Restriction map for pF1KB8408
 (Linear) (Six-base) MAPSORT of: KIBB8408.seq  Check: 5804  from: 1  to: 1710



KIBB8408.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     246     590    1177    1361    1710 
   Size:        148      98     344     587     184     349

  Fragments arranged by size: 

                 587     349     344     184     148      98
 
AflII C'TTAA_G

Cuts at:      0    1536    1710 
   Size:       1536     174
 
AflIII A'CryG_T

Cuts at:      0     158    1165    1546    1710 
   Size:        158    1007     381     164

  Fragments arranged by size: 

                1007     381     164     158
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     539    1125    1710 
   Size:        539     586     585

  Fragments arranged by size: 

                 586     585     539
 
ApaLI G'TGCA_C

Cuts at:      0    1050    1710 
   Size:       1050     660
 
ApoI r'AATT_y

Cuts at:      0    1060    1400    1710 
   Size:       1060     340     310

  Fragments arranged by size: 

                1060     340     310
 
AseI AT'TA_AT

Cuts at:      0     679    1333    1710 
   Size:        679     654     377

  Fragments arranged by size: 

                 679     654     377
 
AvrII C'CTAG_G

Cuts at:      0     834    1005    1710 
   Size:        834     171     705

  Fragments arranged by size: 

                 834     705     171
 
BamHI G'GATC_C

Cuts at:      0    1000    1710 
   Size:       1000     710
 
BanII G_rGCy'C

Cuts at:      0    1597    1710 
   Size:       1597     113
 
BbvCI CC'TCA_GC

Cuts at:      0     180    1710 
   Size:        180    1530
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     644    1710 
   Size:        644    1066
 
BfrBI ATG'CAT

Cuts at:      0     921    1710 
   Size:        921     789
 
BglII A'GATC_T

Cuts at:      0     864    1710 
   Size:        864     846
 
BlpI GC'TnA_GC

Cuts at:      0    1596    1710 
   Size:       1596     114
 
Bme1580I G_kGCm'C

Cuts at:      0    1054    1359    1710 
   Size:       1054     305     351

  Fragments arranged by size: 

                1054     351     305
 
BmgBI CAC'GTC

Cuts at:      0    1549    1710 
   Size:       1549     161
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     415    1710 
   Size:        415    1295
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     785    1710 
   Size:        785     925
 
Bpu10I CC'TnA_GC

Cuts at:      0     180    1710 
   Size:        180    1530
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     376     700    1186    1710 
   Size:        376     324     486     524

  Fragments arranged by size: 

                 524     486     376     324
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     394    1710 
   Size:        394    1316
 
BsaAI yAC'GTr

Cuts at:      0     159     861    1166    1710 
   Size:        159     702     305     544

  Fragments arranged by size: 

                 702     544     305     159
 
BsaWI w'CCGG_w

Cuts at:      0     745    1710 
   Size:        745     965
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     143     173    1710 
   Size:        143      30    1537

  Fragments arranged by size: 

                1537     143      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121    1710 
   Size:        121    1589
 
BsiHKAI G_wGCw'C

Cuts at:      0    1054    1710 
   Size:       1054     656
 
BsmI GAATG_Cn'

Cuts at:      0    1292    1710 
   Size:       1292     418
 
Bsp1286I G_dGCh'C

Cuts at:      0    1054    1359    1597    1710 
   Size:       1054     305     238     113

  Fragments arranged by size: 

                1054     305     238     113
 
BspEI T'CCGG_A

Cuts at:      0     745    1710 
   Size:        745     965
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     492    1710 
   Size:        492    1218
 
BsrGI T'GTAC_A

Cuts at:      0     163     733    1710 
   Size:        163     570     977

  Fragments arranged by size: 

                 977     570     163
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1188    1710 
   Size:       1188     522
 
BstYI r'GATC_y

Cuts at:      0     224     443     864    1000    1680    1710 
   Size:        224     219     421     136     680      30

  Fragments arranged by size: 

                 680     421     224     219     136      30
 
Bsu36I CC'TnA_GG

Cuts at:      0     117    1710 
   Size:        117    1593
 
BtgI C'CryG_G

Cuts at:      0    1210    1710 
   Size:       1210     500
 
BtsI GCAGTG_nn'

Cuts at:      0    1052    1139    1177    1359    1710 
   Size:       1052      87      38     182     351

  Fragments arranged by size: 

                1052     351     182      87      38
 
DraI TTT'AAA

Cuts at:      0     221     611    1710 
   Size:        221     390    1099

  Fragments arranged by size: 

                1099     390     221
 
DraIII CAC_nnn'GTG

Cuts at:      0    1489    1710 
   Size:       1489     221
 
EaeI y'GGCC_r

Cuts at:      0    1468    1710 
   Size:       1468     242
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     246     590     785    1177    1361    1710 
   Size:        148      98     344     195     392     184     349

  Fragments arranged by size: 

                 392     349     344     195     184     148      98
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     120     611    1710 
   Size:        120     491    1099

  Fragments arranged by size: 

                1099     491     120
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1665    1697    1710 
   Size:       1665      32      13

  Fragments arranged by size: 

                1665      32      13
 
HincII GTy'rAC

Cuts at:      0    1196    1710 
   Size:       1196     514
 
HindIII A'AGCT_T

Cuts at:      0     258    1633    1710 
   Size:        258    1375      77

  Fragments arranged by size: 

                1375     258      77
 
HpaI GTT'AAC

Cuts at:      0    1196    1710 
   Size:       1196     514
 
MscI TGG'CCA

Cuts at:      0    1470    1710 
   Size:       1470     240
 
MslI CAynn'nnrTG

Cuts at:      0      30     521     661    1411    1710 
   Size:         30     491     140     750     299

  Fragments arranged by size: 

                 750     491     299     140      30
 
MspA1I CmG'CkG

Cuts at:      0     135     825    1710 
   Size:        135     690     885

  Fragments arranged by size: 

                 885     690     135
 
NcoI C'CATG_G

Cuts at:      0    1210    1710 
   Size:       1210     500
 
NdeI CA'TA_TG

Cuts at:      0    1689    1710 
   Size:       1689      21
 
NsiI A_TGCA'T

Cuts at:      0     923    1710 
   Size:        923     787
 
NspI r_CATG'y

Cuts at:      0     520    1186    1710 
   Size:        520     666     524

  Fragments arranged by size: 

                 666     524     520
 
PmlI CAC'GTG

Cuts at:      0    1166    1710 
   Size:       1166     544
 
PstI C_TGCA'G

Cuts at:      0    1114    1193    1710 
   Size:       1114      79     517

  Fragments arranged by size: 

                1114     517      79
 
PvuII CAG'CTG

Cuts at:      0     135     825    1710 
   Size:        135     690     885

  Fragments arranged by size: 

                 885     690     135
 
ScaI AGT'ACT

Cuts at:      0     438    1710 
   Size:        438    1272
 
SfcI C'TryA_G

Cuts at:      0    1110    1189    1645    1655    1710 
   Size:       1110      79     456      10      55

  Fragments arranged by size: 

                1110     456      79      55      10
 
SmlI C'TyrA_G

Cuts at:      0     391     715    1201    1536    1710 
   Size:        391     324     486     335     174

  Fragments arranged by size: 

                 486     391     335     324     174
 
StyI C'CwwG_G

Cuts at:      0     834    1005    1210    1613    1710 
   Size:        834     171     205     403      97

  Fragments arranged by size: 

                 834     403     205     171      97
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     351    1710 
   Size:        351    1359
 
TatI w'GTAC_w

Cuts at:      0     163     436     733    1234    1312    1710 
   Size:        163     273     297     501      78     398

  Fragments arranged by size: 

                 501     398     297     273     163      78
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     143     175     764     796    1169    1201    1536    1568 
   Size:        143      32     589      32     373      32     335      32

Cuts at:   1568    1710 
   Size:        142

  Fragments arranged by size: 

                 589     373     335     143     142      32      32      32
                  32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1674    1710 
   Size:       1674      36
 
XmnI GAAnn'nnTTC

Cuts at:      0     314    1292    1710 
   Size:        314     978     418

  Fragments arranged by size: 

                 978     418     314
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AlfI    ApaLI     ApoI     AseI    AvrII
    BamHI    BanII    BbvCI    BciVI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI
    BsaXI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrGI
   BstAPI    BstYI   Bsu36I     BtgI     BtsI     DraI   DraIII     EaeI
  Eco57MI    EcoNI    Hin4I   HincII  HindIII     HpaI     MscI     MslI
   MspA1I     NcoI     NdeI     NsiI     NspI     PmlI     PstI    PvuII
     ScaI     SfcI     SmlI     StyI    TaqII     TatI     TstI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI     AscI    AsiSI     AvaI     BaeI
     BanI     BbeI     BbsI     BcgI     BclI     BglI     BmtI     BplI
    BsaBI    BsaHI    BseRI    BseYI    BsiEI    BsiWI    BsmBI    BspHI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII    BstXI
  BstZ17I    BtgZI     ClaI    CspCI     DrdI     EagI     EarI     EciI
  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI
     XhoI     XmaI     ZraI
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