Restriction map for pF1KB8398
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AgeI        Aor51HI     ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AviII       AvrII       BbrPI       BbsI        BbvCI       
BciVI       BfrI        BlnI        BpuAI       BsaAI       
BsiWI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Cfr10I      
ClaI        CpoI        DraI        DrdI        EarI        
Eco47III    EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HpaI        KpnI        
Ksp632I     KspI        MfeI        MluI        MseI        
MunI        NaeI        NdeI        NgoMIV      NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        Tru9I       Tsp509I     Tth111I     
TthHB8I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AcyI          224  GGCGCC               G[GA]^CG[CT]C
AfaI         1264  GTAC                 GT^AC
AflIII       1427  ACGTGT               A^C[GA][CT]GT
AhdI          694  GACAGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        487  GTGCAC               G^TGCAC
ApaI         1758  GGGCCC               GGGCC^C
ApaLI         487  GTGCAC               G^TGCAC
AspEI         694  GACAGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI          224  GGCGCC               GGCGC^C
BcgI          365  GCAGCAAGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI           80  TGATCA               T^GATCA
BlpI         1284  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1284  GCTCAGC              GC^T[ACGT]AGC
BsaHI         224  GGCGCC               G[GA]^CG[CT]C
BsaI          169  GAGACC               GGTCTC(1/5)
BsgI         1050  GTGCAG               GTGCAG(16/14)
BsmBI         477  CGTCTC               CGTCTC(1/5)
BspMI         143  ACCTGC               ACCTGC(4/8)
BsrBI         427  GAGCGG               CCGCTC(-3/-3)
BsrDI         336  CATTGC               GCAATG(2/0)
BstAPI        138  GCACAACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        1284  GCTCAGC              GC^T[ACGT]AGC
DraIII       1073  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          992  CCATGG               C^C[GA][CT]GG
Eam1105I      694  GACAGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI           80  TGATCA               T^GATCA
HgaI         1417  GACGC                GACGC(5/10)
Hin1I         224  GGCGCC               G[GA]^CG[CT]C
HincII        907  GTCAAC               GT[CT]^[GA]AC
HindII        907  GTCAAC               GT[CT]^[GA]AC
HindIII      1469  AAGCTT               A^AGCTT
KasI          224  GGCGCC               G^GCGCC
MaeII        1427  ACGT                 A^CGT
NarI          224  GGCGCC               GG^CGCC
NcoI          992  CCATGG               C^CATGG
NheI           54  GCTAGC               G^CTAGC
NspI         1068  GCATGC               [GA]CATG^[CT]
PflMI         893  CCAGTCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1271  AGGACCC              [GA]G^G[AT]CC[CT]
RsaI         1264  GTAC                 GT^AC
SexAI        1074  ACCTGGT              A^CC[AT]GGT
SfoI          224  GGCGCC               GGC^GCC
SmaI         1601  CCCGGG               CCC^GGG
SphI         1068  GCATGC               GCATG^C
TfiI         1399  GAATC                G^A[AT]TC
Van91I        893  CCAGTCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1091  CCACCCCTGGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1601  CCCGGG               C^CCGGG
XmaI         1601  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccIII        248  TCCGGA               T^CCGGA
             1278  TCCGGA              
AocI         1616  CCTCAGG              CC^T[ACGT]AGG
             1773  CCTGAGG             
AvaII         701  GGTCC                G^G[AT]CC
             1272  GGACC               
BalI          233  TGGCCA               TGG^CCA
              890  TGGCCA              
BamHI        1443  GGATCC               G^GATCC
             1791  GGATCC              
BglI          988  GCCTCCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1677  GCCTGAACGGC         
BglII         276  AGATCT               A^GATCT
              522  AGATCT              
BsaBI         300  GATTGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              372  GATCGTGATC          
BseAI         248  TCCGGA               T^CCGGA
             1278  TCCGGA              
BsiEI          44  CGGCCG               CG[GA][CT]^CG
               61  CGGCCG              
BspEI         248  TCCGGA               T^CCGGA
             1278  TCCGGA              
BstXI         923  CCACCCGAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1188  CCATCGCCCTGG        
Bsu36I       1616  CCTCAGG              CC^T[ACGT]AGG
             1773  CCTGAGG             
EagI           44  CGGCCG               C^GGCCG
               61  CGGCCG              
EclXI          44  CGGCCG               C^GGCCG
               61  CGGCCG              
Eco52I         44  CGGCCG               C^GGCCG
               61  CGGCCG              
Eco81I       1616  CCTCAGG              CC^T[ACGT]AGG
             1773  CCTGAGG             
MamI          300  GATTGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              372  GATCGTGATC          
MluNI         233  TGGCCA               TGG^CCA
              890  TGGCCA              
MroI          248  TCCGGA               T^CCGGA
             1278  TCCGGA              
MscI          233  TGGCCA               TGG^CCA
              890  TGGCCA              
PstI          616  CTGCAG               CTGCA^G
             1253  CTGCAG              
SfcI          616  CTGCAG               C^T[GA][CT]AG
             1253  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AlwNI       
AspHI       AvaI        BanI        BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaJI       
BsaWI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PvuII       SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        StyI        TseI        Tsp45I      
TspRI       XhoII       
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