Restriction map for pF1KB8397
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BspMI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        HphI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MslI        MspA1I      
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
StuI        SwaI        Tsp45I      Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccI          979  GTAGAC               GT^[AC][GT]AC
AccIII        790  TCCGGA               T^CCGGA
AflIII        742  ACACGT               A^C[GA][CT]GT
Asp700I       909  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII         993  CCTAGG               C^CTAGG
BalI          846  TGGCCA               TGG^CCA
BamHI        1149  GGATCC               G^GATCC
BanI          939  GGCACC               G^G[CT][GA]CC
BanII        1135  GGGCTC               G[GA]GC[CT]^C
BbvI          898  GCAGC                GCAGC(8/12)
BlnI          993  CCTAGG               C^CTAGG
BmyI         1135  GGGCTC               G[AGT]GC[ACT]^C
BsaWI         790  TCCGGA               [AT]^CCGG[AT]
BseAI         790  TCCGGA               T^CCGGA
BseRI         340  GAGGAG               GAGGAG(10/8)
BsgI          755  GTGCAG               GTGCAG(16/14)
Bsp1286I     1135  GGGCTC               G[AGT]GC[ACT]^C
BspEI         790  TCCGGA               T^CCGGA
BsrBI         718  GAGCGG               CCGCTC(-3/-3)
BsrDI        1124  CATTGC               GCAATG(2/0)
BstEII       1028  GGTTACC              G^GT[ACGT]ACC
BstPI        1028  GGTTACC              G^GT[ACGT]ACC
BstYI        1149  GGATCC               [GA]^GATC[CT]
DraI         1264  TTTAAA               TTT^AAA
DraII         989  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I      989  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      1028  GGTTACC              G^GT[ACGT]ACC
FauI          720  GCGGG                CCCGC(4/6)
Fnu4HI        898  GCAGC                GC^[ACGT]GC
HapII         791  CCGG                 C^CGG
HindIII      1249  AAGCTT               A^AGCTT
HpaII         791  CCGG                 C^CGG
ItaI          898  GCAGC                GC^[ACGT]GC
MaeII         744  ACGT                 A^CGT
MflI         1149  GGATCC               [GA]^GATC[CT]
MluNI         846  TGGCCA               TGG^CCA
MroI          790  TCCGGA               T^CCGGA
MscI          846  TGGCCA               TGG^CCA
MspI          791  CCGG                 C^CGG
NspI         1373  GCATGC               [GA]CATG^[CT]
PstI          325  CTGCAG               CTGCA^G
SphI         1373  GCATGC               GCATG^C
TseI          898  GCAGC                G^C[AT]GC
XhoII        1149  GGATCC               [GA]^GATC[CT]
XmnI          909  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AciI          738  CCGC                 CCGC(-3/-1)
              720  GCGG                
AcsI         1090  AAATTT               [GA]^AATT[CT]
             1312  GAATTT              
AfaI          804  GTAC                 GT^AC
             1255  GTAC                
ApoI         1090  AAATTT               [GA]^AATT[CT]
             1312  GAATTT              
AseI         1068  ATTAAT               AT^TAAT
             1074  ATTAAT              
AsnI         1068  ATTAAT               AT^TAAT
             1074  ATTAAT              
BssKI         306  CCAGG                ^CC[ACGT]GG
             1044  CCAGG               
BstNI         306  CCAGG                CC^[AT]GG
             1044  CCAGG               
Cac8I         692  GCGAGC               GC[ACGT]^[ACGT]GC
             1373  GCATGC              
EaeI          846  TGGCCA               [CT]^GGCC[GA]
              969  TGGCCG              
EcoRII        306  CCAGG                ^CC[AT]GG
             1044  CCAGG               
HinfI         531  GATTC                G^A[ACGT]TC
              825  GAATC               
MvaI          306  CCAGG                CC^[AT]GG
             1044  CCAGG               
MwoI          301  GCTATCCAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1128  GCTTTCAGGGC         
PshBI        1068  ATTAAT               AT^TAAT
             1074  ATTAAT              
RsaI          804  GTAC                 GT^AC
             1255  GTAC                
ScrFI         306  CCAGG                CC^[ACGT]GG
             1044  CCAGG               
SfcI          311  CTGTAG               C^T[GA][CT]AG
              325  CTGCAG              
SspI          635  AATATT               AAT^ATT
             1071  AATATT              
TfiI          531  GATTC                G^A[AT]TC
              825  GAATC               
TspRI         164  CAGTG                CA[GC]TG[ACGT][ACGT]^
              232  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AluI        AlwI        AvaII       BbsI        BfaI        
BpmI        BpuAI       BsaJI       BsmAI       BsrI        
BstF5I      Cfr13I      DdeI        DpnII       EarI        
EcoT14I     FokI        HaeIII      Ksp632I     MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
SfaNI       SmlI        StyI        TaqI        Tru9I       
Tsp509I     TthHB8I     
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