Restriction map for pF1KB8396
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AgeI        Alw44I      AlwNI       AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AviII       AvrII       
BalI        BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaI        BsgI        BsiWI       
BsmAI       BsmBI       BsmI        Bsp1407I    BspDI       
BspHI       BspLU11I    BspMI       BsrDI       BsrGI       
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      DrdI        Eco47III    
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        MaeI        
MamI        MfeI        MluNI       MscI        MseI        
MunI        NcoI        NdeI        NheI        NruI        
NsiI        NspV        PacI        PflMI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tru9I       
Van91I      XbaI        XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccIII        763  TCCGGA               T^CCGGA
AfaI          758  GTAC                 GT^AC
AflIII        284  ACGCGT               A^C[GA][CT]GT
AhdI         1186  GACCTTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        1186  GACCTTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          832  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         561  GGATCC               G^GATCC
BanI          457  GGCGCC               G^G[CT][GA]CC
BbeI          457  GGCGCC               GGCGC^C
BbrPI        1219  CACGTG               CAC^GTG
BbsI          574  GTCTTC               GAAGAC(2/6)
BpuAI         574  GTCTTC               GAAGAC(2/6)
BsaWI         763  TCCGGA               [AT]^CCGG[AT]
BseAI         763  TCCGGA               T^CCGGA
BspEI         763  TCCGGA               T^CCGGA
BssHII        381  GCGCGC               G^CGCGC
BstYI         561  GGATCC               [GA]^GATC[CT]
Eam1105I     1186  GACCTTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
KasI          457  GGCGCC               G^GCGCC
KspI          411  CCGCGG               CCGC^GG
MflI          561  GGATCC               [GA]^GATC[CT]
MluI          284  ACGCGT               A^CGCGT
MroI          763  TCCGGA               T^CCGGA
NarI          457  GGCGCC               GG^CGCC
NotI          719  GCGGCCGC             GC^GGCCGC
NspI          344  GCATGT               [GA]CATG^[CT]
PaeR7I         55  CTCGAG               C^TCGAG
PflFI         832  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1219  CACGTG               CAC^GTG
PmlI         1219  CACGTG               CAC^GTG
PpuMI         924  GGGTCCC              [GA]G^G[AT]CC[CT]
PstI          400  CTGCAG               CTGCA^G
RsaI          758  GTAC                 GT^AC
SacI          532  GAGCTC               GAGCT^C
SacII         411  CCGCGG               CCGC^GG
SfoI          457  GGCGCC               GGC^GCC
TfiI          784  GATTC                G^A[AT]TC
Tth111I       832  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI           55  CTCGAG               C^TCGAG
XhoII         561  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AcyI          457  GGCGCC               G[GA]^CG[CT]C
             1059  GGCGTC              
ApaI          645  GGGCCC               GGGCC^C
              689  GGGCCC              
BsaAI        1012  TACGTG               [CT]AC^GT[GA]
             1219  CACGTG              
BsaHI         457  GGCGCC               G[GA]^CG[CT]C
             1059  GGCGTC              
BsrBI        1072  CCGCTC               CCGCTC(-3/-3)
              351  GAGCGG              
BstAPI         32  GCATGATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1353  GCAGCAGTTGC         
EarI          805  GAAGAG               CTCTTC(1/4)
              817  GAAGAG              
EcoT14I       872  CCAAGG               C^C[AT][AT]GG
             1253  CCAAGG              
Hin1I         457  GGCGCC               G[GA]^CG[CT]C
             1059  GGCGTC              
Ksp632I       805  GAAGAG               CTCTTC(1/4)
              817  GAAGAG              
NaeI          454  GCCGGC               GCC^GGC
              502  GCCGGC              
NgoMIV        454  GCCGGC               G^CCGGC
              502  GCCGGC              
SfcI           70  CTACAG               C^T[GA][CT]AG
              400  CTGCAG              
StyI          872  CCAAGG               C^C[AT][AT]GG
             1253  CCAAGG              
Tsp509I      1337  AATT                 ^AATT
             1371  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AspHI       
AvaI        AvaII       BanII       BbvI        BcnI        
BglI        BmyI        BpmI        BsaJI       BseRI       
BsiEI       BsiHKAI     BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EagI        EclXI       Eco52I      
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MnlI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        Sau3AI      Sau96I      ScrFI       SfaNI       
SmaI        SmlI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     XmaCI       XmaI        
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