Restriction map for pF1KB8397
 (Circular) (Six-base) MAPSORT of: pF1KB8397.seq  Check: 9914  from: 1  to: 4499



pF1KB8397  4499 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:28  ..

AccI GT'mk_AC

Cuts at:   1088    1537    1088 
   Size:        449    4050
 
Acc65I G'GTAC_C

Cuts at:   1515    1515 
   Size:       4499
 
AclI AA'CG_TT

Cuts at:   1493    4339    1493 
   Size:       2846    1653
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    664    1202    2324    2756    3109    3771     664 
   Size:        538    1122     432     353     662    1392

  Fragments arranged by size: 

                1392    1122     662     538     432     353
 
AflIII A'CryG_T

Cuts at:    850    1851    3651    3932     850 
   Size:       1001    1800     281    1417

  Fragments arranged by size: 

                1800    1417    1001     281
 
AlwNI CAG_nnn'CTG

Cuts at:   1804    3242    1804 
   Size:       1438    3061
 
ApaLI G'TGCA_C

Cuts at:   3337    3337 
   Size:       4499
 
ApoI r'AATT_y

Cuts at:   1198    1420    1503    3852    4108    1198 
   Size:        222      83    2349     256    1589

  Fragments arranged by size: 

                2349    1589     256     222      83
 
AseI AT'TA_AT

Cuts at:     20    1177    1183      20 
   Size:       1157       6    3336

  Fragments arranged by size: 

                3336    1157       6
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4499
 
AvaI C'yCGr_G

Cuts at:   1519    1519 
   Size:       4499
 
AvrII C'CTAG_G

Cuts at:   1101    1101 
   Size:       4499
 
BamHI G'GATC_C

Cuts at:   1257    1524    1257 
   Size:        267    4232
 
BanI G'GyrC_C

Cuts at:   1047    1515    1929    2177    2212    1047 
   Size:        468     414     248      35    3334

  Fragments arranged by size: 

                3334     468     414     248      35
 
BanII G_rGCy'C

Cuts at:   1247    1513    2543    1247 
   Size:        266    1030    3203

  Fragments arranged by size: 

                3203    1030     266
 
BbeI G_GCGC'C

Cuts at:   1933    2181    1933 
   Size:        248    4251
 
BbsI GAAGACnn'nnnn_

Cuts at:    202    1024    1196     202 
   Size:        822     172    3505

  Fragments arranged by size: 

                3505     822     172
 
BciVI GTATCCnnnnn_n'

Cuts at:   2397    3453    4499    2397 
   Size:       1056    1046    2397

  Fragments arranged by size: 

                2397    1056    1046
 
BclI T'GATC_A

Cuts at:   2019    2019 
   Size:       4499
 
BglII A'GATC_T

Cuts at:   2014    4051    2014 
   Size:       2037    2462
 
BlpI GC'TnA_GC

Cuts at:   1611    1611 
   Size:       4499
 
Bme1580I G_kGCm'C

Cuts at:   2124    2217    3341    2124 
   Size:         93    1124    3282

  Fragments arranged by size: 

                3282    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1711    1792    2127    4271    1711 
   Size:         81     335    2144    1939

  Fragments arranged by size: 

                2144    1939     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2866    2866 
   Size:       4499
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    192     322     365    1019    1346     192 
   Size:        130      43     654     327    3345

  Fragments arranged by size: 

                3345     654     327     130      43
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    218     907    1364    1675    2554    3268    3566     218 
   Size:        689     457     311     879     714     298    1151

  Fragments arranged by size: 

                1151     879     714     689     457     311     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4203    4203 
   Size:       4499
 
BsaAI yAC'GTr

Cuts at:   1852    1852 
   Size:       4499
 
BsaBI GATnn'nnATC

Cuts at:      6    2013    4050       6 
   Size:       2007    2037     455

  Fragments arranged by size: 

                2037    2007     455
 
BsaHI Gr'CG_yC

Cuts at:   1930    2178    2880    4376    1930 
   Size:        248     702    1496    2053

  Fragments arranged by size: 

                2053    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    898    1688    2209    2887    3298    3445    3719     898 
   Size:        790     521     678     411     147     274    1678

  Fragments arranged by size: 

                1678     790     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    463     463 
   Size:       4499
 
BseYI C'CCAG_C

Cuts at:   1485    1924    2682    3347    3755    1485 
   Size:        439     758     665     408    2229

  Fragments arranged by size: 

                2229     758     665     439     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    884     884 
   Size:       4499
 
BsiEI CG_ry'CG

Cuts at:    104    2041    2087    3317     104 
   Size:       1937      46    1230    1286

  Fragments arranged by size: 

                1937    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   1513    2291    2481    3341    1513 
   Size:        778     190     860    2671

  Fragments arranged by size: 

                2671     860     778     190
 
Bsp1286I G_dGCh'C

Cuts at:   1247    1513    2124    2217    2291    2481    2543    3341    1247 
   Size:        266     611      93      74     190      62     798    2405

  Fragments arranged by size: 

                2405     798     611     266     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:    898     898 
   Size:       4499
 
BspHI T'CATG_A

Cuts at:   2931    2931 
   Size:       4499
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1549    1763    2065    2446    1549 
   Size:        214     302     381    3602

  Fragments arranged by size: 

                3602     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     828    2791    2845    4329    4494      50 
   Size:        778    1963      54    1484     165      55

  Fragments arranged by size: 

                1963    1484     778     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1231    2411    1231 
   Size:       1180    3319
 
BsrFI r'CCGG_y

Cuts at:   2497    2678    2497 
   Size:        181    4318
 
BssSI C'ACGA_G

Cuts at:   2770    3478    2770 
   Size:        708    3791
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1876    1876 
   Size:       4499
 
BstBI TT'CG_AA

Cuts at:   2860    2860 
   Size:       4499
 
BstEII G'GTnAC_C

Cuts at:   1136    1136 
   Size:       4499
 
BstYI r'GATC_y

Cuts at:      1    1257    1524    1991    2014    2349    2595    2901    2913 
   Size:       1256     267     467      23     335     246     306      12

Cuts at:   2913    2999    4051       1 
   Size:         86    1052     449

  Fragments arranged by size: 

                1256    1052     467     449     335     306     267     246
                  86      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    128    2449    2630    2793     128 
   Size:       2321     181     163    1834

  Fragments arranged by size: 

                2321    1834     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1742    1742 
   Size:       4499
 
DraI TTT'AAA

Cuts at:   1374    1498    1374 
   Size:        124    4375
 
DrdI GACnn_nn'nnGTC

Cuts at:   2205    3549    2205 
   Size:       1344    3155
 
EaeI y'GGCC_r

Cuts at:    954    1077    2084    2258    2649    2676    4021     954 
   Size:        123    1007     174     391      27    1345    1432

  Fragments arranged by size: 

                1432    1345    1007     391     174     123      27
 
EagI C'GGCC_G

Cuts at:   2084    2084 
   Size:       4499
 
EarI CTCTTCn'nnn_

Cuts at:    142     440     494    1221    2522    2732     142 
   Size:        298      54     727    1301     210    1909

  Fragments arranged by size: 

                1909    1301     727     298     210      54
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2707    3449    3595    4309    2707 
   Size:        742     146     714    2897

  Fragments arranged by size: 

                2897     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1511    1511 
   Size:       4499
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    192     322     365     664    1019    1202    1346    2324    2756 
   Size:        130      43     299     355     183     144     978     432

Cuts at:   2756    3109    3771     192 
   Size:        353     662     920

  Fragments arranged by size: 

                 978     920     662     432     355     353     299     183
                 144     130      43
 
EcoO109I rG'GnC_Cy

Cuts at:   1098    1638    1098 
   Size:        540    3959
 
EcoRI G'AATT_C

Cuts at:   1503    1503 
   Size:       4499
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2518    2518 
   Size:       4499
 
FspI TGC'GCA

Cuts at:   2280    2280 
   Size:       4499
 
HaeII r_GCGC'y

Cuts at:   1933    2181    3411    4182    1933 
   Size:        248    1230     771    2250

  Fragments arranged by size: 

                2250    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1213    1242    1245    1274    2341    2371    2373    2403    1213 
   Size:         29       3      29    1067      30       2      30    3309

  Fragments arranged by size: 

                3309    1067      30      30      29      29       3       2
            
 
HincII GTy'rAC

Cuts at:   1538    1538 
   Size:       4499
 
HindIII A'AGCT_T

Cuts at:   1357    1357 
   Size:       4499
 
KasI G'GCGC_C

Cuts at:   1929    2177    1929 
   Size:        248    4251
 
KpnI G_GTAC'C

Cuts at:   1519    1519 
   Size:       4499
 
MluI A'CGCG_T

Cuts at:   3932    3932 
   Size:       4499
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    493     908    3258    3442     493 
   Size:        415    2350     184    1550

  Fragments arranged by size: 

                2350    1550     415     184
 
MscI TGG'CCA

Cuts at:    956    2260     956 
   Size:       1304    3195
 
MslI CAynn'nnrTG

Cuts at:   2488    2615    2488 
   Size:        127    4372
 
MspA1I CmG'CkG

Cuts at:   1610    1924    2284    3068    3313    1610 
   Size:        314     360     784     245    2796

  Fragments arranged by size: 

                2796     784     360     314     245
 
NaeI GCC'GGC

Cuts at:   2680    2680 
   Size:       4499
 
NarI GG'CG_CC

Cuts at:   1930    2178    1930 
   Size:        248    4251
 
NgoMIV G'CCGG_C

Cuts at:   2678    2678 
   Size:       4499
 
NspI r_CATG'y

Cuts at:   1485    1552    2052    3655    1485 
   Size:         67     500    1603    2329

  Fragments arranged by size: 

                2329    1603     500      67
 
PciI A'CATG_T

Cuts at:   3651    3651 
   Size:       4499
 
PmeI GTTT'AAAC

Cuts at:   1498    1498 
   Size:       4499
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    817     849    2945    2977     817 
   Size:         32    2096      32    2339

  Fragments arranged by size: 

                2339    2096      32      32
 
PsiI TTA'TAA

Cuts at:    325     325 
   Size:       4499
 
PstI C_TGCA'G

Cuts at:    437    1546    2231     437 
   Size:       1109     685    2705

  Fragments arranged by size: 

                2705    1109     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4499
 
PvuII CAG'CTG

Cuts at:   1924    2284    1924 
   Size:        360    4139
 
RsrII CG'GwC_CG

Cuts at:   2694    2694 
   Size:       4499
 
SacI G_AGCT'C

Cuts at:   1513    1513 
   Size:       4499
 
SalI G'TCGA_C

Cuts at:   1536    1536 
   Size:       4499
 
SapI GCTCTTCn'nnn_

Cuts at:   2522    2732    2522 
   Size:        210    4289
 
SbfI CC_TGCA'GG

Cuts at:   1546    1546 
   Size:       4499
 
ScaI AGT'ACT

Cuts at:   4016    4016 
   Size:       4499
 
SfcI C'TryA_G

Cuts at:     33     419     433    1542    2227    3195    3386      33 
   Size:        386      14    1109     685     968     191    1146

  Fragments arranged by size: 

                1146    1109     968     685     386     191      14
 
SfoI GGC'GCC

Cuts at:   1931    2179    1931 
   Size:        248    4251
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4499
 
SmaI CCC'GGG

Cuts at:   1521    1521 
   Size:       4499
 
SmlI C'TyrA_G

Cuts at:    233     922    1379    1654    2569    3283    3545     233 
   Size:        689     457     275     915     714     262    1187

  Fragments arranged by size: 

                1187     915     714     689     457     275     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4499
 
SphI G_CATG'C

Cuts at:   1485    1552    2052    1485 
   Size:         67     500    3932

  Fragments arranged by size: 

                3932     500      67
 
SspI AAT'ATT

Cuts at:    745    1181     745 
   Size:        436    4063
 
StyI C'CwwG_G

Cuts at:    881    1101    1272    1633    1987     881 
   Size:        220     171     361     354    3393

  Fragments arranged by size: 

                3393     361     354     220     171
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2082    2215    2674    2883    2082 
   Size:        133     459     209    3698

  Fragments arranged by size: 

                3698     459     209     133
 
TatI w'GTAC_w

Cuts at:   4014    4014 
   Size:       4499
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    705     737     893     925    4016    4048     705 
   Size:         32     156      32    3091      32    1156

  Fragments arranged by size: 

                3091    1156     156      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2296    2296 
   Size:       4499
 
XbaI T'CTAG_A

Cuts at:   1530    1530 
   Size:       4499
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1994    1994 
   Size:       4499
 
XmaI C'CCGG_G

Cuts at:   1519    1519 
   Size:       4499
 
XmnI GAAnn'nnTTC

Cuts at:   1021    1021 
   Size:       4499
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstYI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI     PmeI     PpiI
     PsiI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     SspI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     ApaI     AscI     BaeI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI   Bpu10I    BsaXI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI   DraIII
    EcoNI    EcoRV     FseI    FspAI     HpaI     MfeI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     StuI     SwaI     XhoI
     ZraI
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