Restriction map for
pF1KB8427
(Circular) (Six-base) MAPSORT of: pF1KB8427.seq Check: 6985 from: 1 to: 5828
pF1KB8427 5828 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 362 2492 2866 362
Size: 2130 374 3324
Fragments arranged by size:
3324 2130 374
Acc65I G'GTAC_C
Cuts at: 2844 2844
Size: 5828
AclI AA'CG_TT
Cuts at: 5668 5668
Size: 5828
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 590 1282 1603 1903 2206 2291 2296 2710
Size: 220 692 321 300 303 85 5 414
Cuts at: 2710 3653 4085 4438 5100 370
Size: 943 432 353 662 1098
Fragments arranged by size:
1098 943 692 662 432 414 353 321
303 300 220 85 5
AflII C'TTAA_G
Cuts at: 327 892 327
Size: 565 5263
AflIII A'CryG_T
Cuts at: 2359 3180 4980 5261 2359
Size: 821 1800 281 2926
Fragments arranged by size:
2926 1800 821 281
AhdI GACnn_n'nnGTC
Cuts at: 149 149
Size: 5828
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2734 2766 2734
Size: 32 5796
AlwNI CAG_nnn'CTG
Cuts at: 1513 2806 3133 4571 1513
Size: 1293 327 1438 2770
Fragments arranged by size:
2770 1438 1293 327
ApaLI G'TGCA_C
Cuts at: 4666 4666
Size: 5828
ApoI r'AATT_y
Cuts at: 503 2514 2832 5181 5437 503
Size: 2011 318 2349 256 894
Fragments arranged by size:
2349 2011 894 318 256
AseI AT'TA_AT
Cuts at: 20 535 1906 2446 20
Size: 515 1371 540 3402
Fragments arranged by size:
3402 1371 540 515
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5828
AvaI C'yCGr_G
Cuts at: 2848 2848
Size: 5828
BamHI G'GATC_C
Cuts at: 981 2853 981
Size: 1872 3956
BanI G'GyrC_C
Cuts at: 517 2844 3258 3506 3541 517
Size: 2327 414 248 35 2804
Fragments arranged by size:
2804 2327 414 248 35
BanII G_rGCy'C
Cuts at: 2842 3872 2842
Size: 1030 4798
BbeI G_GCGC'C
Cuts at: 3262 3510 3262
Size: 248 5580
BbsI GAAGACnn'nnnn_
Cuts at: 305 376 940 958 305
Size: 71 564 18 5175
Fragments arranged by size:
5175 564 71 18
BciVI GTATCCnnnnn_n'
Cuts at: 3726 4782 5828 3726
Size: 1056 1046 3726
Fragments arranged by size:
3726 1056 1046
BclI T'GATC_A
Cuts at: 1290 3348 1290
Size: 2058 3770
BfrBI ATG'CAT
Cuts at: 1410 1410
Size: 5828
BglII A'GATC_T
Cuts at: 2031 3343 5380 2031
Size: 1312 2037 2479
Fragments arranged by size:
2479 2037 1312
BlpI GC'TnA_GC
Cuts at: 2084 2940 2084
Size: 856 4972
Bme1580I G_kGCm'C
Cuts at: 3453 3546 4670 3453
Size: 93 1124 4611
Fragments arranged by size:
4611 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 3040 3121 3456 5600 3040
Size: 81 335 2144 3268
Fragments arranged by size:
3268 2144 335 81
BmtI G_CTAG'C
Cuts at: 2512 2512
Size: 5828
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4195 4195
Size: 5828
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 294 2090 2398 3004 3883 4597 4895 294
Size: 1796 308 606 879 714 298 1227
Fragments arranged by size:
1796 1227 879 714 606 308 298
BsaI GGTCTCn'nnnn_
Cuts at: 5532 5532
Size: 5828
BsaAI yAC'GTr
Cuts at: 3181 3181
Size: 5828
BsaBI GATnn'nnATC
Cuts at: 6 3342 5379 6
Size: 3336 2037 455
Fragments arranged by size:
3336 2037 455
BsaHI Gr'CG_yC
Cuts at: 3259 3507 4209 5705 3259
Size: 248 702 1496 3382
Fragments arranged by size:
3382 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3017 3538 4216 4627 4774 5048 3017
Size: 521 678 411 147 274 3797
Fragments arranged by size:
3797 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2734 2764 2734
Size: 30 5798
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1853 1853
Size: 5828
BseYI C'CCAG_C
Cuts at: 3253 4011 4676 5084 3253
Size: 758 665 408 3997
Fragments arranged by size:
3997 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 3370 3416 4646 104
Size: 3266 46 1230 1286
Fragments arranged by size:
3266 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 935 2842 3620 3810 4670 935
Size: 1907 778 190 860 2093
Fragments arranged by size:
2093 1907 860 778 190
BsmI GAATG_Cn'
Cuts at: 274 467 274
Size: 193 5635
Bsp1286I G_dGCh'C
Cuts at: 935 2842 3453 3546 3620 3810 3872 4670 935
Size: 1907 611 93 74 190 62 798 2093
Fragments arranged by size:
2093 1907 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 4260 4260
Size: 5828
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2878 3092 3394 3775 2878
Size: 214 302 381 4931
Fragments arranged by size:
4931 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4120 4174 5658 5823 50
Size: 4070 54 1484 165 55
Fragments arranged by size:
4070 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2771 3740 2771
Size: 969 4859
BsrFI r'CCGG_y
Cuts at: 3826 4007 3826
Size: 181 5647
BssSI C'ACGA_G
Cuts at: 4099 4807 4099
Size: 708 5120
BstAPI GCAn_nnn'nTGC
Cuts at: 1768 3205 1768
Size: 1437 4391
BstBI TT'CG_AA
Cuts at: 4189 4189
Size: 5828
BstYI r'GATC_y
Cuts at: 1 885 981 2031 2853 3320 3343 3678 3924
Size: 884 96 1050 822 467 23 335 246
Cuts at: 3924 4230 4242 4328 5380 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 1050 884 822 467 449 335 306
246 96 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5828
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2074 3778 3959 4122 2074
Size: 1704 181 163 3780
Fragments arranged by size:
3780 1704 181 163
BtsI GCAGTG_nn'
Cuts at: 116 469 1717 3071 116
Size: 353 1248 1354 2873
Fragments arranged by size:
2873 1354 1248 353
DraI TTT'AAA
Cuts at: 2827 2827
Size: 5828
DrdI GACnn_nn'nnGTC
Cuts at: 3534 4878 3534
Size: 1344 4484
EaeI y'GGCC_r
Cuts at: 3413 3587 3978 4005 5350 3413
Size: 174 391 27 1345 3891
Fragments arranged by size:
3891 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3413 3413
Size: 5828
EarI CTCTTCn'nnn_
Cuts at: 758 999 2162 2696 3851 4061 758
Size: 241 1163 534 1155 210 2525
Fragments arranged by size:
2525 1163 1155 534 241 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4036 4778 4924 5638 4036
Size: 742 146 714 4226
Fragments arranged by size:
4226 742 714 146
EcoICRI GAG'CTC
Cuts at: 2840 2840
Size: 5828
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 590 1282 1603 1903 2206 2291 2296 2710
Size: 220 692 321 300 303 85 5 414
Cuts at: 2710 3653 4085 4438 5100 370
Size: 943 432 353 662 1098
Fragments arranged by size:
1098 943 692 662 432 414 353 321
303 300 220 85 5
EcoNI CCTnn'n_nnAGG
Cuts at: 593 2062 593
Size: 1469 4359
EcoO109I rG'GnC_Cy
Cuts at: 1623 2967 1623
Size: 1344 4484
EcoRI G'AATT_C
Cuts at: 2832 2832
Size: 5828
EcoRV GAT'ATC
Cuts at: 2598 2598
Size: 5828
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1175 3847 1175
Size: 2672 3156
FspI TGC'GCA
Cuts at: 3609 3609
Size: 5828
HaeII r_GCGC'y
Cuts at: 1094 3262 3510 4740 5511 1094
Size: 2168 248 1230 771 1411
Fragments arranged by size:
2168 1411 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 135 167 212 244 2695 2727 3670 3700 3702
Size: 32 45 32 2451 32 943 30 2
Cuts at: 3702 3732 135
Size: 30 2231
Fragments arranged by size:
2451 2231 943 45 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 488 873 1379 2867 488
Size: 385 506 1488 3449
Fragments arranged by size:
3449 1488 506 385
HindIII A'AGCT_T
Cuts at: 1602 1602
Size: 5828
HpaI GTT'AAC
Cuts at: 488 1379 488
Size: 891 4937
KasI G'GCGC_C
Cuts at: 3258 3506 3258
Size: 248 5580
KpnI G_GTAC'C
Cuts at: 2848 2848
Size: 5828
MluI A'CGCG_T
Cuts at: 5261 5261
Size: 5828
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 412 1864 4587 4771 412
Size: 1452 2723 184 1469
Fragments arranged by size:
2723 1469 1452 184
MscI TGG'CCA
Cuts at: 3589 3589
Size: 5828
MslI CAynn'nnrTG
Cuts at: 1060 3817 3944 1060
Size: 2757 127 2944
Fragments arranged by size:
2944 2757 127
MspA1I CmG'CkG
Cuts at: 1041 2939 3253 3613 4397 4642 1041
Size: 1898 314 360 784 245 2227
Fragments arranged by size:
2227 1898 784 360 314 245
NaeI GCC'GGC
Cuts at: 4009 4009
Size: 5828
NarI GG'CG_CC
Cuts at: 3259 3507 3259
Size: 248 5580
NcoI C'CATG_G
Cuts at: 107 107
Size: 5828
NdeI CA'TA_TG
Cuts at: 1049 1470 1049
Size: 421 5407
NgoMIV G'CCGG_C
Cuts at: 4007 4007
Size: 5828
NheI G'CTAG_C
Cuts at: 2508 2508
Size: 5828
NsiI A_TGCA'T
Cuts at: 1412 1412
Size: 5828
NspI r_CATG'y
Cuts at: 2363 2881 3381 4984 2363
Size: 518 500 1603 3207
Fragments arranged by size:
3207 1603 518 500
PciI A'CATG_T
Cuts at: 2359 4980 2359
Size: 2621 3207
PflMI CCAn_nnn'nTGG
Cuts at: 1161 1161
Size: 5828
PfoI T'CCnGG_A
Cuts at: 1884 1884
Size: 5828
PmeI GTTT'AAAC
Cuts at: 2827 2827
Size: 5828
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2766 4274 4306 2734
Size: 32 1508 32 4256
Fragments arranged by size:
4256 1508 32 32
PpuMI rG'GwC_Cy
Cuts at: 1623 1623
Size: 5828
PshAI GACnn'nnGTC
Cuts at: 1432 1432
Size: 5828
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2267 2299 2267
Size: 32 5796
PstI C_TGCA'G
Cuts at: 2875 3560 2875
Size: 685 5143
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5828
PvuII CAG'CTG
Cuts at: 1041 3253 3613 1041
Size: 2212 360 3256
Fragments arranged by size:
3256 2212 360
RsrII CG'GwC_CG
Cuts at: 4023 4023
Size: 5828
SacI G_AGCT'C
Cuts at: 2842 2842
Size: 5828
SalI G'TCGA_C
Cuts at: 2865 2865
Size: 5828
SapI GCTCTTCn'nnn_
Cuts at: 2162 3851 4061 2162
Size: 1689 210 3929
Fragments arranged by size:
3929 1689 210
SbfI CC_TGCA'GG
Cuts at: 2875 2875
Size: 5828
ScaI AGT'ACT
Cuts at: 1742 2328 5345 1742
Size: 586 3017 2225
Fragments arranged by size:
3017 2225 586
SfcI C'TryA_G
Cuts at: 33 478 1773 2871 3556 4524 4715 33
Size: 445 1295 1098 685 968 191 1146
Fragments arranged by size:
1295 1146 1098 968 685 445 191
SfoI GGC'GCC
Cuts at: 3260 3508 3260
Size: 248 5580
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5828
SmaI CCC'GGG
Cuts at: 2850 2850
Size: 5828
SmlI C'TyrA_G
Cuts at: 273 327 892 2105 2413 2983 3898 4612 4874
Size: 54 565 1213 308 570 915 714 262
Cuts at: 4874 273
Size: 1227
Fragments arranged by size:
1227 1213 915 714 570 565 308 262
54
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5828
SphI G_CATG'C
Cuts at: 2881 3381 2881
Size: 500 5328
SspI AAT'ATT
Cuts at: 740 821 828 1214 1700 1896 740
Size: 81 7 386 486 196 4672
Fragments arranged by size:
4672 486 386 196 81 7
StyI C'CwwG_G
Cuts at: 107 1358 2714 2962 3316 107
Size: 1251 1356 248 354 2619
Fragments arranged by size:
2619 1356 1251 354 248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3411 3544 4003 4212 3411
Size: 133 459 209 5027
Fragments arranged by size:
5027 459 209 133
TatI w'GTAC_w
Cuts at: 155 1680 1740 2326 2475 2535 5343 155
Size: 1525 60 586 149 60 2808 640
Fragments arranged by size:
2808 1525 640 586 149 60 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5345 5377 5345
Size: 32 5796
Tth111I GACn'n_nGTC
Cuts at: 3625 3625
Size: 5828
XbaI T'CTAG_A
Cuts at: 1317 2859 1317
Size: 1542 4286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2779 3323 2779
Size: 544 5284
XmaI C'CCGG_G
Cuts at: 2848 2848
Size: 5828
XmnI GAAnn'nnTTC
Cuts at: 864 1013 2309 864
Size: 149 1296 4383
Fragments arranged by size:
4383 1296 149
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NsiI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BpmI Bpu10I
BsgI BsiWI BsmBI BspEI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI MfeI
NotI NruI PacI PasI PmlI PsiI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI