Restriction map for pF1KB8425
 (Linear) (Six-base) MAPSORT of: KIBB8425.seq  Check: 6892  from: 1  to: 2064



KIBB8425.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     533     717    1138    1685    2036    2064 
   Size:         95     438     184     421     547     351      28

  Fragments arranged by size: 

                 547     438     421     351     184      95      28
 
AfeI AGC'GCT

Cuts at:      0     704    2064 
   Size:        704    1360
 
AflII C'TTAA_G

Cuts at:      0    1777    2064 
   Size:       1777     287
 
AhdI GACnn_n'nnGTC

Cuts at:      0     179    2064 
   Size:        179    1885
 
AlwNI CAG_nnn'CTG

Cuts at:      0     261     913    2064 
   Size:        261     652    1151

  Fragments arranged by size: 

                1151     652     261
 
ApaLI G'TGCA_C

Cuts at:      0    1515    2064 
   Size:       1515     549
 
ApoI r'AATT_y

Cuts at:      0     103     529     759     805     814    1336    1810    1981 
   Size:        103     426     230      46       9     522     474     171

Cuts at:   1981    2064 
   Size:         83

  Fragments arranged by size: 

                 522     474     426     230     171     103      83      46
                   9
 
AvaI C'yCGr_G

Cuts at:      0     563    1849    2064 
   Size:        563    1286     215

  Fragments arranged by size: 

                1286     563     215
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1565    1598    2064 
   Size:       1565      33     466

  Fragments arranged by size: 

                1565     466      33
 
BanI G'GyrC_C

Cuts at:      0     870    2064 
   Size:        870    1194
 
BanII G_rGCy'C

Cuts at:      0    1562    2064 
   Size:       1562     502
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     146     178     278    2064 
   Size:        146      32     100    1786

  Fragments arranged by size: 

                1786     146     100      32
 
BbvCI CC'TCA_GC

Cuts at:      0    1623    2064 
   Size:       1623     441
 
BglII A'GATC_T

Cuts at:      0    1256    2064 
   Size:       1256     808
 
Bme1580I G_kGCm'C

Cuts at:      0    1519    2064 
   Size:       1519     545
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1506    1954    2055    2064 
   Size:       1506     448     101       9

  Fragments arranged by size: 

                1506     448     101       9
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     509     627    1012    1452    2064 
   Size:        509     118     385     440     612

  Fragments arranged by size: 

                 612     509     440     385     118
 
Bpu10I CC'TnA_GC

Cuts at:      0    1623    2064 
   Size:       1623     441
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     485    1215    2064 
   Size:        485     730     849

  Fragments arranged by size: 

                 849     730     485
 
BsaAI yAC'GTr

Cuts at:      0    1092    2064 
   Size:       1092     972
 
BsaBI GATnn'nnATC

Cuts at:      0     204    2064 
   Size:        204    1860
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     486     808    1256    1432    1459    2064 
   Size:        486     322     448     176      27     605

  Fragments arranged by size: 

                 605     486     448     322     176      27
 
BseYI C'CCAG_C

Cuts at:      0     304     495    1146    1561    2021    2064 
   Size:        304     191     651     415     460      43

  Fragments arranged by size: 

                 651     460     415     304     191      43
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     693    2064 
   Size:        693    1371
 
BsiHKAI G_wGCw'C

Cuts at:      0    1100    1519    2064 
   Size:       1100     419     545

  Fragments arranged by size: 

                1100     545     419
 
BsmI GAATG_Cn'

Cuts at:      0     230    2064 
   Size:        230    1834
 
Bsp1286I G_dGCh'C

Cuts at:      0    1100    1519    1562    2064 
   Size:       1100     419      43     502

  Fragments arranged by size: 

                1100     502     419      43
 
BspHI T'CATG_A

Cuts at:      0     342     723    1044    2064 
   Size:        342     381     321    1020

  Fragments arranged by size: 

                1020     381     342     321
 
BsrGI T'GTAC_A

Cuts at:      0    1713    2064 
   Size:       1713     351
 
BstYI r'GATC_y

Cuts at:      0     308    1256    1845    2064 
   Size:        308     948     589     219

  Fragments arranged by size: 

                 948     589     308     219
 
Bsu36I CC'TnA_GG

Cuts at:      0    1442    2064 
   Size:       1442     622
 
BtsI GCAGTG_nn'

Cuts at:      0    1208    1268    1601    2064 
   Size:       1208      60     333     463

  Fragments arranged by size: 

                1208     463     333      60
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1124    1159    2064 
   Size:       1124      35     905

  Fragments arranged by size: 

                1124     905      35
 
DraI TTT'AAA

Cuts at:      0      23     813    2064 
   Size:         23     790    1251

  Fragments arranged by size: 

                1251     790      23
 
EaeI y'GGCC_r

Cuts at:      0     697    2064 
   Size:        697    1367
 
EarI CTCTTCn'nnn_

Cuts at:      0     509     778     785     881    1043    1529    1661    2064 
   Size:        509     269       7      96     162     486     132     403

  Fragments arranged by size: 

                 509     486     403     269     162     132      96       7
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     509     533     627     717    1012    1138    1452 
   Size:         95     414      24      94      90     295     126     314

Cuts at:   1452    1685    2036    2064 
   Size:        233     351      28

  Fragments arranged by size: 

                 414     351     314     295     233     126      95      94
                  90      28      24
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1772    2064 
   Size:       1772     292
 
EcoRI G'AATT_C

Cuts at:      0     529     805    1810    2064 
   Size:        529     276    1005     254

  Fragments arranged by size: 

                1005     529     276     254
 
FspI TGC'GCA

Cuts at:      0     962    2064 
   Size:        962    1102
 
HaeII r_GCGC'y

Cuts at:      0     706    2064 
   Size:        706    1358
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     165     197     484     516     625     657    1492    1524 
   Size:        165      32     287      32     109      32     835      32

Cuts at:   1524    1583    1615    1939    1971    2064 
   Size:         59      32     324      32      93

  Fragments arranged by size: 

                 835     324     287     165     109      93      59      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1467    2064 
   Size:       1467     597
 
HindIII A'AGCT_T

Cuts at:      0    1689    2064 
   Size:       1689     375
 
MslI CAynn'nnrTG

Cuts at:      0     368     557    2064 
   Size:        368     189    1507

  Fragments arranged by size: 

                1507     368     189
 
MspA1I CmG'CkG

Cuts at:      0      85     631     836    1116    1627    2064 
   Size:         85     546     205     280     511     437

  Fragments arranged by size: 

                 546     511     437     280     205      85
 
NspI r_CATG'y

Cuts at:      0     562    1057    2064 
   Size:        562     495    1007

  Fragments arranged by size: 

                1007     562     495
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1594    2064 
   Size:       1594     470
 
PstI C_TGCA'G

Cuts at:      0     294    1992    2064 
   Size:        294    1698      72

  Fragments arranged by size: 

                1698     294      72
 
PvuII CAG'CTG

Cuts at:      0     836    1116    2064 
   Size:        836     280     948

  Fragments arranged by size: 

                 948     836     280
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1043    2064 
   Size:       1043    1021
 
SfcI C'TryA_G

Cuts at:      0     290    1988    2064 
   Size:        290    1698      76

  Fragments arranged by size: 

                1698     290      76
 
SmaI CCC'GGG

Cuts at:      0     565    2064 
   Size:        565    1499
 
SmlI C'TyrA_G

Cuts at:      0    1777    2064 
   Size:       1777     287
 
SpeI A'CTAG_T

Cuts at:      0    1162    2064 
   Size:       1162     902
 
StuI AGG'CCT

Cuts at:      0    1087    2064 
   Size:       1087     977
 
StyI C'CwwG_G

Cuts at:      0    1523    1678    2064 
   Size:       1523     155     386

  Fragments arranged by size: 

                1523     386     155
 
TatI w'GTAC_w

Cuts at:      0      87     211     252    1713    2064 
   Size:         87     124      41    1461     351

  Fragments arranged by size: 

                1461     351     124      87      41
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     575     607     998    1030    1214    1246    2064 
   Size:        575      32     391      32     184      32     818

  Fragments arranged by size: 

                 818     575     391     184      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     829    2064 
   Size:        829    1235
 
XbaI T'CTAG_A

Cuts at:      0    1305    2064 
   Size:       1305     759
 
XmaI C'CCGG_G

Cuts at:      0     563    2064 
   Size:        563    1501
 

 Enzymes that do cut:

     AcuI     AfeI    AflII     AhdI    AlwNI    ApaLI     ApoI     AvaI
     BaeI     BanI    BanII     BbsI    BbvCI    BglII Bme1580I     BmrI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BsrGI    BstYI   Bsu36I     BtsI
    CspCI     DraI     EaeI     EarI  Eco57MI EcoO109I    EcoRI     FspI
    HaeII    Hin4I   HincII  HindIII     MslI   MspA1I     NspI    PflMI
     PstI    PvuII     SapI     SfcI     SmaI     SmlI     SpeI     StuI
     StyI     TatI     TstI  Tth111I     XbaI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI   AflIII     AgeI     AleI
     AlfI     AloI     ApaI     AscI     AseI    AsiSI    AvrII    BamHI
     BbeI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI
     BmtI     BplI    BpuEI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI
    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FalI
     FseI    FspAI     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SphI     SrfI     SspI
     SwaI    TaqII     XcmI     XhoI     XmnI     ZraI
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