Restriction map for
pF1KB8429
(Circular) (Six-base) MAPSORT of: pF1KB8429.seq Check: 4704 from: 1 to: 4418
pF1KB8429 4418 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1134 1134
Size: 4418
AatII G_ACGT'C
Cuts at: 1079 1079
Size: 4418
AccI GT'mk_AC
Cuts at: 979 1456 979
Size: 477 3941
Acc65I G'GTAC_C
Cuts at: 1302 1434 1302
Size: 132 4286
AclI AA'CG_TT
Cuts at: 4258 4258
Size: 4418
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 537 721 817 2243 2675 3028 3690 322
Size: 215 184 96 1426 432 353 662 1050
Fragments arranged by size:
1426 1050 662 432 353 215 184 96
AfeI AGC'GCT
Cuts at: 1399 1399
Size: 4418
AflIII A'CryG_T
Cuts at: 875 1770 3570 3851 875
Size: 895 1800 281 1442
Fragments arranged by size:
1800 1442 895 281
AgeI A'CCGG_T
Cuts at: 240 1332 240
Size: 1092 3326
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 388 388
Size: 4418
AlwNI CAG_nnn'CTG
Cuts at: 1062 1119 1254 1257 1723 3161 1062
Size: 57 135 3 466 1438 2319
Fragments arranged by size:
2319 1438 466 135 57 3
ApaI G_GGCC'C
Cuts at: 885 885
Size: 4418
ApaLI G'TGCA_C
Cuts at: 427 688 3256 427
Size: 261 2568 1589
Fragments arranged by size:
2568 1589 261
ApoI r'AATT_y
Cuts at: 1422 3771 4027 1422
Size: 2349 256 1813
Fragments arranged by size:
2349 1813 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4418
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4418
AvaI C'yCGr_G
Cuts at: 763 1438 763
Size: 675 3743
BamHI G'GATC_C
Cuts at: 1443 1443
Size: 4418
BanI G'GyrC_C
Cuts at: 243 1302 1434 1848 2096 2131 243
Size: 1059 132 414 248 35 2530
Fragments arranged by size:
2530 1059 414 248 132 35
BanII G_rGCy'C
Cuts at: 817 885 953 1279 1432 2462 817
Size: 68 68 326 153 1030 2773
Fragments arranged by size:
2773 1030 326 153 68 68
BbeI G_GCGC'C
Cuts at: 1852 2100 1852
Size: 248 4170
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 504 538 504
Size: 34 4384
BciVI GTATCCnnnnn_n'
Cuts at: 2316 3372 4418 2316
Size: 1056 1046 2316
Fragments arranged by size:
2316 1056 1046
BclI T'GATC_A
Cuts at: 1938 1938
Size: 4418
BglI GCCn_nnn'nGGC
Cuts at: 1390 1390
Size: 4418
BglII A'GATC_T
Cuts at: 1933 3970 1933
Size: 2037 2381
BlpI GC'TnA_GC
Cuts at: 613 841 1530 613
Size: 228 689 3501
Fragments arranged by size:
3501 689 228
Bme1580I G_kGCm'C
Cuts at: 431 692 885 2043 2136 3260 431
Size: 261 193 1158 93 1124 1589
Fragments arranged by size:
1589 1158 1124 261 193 93
BmgBI CAC'GTC
Cuts at: 648 837 648
Size: 189 4229
BmrI ACTGGGnnnn_n'
Cuts at: 863 1215 1630 1711 2046 4190 863
Size: 352 415 81 335 2144 1091
Fragments arranged by size:
2144 1091 415 352 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1233 1293 2785 1233
Size: 60 1492 2866
Fragments arranged by size:
2866 1492 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 1102 337
Size: 765 3653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1594 2473 3187 3485 1594
Size: 879 714 298 2527
Fragments arranged by size:
2527 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 689 4122 689
Size: 3433 985
BsaAI yAC'GTr
Cuts at: 1771 1771
Size: 4418
BsaBI GATnn'nnATC
Cuts at: 6 745 1932 3969 6
Size: 739 1187 2037 455
Fragments arranged by size:
2037 1187 739 455
BsaHI Gr'CG_yC
Cuts at: 1076 1289 1849 2097 2799 4295 1076
Size: 213 560 248 702 1496 1199
Fragments arranged by size:
1496 1199 702 560 248 213
BsaWI w'CCGG_w
Cuts at: 240 1028 1332 1607 2128 2806 3217 3364 3638
Size: 788 304 275 521 678 411 147 274
Cuts at: 3638 240
Size: 1020
Fragments arranged by size:
1020 788 678 521 411 304 275 274
147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 146 334 1225 1282 146
Size: 188 891 57 3282
Fragments arranged by size:
3282 891 188 57
BseYI C'CCAG_C
Cuts at: 136 705 1052 1298 1344 1843 2601 3266 3674
Size: 569 347 246 46 499 758 665 408
Cuts at: 3674 136
Size: 880
Fragments arranged by size:
880 758 665 569 499 408 347 246
46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 361 391 655 277
Size: 84 30 264 4040
Fragments arranged by size:
4040 264 84 30
BsiEI CG_ry'CG
Cuts at: 104 849 853 1960 2006 3236 104
Size: 745 4 1107 46 1230 1286
Fragments arranged by size:
1286 1230 1107 745 46 4
BsiHKAI G_wGCw'C
Cuts at: 431 546 692 722 842 899 953 1219 1279
Size: 115 146 30 120 57 54 266 60
Cuts at: 1279 1432 2210 2400 3260 431
Size: 153 778 190 860 1589
Fragments arranged by size:
1589 860 778 266 190 153 146 120
115 60 57 54 30
Bsp1286I G_dGCh'C
Cuts at: 431 546 692 722 817 842 885 899 953
Size: 115 146 30 95 25 43 14 54
Cuts at: 953 1219 1279 1432 2043 2136 2210 2400 2462
Size: 266 60 153 611 93 74 190 62
Cuts at: 2462 3260 431
Size: 798 1589
Fragments arranged by size:
1589 798 611 266 190 153 146 115
95 93 74 62 60 54 43 30
25 14
BspEI T'CCGG_A
Cuts at: 1028 1028
Size: 4418
BspHI T'CATG_A
Cuts at: 546 2850 546
Size: 2304 2114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 590 800 1134 1412 1468 1682 1984 2365 590
Size: 210 334 278 56 214 302 381 2643
Fragments arranged by size:
2643 381 334 302 278 214 210 56
BsrBI CCG'CTC
Cuts at: 50 357 2710 2764 4248 4413 50
Size: 307 2353 54 1484 165 55
Fragments arranged by size:
2353 1484 307 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1100 1182 2330 1100
Size: 82 1148 3188
Fragments arranged by size:
3188 1148 82
BsrFI r'CCGG_y
Cuts at: 240 848 1332 2416 2597 240
Size: 608 484 1084 181 2061
Fragments arranged by size:
2061 1084 608 484 181
BssSI C'ACGA_G
Cuts at: 2689 3397 2689
Size: 708 3710
BstAPI GCAn_nnn'nTGC
Cuts at: 1257 1795 1257
Size: 538 3880
BstBI TT'CG_AA
Cuts at: 2779 2779
Size: 4418
BstXI CCAn_nnnn'nTGG
Cuts at: 165 492 165
Size: 327 4091
BstYI r'GATC_y
Cuts at: 1 305 1443 1910 1933 2268 2514 2820 2832
Size: 304 1138 467 23 335 246 306 12
Cuts at: 2832 2918 3970 1
Size: 86 1052 449
Fragments arranged by size:
1138 1052 467 449 335 306 304 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 980 980
Size: 4418
Bsu36I CC'TnA_GG
Cuts at: 989 989
Size: 4418
BtgI C'CryG_G
Cuts at: 435 858 435
Size: 423 3995
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2368 2549 2712 2368
Size: 181 163 4074
Fragments arranged by size:
4074 181 163
BtsI GCAGTG_nn'
Cuts at: 265 391 1661 265
Size: 126 1270 3022
Fragments arranged by size:
3022 1270 126
DraI TTT'AAA
Cuts at: 1417 1417
Size: 4418
DraIII CAC_nnn'GTG
Cuts at: 1019 1019
Size: 4418
DrdI GACnn_nn'nnGTC
Cuts at: 2124 3468 2124
Size: 1344 3074
EaeI y'GGCC_r
Cuts at: 149 846 850 915 1064 1262 1382 2003 2177
Size: 697 4 65 149 198 120 621 174
Cuts at: 2177 2568 2595 3940 149
Size: 391 27 1345 627
Fragments arranged by size:
1345 697 627 621 391 198 174 149
120 65 27 4
EagI C'GGCC_G
Cuts at: 846 850 2003 846
Size: 4 1153 3261
Fragments arranged by size:
3261 1153 4
EarI CTCTTCn'nnn_
Cuts at: 347 448 601 904 958 1267 2441 2651 347
Size: 101 153 303 54 309 1174 210 2114
Fragments arranged by size:
2114 1174 309 303 210 153 101 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2626 3368 3514 4228 2626
Size: 742 146 714 2816
Fragments arranged by size:
2816 742 714 146
EcoICRI GAG'CTC
Cuts at: 951 1277 1430 951
Size: 326 153 3939
Fragments arranged by size:
3939 326 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 337 537 721 817 1102 2243 2675 3028
Size: 15 200 184 96 285 1141 432 353
Cuts at: 3028 3690 322
Size: 662 1050
Fragments arranged by size:
1141 1050 662 432 353 285 200 184
96 15
EcoNI CCTnn'n_nnAGG
Cuts at: 445 796 445
Size: 351 4067
EcoO109I rG'GnC_Cy
Cuts at: 666 789 882 1086 1557 666
Size: 123 93 204 471 3527
Fragments arranged by size:
3527 471 204 123 93
EcoRI G'AATT_C
Cuts at: 1422 1422
Size: 4418
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2437 2437
Size: 4418
FseI GG_CCGG'CC
Cuts at: 852 852
Size: 4418
FspI TGC'GCA
Cuts at: 2199 2199
Size: 4418
HaeII r_GCGC'y
Cuts at: 1062 1401 1852 2100 3330 4101 1062
Size: 339 451 248 1230 771 1379
Fragments arranged by size:
1379 1230 771 451 339 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 197 229 466 498 720 752 2260 2290 2292
Size: 32 237 32 222 32 1508 30 2
Cuts at: 2292 2322 197
Size: 30 2293
Fragments arranged by size:
2293 1508 237 222 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1457 1457
Size: 4418
KasI G'GCGC_C
Cuts at: 1848 2096 1848
Size: 248 4170
KpnI G_GTAC'C
Cuts at: 1306 1438 1306
Size: 132 4286
MluI A'CGCG_T
Cuts at: 3851 3851
Size: 4418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3177 3361 3177
Size: 184 4234
MscI TGG'CCA
Cuts at: 917 2179 917
Size: 1262 3156
MslI CAynn'nnrTG
Cuts at: 2407 2534 2407
Size: 127 4291
MspA1I CmG'CkG
Cuts at: 300 568 845 1254 1529 1843 2203 2987 3232
Size: 268 277 409 275 314 360 784 245
Cuts at: 3232 300
Size: 1486
Fragments arranged by size:
1486 784 409 360 314 277 275 268
245
NaeI GCC'GGC
Cuts at: 850 2599 850
Size: 1749 2669
NarI GG'CG_CC
Cuts at: 1849 2097 1849
Size: 248 4170
NgoMIV G'CCGG_C
Cuts at: 848 2597 848
Size: 1749 2669
NspI r_CATG'y
Cuts at: 879 1245 1471 1971 3574 879
Size: 366 226 500 1603 1723
Fragments arranged by size:
1723 1603 500 366 226
PasI CC'CwG_GG
Cuts at: 885 885
Size: 4418
PciI A'CATG_T
Cuts at: 875 3570 875
Size: 2695 1723
PfoI T'CCnGG_A
Cuts at: 446 527 1079 446
Size: 81 552 3785
Fragments arranged by size:
3785 552 81
PmeI GTTT'AAAC
Cuts at: 1417 1417
Size: 4418
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2864 2896 2864
Size: 32 4386
PpuMI rG'GwC_Cy
Cuts at: 789 789
Size: 4418
PspOMI G'GGCC_C
Cuts at: 881 881
Size: 4418
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 904 936 904
Size: 32 4386
PstI C_TGCA'G
Cuts at: 587 1465 2150 587
Size: 878 685 2855
Fragments arranged by size:
2855 878 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4418
PvuII CAG'CTG
Cuts at: 300 1254 1843 2203 300
Size: 954 589 360 2515
Fragments arranged by size:
2515 954 589 360
RsrII CG'GwC_CG
Cuts at: 2613 2613
Size: 4418
SacI G_AGCT'C
Cuts at: 953 1279 1432 953
Size: 326 153 3939
Fragments arranged by size:
3939 326 153
SalI G'TCGA_C
Cuts at: 1455 1455
Size: 4418
SapI GCTCTTCn'nnn_
Cuts at: 347 904 958 1267 2441 2651 347
Size: 557 54 309 1174 210 2114
Fragments arranged by size:
2114 1174 557 309 210 54
SbfI CC_TGCA'GG
Cuts at: 1465 1465
Size: 4418
ScaI AGT'ACT
Cuts at: 388 3935 388
Size: 3547 871
SfcI C'TryA_G
Cuts at: 33 583 1461 2146 3114 3305 33
Size: 550 878 685 968 191 1146
Fragments arranged by size:
1146 968 878 685 550 191
SfoI GGC'GCC
Cuts at: 1850 2098 1850
Size: 248 4170
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4418
SgrAI Cr'CCGG_yG
Cuts at: 240 1332 240
Size: 1092 3326
SmaI CCC'GGG
Cuts at: 1440 1440
Size: 4418
SmlI C'TyrA_G
Cuts at: 1573 2488 3202 3464 1573
Size: 915 714 262 2527
Fragments arranged by size:
2527 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4418
SphI G_CATG'C
Cuts at: 1471 1971 1471
Size: 500 3918
StyI C'CwwG_G
Cuts at: 158 216 333 1373 1552 1906 158
Size: 58 117 1040 179 354 2670
Fragments arranged by size:
2670 1040 354 179 117 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2001 2134 2593 2802 2001
Size: 133 459 209 3617
Fragments arranged by size:
3617 459 209 133
TatI w'GTAC_w
Cuts at: 386 3933 386
Size: 3547 871
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3935 3967 3935
Size: 32 4386
Tth111I GACn'n_nGTC
Cuts at: 649 2215 649
Size: 1566 2852
XbaI T'CTAG_A
Cuts at: 1449 1449
Size: 4418
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1371 1913 1371
Size: 542 3876
XmaI C'CCGG_G
Cuts at: 1438 1438
Size: 4418
ZraI GAC'GTC
Cuts at: 1077 1077
Size: 4418
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AloI AscI AvrII BaeI BbsI
BbvCI BfrBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI
BsrGI BssHII BstEII ClaI CspCI EcoRV FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PshAI PsiI PspXI SacII SanDI
SexAI SfiI SnaBI SrfI SspI StuI SwaI XhoI
XmnI