Restriction map for pF1KB8416
##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AspHI       AvaI        AviII       AvrII       BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BglI        BlnI        BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsoBI       BspEI       
BspLU11I    BsrBI       BsrFI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     Cfr10I      CpoI        DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     FauI        FseI        FspI        
HaeII       KasI        KpnI        KspI        MluI        
MroI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        RsrII       SacI        SacII       SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SpeI        SphI        Sse8387I    
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AcyI           42  GGCGTC               G[GA]^CG[CT]C
AocI          955  CCTGAGG              CC^T[ACGT]AGG
AseI          748  ATTAAT               AT^TAAT
AsnI          748  ATTAAT               AT^TAAT
AspI         1821  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          176  TGGCCA               TGG^CCA
BanI          960  GGCACC               G^G[CT][GA]CC
BbsI         2072  GAAGAC               GAAGAC(2/6)
BclI         2559  TGATCA               T^GATCA
BcnI         3115  CCGGG                CC^[GC]GG
BglII         438  AGATCT               A^GATCT
BlpI         1742  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     1742  GCTTAGC              GC^T[ACGT]AGC
BpuAI        2072  GAAGAC               GAAGAC(2/6)
BsaAI        1726  TACGTA               [CT]AC^GT[GA]
BsaBI        2554  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          42  GGCGTC               G[GA]^CG[CT]C
BseRI        1316  GAGGAG               GAGGAG(10/8)
BsgI          554  GTGCAG               GTGCAG(16/14)
BspMI        2673  ACCTGC               ACCTGC(4/8)
BssSI        1313  CACGAG               CACGAG(-5/-1)
BstXI         827  CCACAGAACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        955  CCTGAGG              CC^T[ACGT]AGG
CelII        1742  GCTTAGC              GC^T[ACGT]AGC
CfoI         2652  GCGC                 GCG^C
DraII        3117  GGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII       1356  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I        955  CCTGAGG              CC^T[ACGT]AGG
EcoO109I     3117  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI        2380  GAATTC               G^AATTC
EcoRV         795  GATATC               GAT^ATC
FbaI         2559  TGATCA               T^GATCA
HapII        3115  CCGG                 C^CGG
HgaI           43  GCGTC                GACGC(5/10)
HhaI         2652  GCGC                 GCG^C
Hin1I          42  GGCGTC               G[GA]^CG[CT]C
HinP1I       2652  GCGC                 G^CGC
HindIII      2756  AAGCTT               A^AGCTT
HpaI         3018  GTTAAC               GTT^AAC
HpaII        3115  CCGG                 C^CGG
MamI         2554  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2792  CAATTG               C^AATTG
MluNI         176  TGGCCA               TGG^CCA
MscI          176  TGGCCA               TGG^CCA
MspI         3115  CCGG                 C^CGG
MunI         2792  CAATTG               C^AATTG
NciI         3115  CCGGG                CC^[GC]GG
PflFI        1821  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        3117  GGGACCT              [GA]G^G[AT]CC[CT]
PshBI         748  ATTAAT               AT^TAAT
SalI          134  GTCGAC               G^TCGAC
SapI         2143  GCTCTTC              GCTCTTC(1/4)
SnaBI        1726  TACGTA               TAC^GTA
StuI         1756  AGGCCT               AGG^CCT
SwaI         2243  ATTTAAAT             ATTT^AAAT
Tth111I      1821  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI          134  GTCGAC               GT^[AC][GT]AC
             1729  GTAGAC              
AciI           23  CCGC                 CCGC(-3/-1)
             1813  CCGC                
AflII         195  CTTAAG               C^TTAAG
             1335  CTTAAG              
Asp700I      1223  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1300  GAAGAATTTC          
BamHI           7  GGATCC               G^GATCC
             1845  GGATCC              
BfrI          195  CTTAAG               C^TTAAG
             1335  CTTAAG              
BmyI          531  GTGCCC               G[AGT]GC[ACT]^C
             1782  GGGCAC              
BsaI          430  GAGACC               GGTCTC(1/5)
             1648  GAGACC              
BsmI         2277  GAATGC               GAATGC(1/-1)
             2466  GAATGC              
Bsp1286I      531  GTGCCC               G[AGT]GC[ACT]^C
             1782  GGGCAC              
Bsp1407I      561  TGTACA               T^GTACA
             2615  TGTACA              
BspDI         115  ATCGAT               AT^CGAT
             2867  ATCGAT              
BspHI         270  TCATGA               T^CATGA
             3144  TCATGA              
BsrDI         494  CATTGC               GCAATG(2/0)
              163  GCAATG              
BsrGI         561  TGTACA               T^GTACA
             2615  TGTACA              
ClaI          115  ATCGAT               AT^CGAT
             2867  ATCGAT              
EaeI           20  TGGCCG               [CT]^GGCC[GA]
              176  TGGCCA              
EcoT22I       122  ATGCAT               ATGCA^T
             3025  ATGCAT              
MslI          159  CACAGCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              978  CACAGCTATG          
NsiI          122  ATGCAT               ATGCA^T
             3025  ATGCAT              
PflMI        1027  CCAAATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1910  CCATACAGTGG         
PstI         1172  CTGCAG               CTGCA^G
             1994  CTGCAG              
RcaI          270  TCATGA               T^CATGA
             3144  TCATGA              
SmlI          195  CTTAAG               C^T[CT][GA]AG
             1335  CTTAAG              
SspBI         561  TGTACA               T^GTACA
             2615  TGTACA              
SspI         2126  AATATT               AAT^ATT
             2353  AATATT              
Van91I       1027  CCAAATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1910  CCATACAGTGG         
XmnI         1223  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1300  GAAGAATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        AlwNI       
ApoI        AvaII       BbvI        BfaI        BpmI        
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraI        
EarI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HincII      HindII      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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