Restriction map for pF1KB8425
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflIII      AgeI        ApaI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AvrII       BalI        BamHI       BbeI        BbrPI       
BcgI        BciVI       BclI        BglI        BlnI        
BlpI        Bpu1102I    BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BspDI       BspEI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraIII      
DrdI        DsaI        EagI        EclXI       Eco52I      
EcoNI       EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        Hin1I       HpaI        KasI        KpnI        
KspI        MfeI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       SphI        Sse8387I    SspI        SwaI        
XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AflII        1777  CTTAAG               C^TTAAG
AhdI          174  GACACTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1515  GTGCAC               G^TGCAC
AocI         1441  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       702  AGCGCT               AGC^GCT
ApaLI        1515  GTGCAC               G^TGCAC
AspEI         174  GACACTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          826  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         960  TGCGCA               TGC^GCA
BanI          870  GGTGCC               G^G[CT][GA]CC
BanII        1558  GAGCCC               G[GA]GC[CT]^C
BbvCI        1622  CCTCAGC              CCTCAGC(-5/-2)
BfrI         1777  CTTAAG               C^TTAAG
BglII        1256  AGATCT               A^GATCT
BsaAI        1090  TACGTG               [CT]AC^GT[GA]
BsaBI         200  GATTTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          708  CTGCAC               GTGCAG(16/14)
BsmI          230  GCATTC               GAATGC(1/-1)
Bsp1407I     1713  TGTACA               T^GTACA
BsrGI        1713  TGTACA               T^GTACA
Bsu36I       1441  CCTGAGG              CC^T[ACGT]AGG
DraII        1771  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EaeI          697  CGGCCA               [CT]^GGCC[GA]
Eam1105I      174  GACACTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      702  AGCGCT               AGC^GCT
Eco81I       1441  CCTGAGG              CC^T[ACGT]AGG
EcoO109I     1771  AGGCCCC              [GA]G^G[ACGT]CC[CT]
FauI           82  CCCGC                CCCGC(4/6)
FspI          960  TGCGCA               TGC^GCA
HaeII         702  AGCGCT               [GA]GCGC^[CT]
HapII         564  CCGG                 C^CGG
HgaI         1729  GACGC                GACGC(5/10)
HincII       1465  GTCAAC               GT[CT]^[GA]AC
HindII       1465  GTCAAC               GT[CT]^[GA]AC
HindIII      1689  AAGCTT               A^AGCTT
HpaII         564  CCGG                 C^CGG
MaeII        1091  ACGT                 A^CGT
MamI          200  GATTTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI          564  CCGG                 C^CGG
PflFI         826  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1588  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI         1048  GAAGAGC              GCTCTTC(1/4)
SmaI          563  CCCGGG               CCC^GGG
SmlI         1777  CTTAAG               C^T[CT][GA]AG
SpeI         1162  ACTAGT               A^CTAGT
SspBI        1713  TGTACA               T^GTACA
StuI         1085  AGGCCT               AGG^CCT
Tth111I       826  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1588  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1305  TCTAGA               T^CTAGA
XmaCI         563  CCCGGG               C^CCGGG
XmaI          563  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AlwNI         256  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              908  CAGTGGCTG           
AspHI        1096  GAGCAC               G[AT]GC[AT]^C
             1515  GTGCAC              
AvaI          563  CCCGGG               C^[CT]CG[GA]G
             1849  CCCGAG              
AvaII        1222  GGACC                G^G[AT]CC
             1935  GGTCC               
BcnI          563  CCCGG                CC^[GC]GG
              564  CCGGG               
BsaI          491  GAGACC               GGTCTC(1/5)
             1209  GGTCTC              
BsiHKAI      1096  GAGCAC               G[AT]GC[AT]^C
             1515  GTGCAC              
BsmFI        1509  GTCCC                GGGAC(10/14)
             1936  GTCCC               
BsoBI         563  CCCGGG               C^[CT]CG[GA]G
             1849  CCCGAG              
BstNI         261  CCTGG                CC^[AT]GG
             1588  CCAGG               
CfoI          703  GCGC                 GCG^C
              961  GCGC                
DraI           21  TTTAAA               TTT^AAA
              811  TTTAAA              
EcoRII        261  CCTGG                ^CC[AT]GG
             1588  CCAGG               
EcoT14I      1523  CCTTGG               C^C[AT][AT]GG
             1678  CCTTGG              
HhaI          703  GCGC                 GCG^C
              961  GCGC                
HinP1I        703  GCGC                 G^CGC
              961  GCGC                
MslI          364  CACAATAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              553  CATGGACATG          
MvaI          261  CCTGG                CC^[AT]GG
             1588  CCAGG               
NciI          563  CCCGG                CC^[GC]GG
              564  CCGGG               
NspI          558  ACATGC               [GA]CATG^[CT]
             1053  GCATGT              
PstI          290  CTGCAG               CTGCA^G
             1988  CTGCAG              
PvuII         834  CAGCTG               CAG^CTG
             1114  CAGCTG              
SfcI          290  CTGCAG               C^T[GA][CT]AG
             1988  CTGCAG              
StyI         1523  CCTTGG               C^C[AT][AT]GG
             1678  CCTTGG              
TaqI         1082  TCGA                 T^CGA
             1742  TCGA                
TthHB8I      1082  TCGA                 T^CGA
             1742  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BbsI        BbvI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BseRI       BsiYI       
BslI        BsmAI       Bsp1286I    BspHI       BsrI        
BssKI       BstF5I      BstYI       Cac8I       Cfr13I      
DdeI        DpnII       EarI        EcoRI       Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspA1I      MwoI        
NdeII       NlaIII      NlaIV       PleI        RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XhoII       
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