Restriction map for pF1KB8416
 (Linear) (Six-base) MAPSORT of: KIBB8416.seq  Check: 9722  from: 1  to: 3156



KIBB8416.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AccI GT'mk_AC

Cuts at:      0     135    1730    3156 
   Size:        135    1595    1426

  Fragments arranged by size: 

                1595    1426     135
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     377    1820    2131    2257    2509    2837    3123    3156 
   Size:        377    1443     311     126     252     328     286      33

  Fragments arranged by size: 

                1443     377     328     311     286     252     126      33
            
 
AflII C'TTAA_G

Cuts at:      0     195    1335    3156 
   Size:        195    1140    1821

  Fragments arranged by size: 

                1821    1140     195
 
AlwNI CAG_nnn'CTG

Cuts at:      0     106    1371    1492    2704    3156 
   Size:        106    1265     121    1212     452

  Fragments arranged by size: 

                1265    1212     452     121     106
 
ApoI r'AATT_y

Cuts at:      0      81     569     604     644    1225    1303    1377    1431 
   Size:         81     488      35      40     581      78      74      54

Cuts at:   1431    1596    1972    2236    2380    3156 
   Size:        165     376     264     144     776

  Fragments arranged by size: 

                 776     581     488     376     264     165     144      81
                  78      74      54      40      35
 
AseI AT'TA_AT

Cuts at:      0     749    3156 
   Size:        749    2407
 
BamHI G'GATC_C

Cuts at:      0       7    1845    3156 
   Size:          7    1838    1311

  Fragments arranged by size: 

                1838    1311       7
 
BanI G'GyrC_C

Cuts at:      0     960    3156 
   Size:        960    2196
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2079    3156 
   Size:       2079    1077
 
BclI T'GATC_A

Cuts at:      0    2559    3156 
   Size:       2559     597
 
BfrBI ATG'CAT

Cuts at:      0     124    3027    3156 
   Size:        124    2903     129

  Fragments arranged by size: 

                2903     129     124
 
BglII A'GATC_T

Cuts at:      0     438    3156 
   Size:        438    2718
 
BlpI GC'TnA_GC

Cuts at:      0    1743    3156 
   Size:       1743    1413
 
Bme1580I G_kGCm'C

Cuts at:      0     535    1786    3156 
   Size:        535    1251    1370

  Fragments arranged by size: 

                1370    1251     535
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2085    3156 
   Size:       2085    1071
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     736     925    3071    3107    3156 
   Size:        736     189    2146      36      49

  Fragments arranged by size: 

                2146     736     189      49      36
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     424    1642    3156 
   Size:        424    1218    1514

  Fragments arranged by size: 

                1514    1218     424
 
BsaAI yAC'GTr

Cuts at:      0    1728    3156 
   Size:       1728    1428
 
BsaBI GATnn'nnATC

Cuts at:      0    2558    3156 
   Size:       2558     598
 
BsaHI Gr'CG_yC

Cuts at:      0      43    3156 
   Size:         43    3113
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     898     928    3156 
   Size:        898      30    2228

  Fragments arranged by size: 

                2228     898      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1331    3156 
   Size:       1331    1825
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     575    3156 
   Size:        575    2581
 
BsmI GAATG_Cn'

Cuts at:      0    2283    2472    3156 
   Size:       2283     189     684

  Fragments arranged by size: 

                2283     684     189
 
Bsp1286I G_dGCh'C

Cuts at:      0     535    1786    3156 
   Size:        535    1251    1370

  Fragments arranged by size: 

                1370    1251     535
 
BspHI T'CATG_A

Cuts at:      0     270    3144    3156 
   Size:        270    2874      12

  Fragments arranged by size: 

                2874     270      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2682    3156 
   Size:       2682     474
 
BsrDI GCAATG_nn'

Cuts at:      0     170     493    3156 
   Size:        170     323    2663

  Fragments arranged by size: 

                2663     323     170
 
BsrGI T'GTAC_A

Cuts at:      0     561    2615    3156 
   Size:        561    2054     541

  Fragments arranged by size: 

                2054     561     541
 
BssSI C'ACGA_G

Cuts at:      0    1313    3156 
   Size:       1313    1843
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     834    3156 
   Size:        834    2322
 
BstYI r'GATC_y

Cuts at:      0       7     297     438     772    1488    1845    3066    3156 
   Size:          7     290     141     334     716     357    1221      90

  Fragments arranged by size: 

                1221     716     357     334     290     141      90       7
            
 
Bsu36I CC'TnA_GG

Cuts at:      0     956    3156 
   Size:        956    2200
 
BtsI GCAGTG_nn'

Cuts at:      0    1568    2457    2540    2690    3156 
   Size:       1568     889      83     150     466

  Fragments arranged by size: 

                1568     889     466     150      83
 
ClaI AT'CG_AT

Cuts at:      0     116    2868    3156 
   Size:        116    2752     288

  Fragments arranged by size: 

                2752     288     116
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2936    2971    3156 
   Size:       2936      35     185

  Fragments arranged by size: 

                2936     185      35
 
DraI TTT'AAA

Cuts at:      0     767    1382    2246    3156 
   Size:        767     615     864     910

  Fragments arranged by size: 

                 910     864     767     615
 
DraIII CAC_nnn'GTG

Cuts at:      0    1361    3156 
   Size:       1361    1795
 
EaeI y'GGCC_r

Cuts at:      0      20     176    3156 
   Size:         20     156    2980

  Fragments arranged by size: 

                2980     156      20
 
EarI CTCTTCn'nnn_

Cuts at:      0      32    1091    1850    2150    2318    3156 
   Size:         32    1059     759     300     168     838

  Fragments arranged by size: 

                1059     838     759     300     168      32
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     377     736     925    1820    2131    2257    2509    2837 
   Size:        377     359     189     895     311     126     252     328

Cuts at:   2837    3071    3107    3123    3156 
   Size:        234      36      16      33

  Fragments arranged by size: 

                 895     377     359     328     311     252     234     189
                 126      36      33      16
 
EcoO109I rG'GnC_Cy

Cuts at:      0    3118    3156 
   Size:       3118      38
 
EcoRI G'AATT_C

Cuts at:      0    2380    3156 
   Size:       2380     776
 
EcoRV GAT'ATC

Cuts at:      0     797    3156 
   Size:        797    2359
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     382    3156 
   Size:        382    2774
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     195     227     423     455    2317    2349    3156 
   Size:        195      32     196      32    1862      32     807

  Fragments arranged by size: 

                1862     807     196     195      32      32      32
 
HincII GTy'rAC

Cuts at:      0      98     136    3020    3156 
   Size:         98      38    2884     136

  Fragments arranged by size: 

                2884     136      98      38
 
HindIII A'AGCT_T

Cuts at:      0    2756    3156 
   Size:       2756     400
 
HpaI GTT'AAC

Cuts at:      0    3020    3156 
   Size:       3020     136
 
MfeI C'AATT_G

Cuts at:      0    2792    3156 
   Size:       2792     364
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1014    1535    1663    2502    3156 
   Size:       1014     521     128     839     654

  Fragments arranged by size: 

                1014     839     654     521     128
 
MscI TGG'CCA

Cuts at:      0     178    3156 
   Size:        178    2978
 
MslI CAynn'nnrTG

Cuts at:      0     163     982    3156 
   Size:        163     819    2174

  Fragments arranged by size: 

                2174     819     163
 
MspA1I CmG'CkG

Cuts at:      0    1894    2590    2632    3156 
   Size:       1894     696      42     524

  Fragments arranged by size: 

                1894     696     524      42
 
NsiI A_TGCA'T

Cuts at:      0     126    3029    3156 
   Size:        126    2903     127

  Fragments arranged by size: 

                2903     127     126
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1033    1916    3156 
   Size:       1033     883    1240

  Fragments arranged by size: 

                1240    1033     883
 
PpuMI rG'GwC_Cy

Cuts at:      0    3118    3156 
   Size:       3118      38
 
PsiI TTA'TAA

Cuts at:      0    2056    3156 
   Size:       2056    1100
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     830     862    1895    1927    3156 
   Size:        830      32    1033      32    1229

  Fragments arranged by size: 

                1229    1033     830      32      32
 
PstI C_TGCA'G

Cuts at:      0    1176    1998    3156 
   Size:       1176     822    1158

  Fragments arranged by size: 

                1176    1158     822
 
PvuII CAG'CTG

Cuts at:      0    1894    2590    2632    3156 
   Size:       1894     696      42     524

  Fragments arranged by size: 

                1894     696     524      42
 
SalI G'TCGA_C

Cuts at:      0     134    3156 
   Size:        134    3022
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2150    3156 
   Size:       2150    1006
 
SfcI C'TryA_G

Cuts at:      0    1172    1994    2297    2678    2696    3156 
   Size:       1172     822     303     381      18     460

  Fragments arranged by size: 

                1172     822     460     381     303      18
 
SmlI C'TyrA_G

Cuts at:      0     195    1335    3156 
   Size:        195    1140    1821

  Fragments arranged by size: 

                1821    1140     195
 
SnaBI TAC'GTA

Cuts at:      0    1728    3156 
   Size:       1728    1428
 
SspI AAT'ATT

Cuts at:      0    2128    2355    3156 
   Size:       2128     227     801

  Fragments arranged by size: 

                2128     801     227
 
StuI AGG'CCT

Cuts at:      0    1758    3156 
   Size:       1758    1398
 
StyI C'CwwG_G

Cuts at:      0     992    1091    1149    2717    3156 
   Size:        992      99      58    1568     439

  Fragments arranged by size: 

                1568     992     439      99      58
 
SwaI ATTT'AAAT

Cuts at:      0    2246    3156 
   Size:       2246     910
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     196    3156 
   Size:        196    2960
 
TatI w'GTAC_w

Cuts at:      0     363     561    1873    2615    3156 
   Size:        363     198    1312     742     541

  Fragments arranged by size: 

                1312     742     541     363     198
 
Tth111I GACn'n_nGTC

Cuts at:      0    1824    3156 
   Size:       1824    1332
 
XmnI GAAnn'nnTTC

Cuts at:      0    1227    1304    3156 
   Size:       1227      77    1852

  Fragments arranged by size: 

                1852    1227      77
 

 Enzymes that do cut:

     AccI     AcuI    AflII    AlwNI     ApoI     AseI    BamHI     BanI
     BbsI     BclI    BfrBI    BglII     BlpI Bme1580I     BmrI     BpmI
     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI     BsgI     BsmI
 Bsp1286I    BspHI    BspMI    BsrDI    BsrGI    BssSI    BstXI    BstYI
   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     EaeI     EarI
  Eco57MI EcoO109I    EcoRI    EcoRV     FalI    Hin4I   HincII  HindIII
     HpaI     MfeI     MmeI     MscI     MslI   MspA1I     NsiI    PflMI
    PpuMI     PsiI     PsrI     PstI    PvuII     SalI     SapI     SfcI
     SmlI    SnaBI     SspI     StuI     StyI     SwaI    TaqII     TatI
  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BanII     BbeI    BbvCI     BcgI    BciVI     BglI
    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaWI    BseYI    BsiEI
  BsiHKAI    BsiWI    BsmBI    BspEI    BsrBI    BsrFI   BssHII   BstAPI
    BstBI   BstEII  BstZ17I     BtgI    BtgZI     DrdI     EagI     EciI
  EcoICRI    EcoNI     FseI     FspI    FspAI    HaeII     KasI     KpnI
     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PshAI   PspOMI    PspXI     PvuI    RsrII     SacI    SacII
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SpeI     SphI     SrfI     TstI     XbaI     XcmI     XhoI
     XmaI     ZraI
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