2757    2799 
   Size:        172      42

  Fragments arranged by size: 

                 750     434     378     349     272     230     172     119
                  53      42
 
NcoI C'CATG_G

Cuts at:      0    1615    1641    2171    2799 
   Size:       1615      26     530     628

  Fragments arranged by size: 

                1615     628     530      26
 
NspI r_CATG'y

Cuts at:      0     475     744    2799 
   Size:        475     269    2055

  Fragments arranged by size: 

                2055     475     269
 
PciI A'CATG_T

Cuts at:      0     740    2799 
   Size:        740    2059
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     207     707    1553    2799 
   Size:        207     500     846    1246

  Fragments arranged by size: 

                1246     846     500     207
 
PfoI T'CCnGG_A

Cuts at:      0     289     563     590    1788    2624    2799 
   Size:        289     274      27    1198     836     175

  Fragments arranged by size: 

                1198     836     289     274     175      27
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     237     269    1909    1941    2381    2413    2799 
   Size:        237      32    1640      32     440      32     386

  Fragments arranged by size: 

                1640     440     386     237      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     102     186     336     451     860     995    1659    2123 
   Size:        102      84     150     115     409     135     664     464

Cuts at:   2123    2799 
   Size:        676

  Fragments arranged by size: 

                 676     664     464     409     150     135     115     102
                  84
 
PspOMI G'GGCC_C

Cuts at:      0     905     933    1316    2799 
   Size:        905      28     383    1483

  Fragments arranged by size: 

                1483     905     383      28
 
PvuII CAG'CTG

Cuts at:      0     579     957    1444    1563    2799 
   Size:        579     378     487     119    1236

  Fragments arranged by size: 

                1236     579     487     378     119
 
SacI G_AGCT'C

Cuts at:      0    1065    2255    2687    2799 
   Size:       1065    1190     432     112

  Fragments arranged by size: 

                1190    1065     432     112
 
SanDI GG'GwC_CC

Cuts at:      0     186    2799 
   Size:        186    2613
 
SexAI A'CCwGG_T

Cuts at:      0    2705    2799 
   Size:       2705      94
 
SfcI C'TryA_G

Cuts at:      0     952    1242    1530    2799 
   Size:        952     290     288    1269

  Fragments arranged by size: 

                1269     952     290     288
 
SmaI CCC'GGG

Cuts at:      0     445    1657    2799 
   Size:        445    1212    1142

  Fragments arranged by size: 

                1212    1142     445
 
SmlI C'TyrA_G

Cuts at:      0     748     871    1064    1159    2436    2722    2799 
   Size:        748     123     193      95    1277     286      77

  Fragments arranged by size: 

                1277     748     286     193     123      95      77
 
StyI C'CwwG_G

Cuts at:      0     167     236     998    1223    1422    1547    1615    1641 
   Size:        167      69     762     225     199     125      68      26

Cuts at:   1641    1706    2171    2648    2669    2799 
   Size:         65     465     477      21     130

  Fragments arranged by size: 

                 762     477     465     225     199     167     130     125
                  69      68      65      26      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1310    1545    2666    2704    2799 
   Size:       1310     235    1121      38      95

  Fragments arranged by size: 

                1310    1121     235      95      38
 
TatI w'GTAC_w

Cuts at:      0     737    1715    2799 
   Size:        737     978    1084

  Fragments arranged by size: 

                1084     978     737
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     170     202     224     256    2646    2678    2799 
   Size:        170      32      22      32    2390      32     121

  Fragments arranged by size: 

                2390     170     121      32      32      32      22
 
Tth111I GACn'n_nGTC

Cuts at:      0     525    2799 
   Size:        525    2274
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     652     708    2799 
   Size:        652      56    2091

  Fragments arranged by size: 

                2091     652      56
 
XhoI C'TCGA_G

Cuts at:      0     748    2799 
   Size:        748    2051
 
XmaI C'CCGG_G

Cuts at:      0     443    1655    2799 
   Size:        443    1212    1144

  Fragments arranged by size: 

                1212    1144     443
 
XmnI GAAnn'nnTTC

Cuts at:      0    1924    2799 
   Size:       1924     875
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AhdI     AleI    AlwNI
     ApaI    ApaLI     AvaI    BamHI     BanI    BanII     BcgI    BglII
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrFI    BsrGI   BstAPI
    BstBI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI
   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I     MluI     MscI     MslI   MspA1I
     NcoI     NspI     PciI    PflMI     PfoI     PpiI    PpuMI   PspOMI
    PvuII     SacI    SanDI    SexAI     SfcI     SmaI     SmlI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AlfI     AloI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BbsI    BbvCI
    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BmtI    BsaHI
    BsaXI    BsiWI    BsrBI    BsrDI   BssHII    BssSI   BstEII    BstXI
  BstZ17I    CspCI     EagI    EcoRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MmeI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    RsrII    SacII     SalI     SapI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     XbaI     ZraI
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