Restriction map for pF1KB8400
##################################
# NO CUT ENZYMES (  179 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BcnI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsmFI       BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FseI        FspI        
HapII       HgaI        Hin1I       HincII      HindII      
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        Sse8387I    SspBI       StuI        SwaI        
TaqI        Tth111I     TthHB8I     Van91I      XbaI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AlwI          177  GATCC                GGATC(4/5)
AlwNI         134  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       160  GAAATCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII          96  GGACC                G^G[AT]CC
BanI          435  GGTGCC               G^G[CT][GA]CC
BanII         106  GAGCTC               G[GA]GC[CT]^C
BciVI         185  GTATCC               GTATCC(6/5)
BclI          453  TGATCA               T^GATCA
BglI          438  GCCACATGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaJI          59  CCATGG               C^C[ACGT][ACGT]GG
BsmAI         523  GAGAC                GTCTC(1/5)
BsmI          576  GCATTC               GAATGC(1/-1)
BsrI          448  CCAGT                ACTGG(1/-1)
BstAPI        133  GCAGCAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        375  GGATG                GGATG(2/0)
CfoI           39  GCGC                 GCG^C
DsaI           59  CCATGG               C^C[GA][CT]GG
EcoT14I        59  CCATGG               C^C[AT][AT]GG
FbaI          453  TGATCA               T^GATCA
FokI          375  GGATG                GGATG(9/13)
HaeII          38  GGCGCT               [GA]GCGC^[CT]
HhaI           39  GCGC                 GCG^C
HinP1I         39  GCGC                 G^CGC
HindIII       119  AAGCTT               A^AGCTT
HphI          275  TCACC                GGTGA(8/7)
MaeIII        218  GTGAC                ^GT[ACGT]AC
MspA1I        100  CAGCTG               C[AC]G^C[GT]G
NcoI           59  CCATGG               C^CATGG
PvuII         100  CAGCTG               CAG^CTG
SacI          106  GAGCTC               GAGCT^C
SfcI          414  CTACAG               C^T[GA][CT]AG
SphI          333  GCATGC               GCATG^C
SspI          538  AATATT               AAT^ATT
StyI           59  CCATGG               C^C[AT][AT]GG
Tsp45I        218  GTGAC                ^GT[GC]AC
XcmI           74  CCAGCCGCCTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          160  GAAATCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AspHI         106  GAGCTC               G[AT]GC[AT]^C
              485  GTGCTC              
BbvI          133  GCAGC                GCAGC(8/12)
              315  GCAGC               
BmyI          106  GAGCTC               G[AGT]GC[ACT]^C
              485  GTGCTC              
BseRI          51  CTCCTC               GAGGAG(10/8)
               54  CTCCTC              
BsiHKAI       106  GAGCTC               G[AT]GC[AT]^C
              485  GTGCTC              
Bsp1286I      106  GAGCTC               G[AGT]GC[ACT]^C
              485  GTGCTC              
BsrBI          48  CCGCTC               CCGCTC(-3/-3)
               69  CCGCTC              
BssKI          93  CCTGG                ^CC[ACGT]GG
              198  CCAGG               
BstNI          93  CCTGG                CC^[AT]GG
              198  CCAGG               
Cac8I          80  GCCTGC               GC[ACGT]^[ACGT]GC
              333  GCATGC              
Cfr13I         96  GGACC                G^G[ACGT]CC
              445  GGGCC               
EcoRII         93  CCTGG                ^CC[AT]GG
              198  CCAGG               
HaeIII        446  GGCC                 GG^CC
              558  GGCC                
MvaI           93  CCTGG                CC^[AT]GG
              198  CCAGG               
NlaIV         435  GGTGCC               GG[ACGT]^[ACGT]CC
              592  GGTTCC              
NspI          333  GCATGC               [GA]CATG^[CT]
              421  GCATGT              
PleI          525  GACTC                GAGTC(4/5)
              396  GAGTC               
Sau96I         96  GGACC                G^G[ACGT]CC
              445  GGGCC               
ScrFI          93  CCTGG                CC^[ACGT]GG
              198  CCAGG               
TfiI          322  GAATC                G^A[AT]TC
              626  GATTC               
TseI          133  GCAGC                G^C[AT]GC
              315  GCAGC               
TspRI         216  CAGTG                CA[GC]TG[ACGT][ACGT]^
              417  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

AciI        AluI        BpmI        BsiYI       BslI        
DdeI        DpnII       Fnu4HI      HinfI       ItaI        
MboI        MboII       MnlI        MseI        MwoI        
NdeII       NlaIII      Sau3AI      Tru9I       Tsp509I     
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