Restriction map for
pF1KB8399
(Linear) (Six-base) MAPSORT of: KIBB8399.seq Check: 9060 from: 1 to: 681
KIBB8399.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AleI CACnn'nnGTG
Cuts at: 0 402 681
Size: 402 279
ApaI G_GGCC'C
Cuts at: 0 40 681
Size: 40 641
AvaI C'yCGr_G
Cuts at: 0 32 45 227 359 681
Size: 32 13 182 132 322
Fragments arranged by size:
322 182 132 32 13
BanI G'GyrC_C
Cuts at: 0 90 198 232 252 681
Size: 90 108 34 20 429
Fragments arranged by size:
429 108 90 34 20
BanII G_rGCy'C
Cuts at: 0 40 358 681
Size: 40 318 323
Fragments arranged by size:
323 318 40
BbeI G_GCGC'C
Cuts at: 0 94 256 681
Size: 94 162 425
Fragments arranged by size:
425 162 94
BglI GCCn_nnn'nGGC
Cuts at: 0 370 681
Size: 370 311
BglII A'GATC_T
Cuts at: 0 655 681
Size: 655 26
Bme1580I G_kGCm'C
Cuts at: 0 40 107 203 235 436 681
Size: 40 67 96 32 201 245
Fragments arranged by size:
245 201 96 67 40 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 518 681
Size: 518 163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 681
Size: 583 98
BsaI GGTCTCn'nnnn_
Cuts at: 0 57 681
Size: 57 624
BsaHI Gr'CG_yC
Cuts at: 0 91 253 681
Size: 91 162 428
Fragments arranged by size:
428 162 91
BsaWI w'CCGG_w
Cuts at: 0 110 663 681
Size: 110 553 18
Fragments arranged by size:
553 110 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 340 370 681
Size: 340 30 311
Fragments arranged by size:
340 311 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 580 681
Size: 580 101
BseYI C'CCAG_C
Cuts at: 0 181 681
Size: 181 500
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 681
Size: 373 308
BsiEI CG_ry'CG
Cuts at: 0 191 681
Size: 191 490
BsiHKAI G_wGCw'C
Cuts at: 0 19 681
Size: 19 662
BsmBI CGTCTCn'nnnn_
Cuts at: 0 307 329 681
Size: 307 22 352
Fragments arranged by size:
352 307 22
Bsp1286I G_dGCh'C
Cuts at: 0 19 40 107 203 235 358 436 681
Size: 19 21 67 96 32 123 78 245
Fragments arranged by size:
245 123 96 78 67 32 21 19
BspEI T'CCGG_A
Cuts at: 0 663 681
Size: 663 18
BsrBI CCG'CTC
Cuts at: 0 29 146 681
Size: 29 117 535
Fragments arranged by size:
535 117 29
BsrDI GCAATG_nn'
Cuts at: 0 306 681
Size: 306 375
BsrFI r'CCGG_y
Cuts at: 0 235 681
Size: 235 446
BssSI C'ACGA_G
Cuts at: 0 636 681
Size: 636 45
BstEII G'GTnAC_C
Cuts at: 0 304 325 681
Size: 304 21 356
Fragments arranged by size:
356 304 21
BstXI CCAn_nnnn'nTGG
Cuts at: 0 301 453 681
Size: 301 152 228
Fragments arranged by size:
301 228 152
BstYI r'GATC_y
Cuts at: 0 655 681
Size: 655 26
BtgI C'CryG_G
Cuts at: 0 128 294 681
Size: 128 166 387
Fragments arranged by size:
387 166 128
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 378 681
Size: 378 303
EaeI y'GGCC_r
Cuts at: 0 188 681
Size: 188 493
EagI C'GGCC_G
Cuts at: 0 188 681
Size: 188 493
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 278 388 681
Size: 278 110 293
Fragments arranged by size:
293 278 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 681
Size: 583 98
EcoO109I rG'GnC_Cy
Cuts at: 0 36 37 681
Size: 36 1 644
Fragments arranged by size:
644 36 1
FspI TGC'GCA
Cuts at: 0 139 681
Size: 139 542
HaeII r_GCGC'y
Cuts at: 0 71 94 256 681
Size: 71 23 162 425
Fragments arranged by size:
425 162 71 23
KasI G'GCGC_C
Cuts at: 0 90 252 681
Size: 90 162 429
Fragments arranged by size:
429 162 90
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 681
Size: 302 379
MslI CAynn'nnrTG
Cuts at: 0 172 299 402 681
Size: 172 127 103 279
Fragments arranged by size:
279 172 127 103
NarI GG'CG_CC
Cuts at: 0 91 253 681
Size: 91 162 428
Fragments arranged by size:
428 162 91
NcoI C'CATG_G
Cuts at: 0 128 294 681
Size: 128 166 387
Fragments arranged by size:
387 166 128
PsiI TTA'TAA
Cuts at: 0 484 681
Size: 484 197
PspOMI G'GGCC_C
Cuts at: 0 36 681
Size: 36 645
PstI C_TGCA'G
Cuts at: 0 578 681
Size: 578 103
SfcI C'TryA_G
Cuts at: 0 574 681
Size: 574 107
SfoI GGC'GCC
Cuts at: 0 92 254 681
Size: 92 162 427
Fragments arranged by size:
427 162 92
SgrAI Cr'CCGG_yG
Cuts at: 0 235 681
Size: 235 446
SmaI CCC'GGG
Cuts at: 0 34 47 229 681
Size: 34 13 182 452
Fragments arranged by size:
452 182 34 13
StyI C'CwwG_G
Cuts at: 0 128 294 681
Size: 128 166 387
Fragments arranged by size:
387 166 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 138 414 681
Size: 138 276 267
Fragments arranged by size:
276 267 138
XmaI C'CCGG_G
Cuts at: 0 32 45 227 681
Size: 32 13 182 454
Fragments arranged by size:
454 182 32 13
Enzymes that do cut:
AleI ApaI AvaI BanI BanII BbeI BglI BglII
Bme1580I BplI BpmI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BsrBI
BsrDI BsrFI BssSI BstEII BstXI BstYI BtgI BtgZI
EaeI EagI EciI Eco57MI EcoO109I FspI HaeII KasI
MmeI MslI NarI NcoI PsiI PspOMI PstI SfcI
SfoI SgrAI SmaI StyI TaqII XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BcgI BciVI BclI BfrBI BlpI BmgBI BmrI BmtI
Bpu10I BpuEI BsaAI BsaBI BsiWI BsmI BspHI BspMI
BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII DrdI EarI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MscI MspA1I NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmnI ZraI