Restriction map for
pF1KB7896
(Circular) (Six-base) MAPSORT of: pF1KB7896.seq Check: 132 from: 1 to: 5087
pF1KB7896 5087 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 2125 2125
Size: 5087
Acc65I G'GTAC_C
Cuts at: 2103 2103
Size: 5087
AclI AA'CG_TT
Cuts at: 4927 4927
Size: 5087
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 220 231 437 1011 1029 1179 1347 1431
Size: 61 11 206 574 18 150 168 84
Cuts at: 1431 1683 1767 1851 2912 3344 4091 4359 159
Size: 252 84 84 1061 432 747 268 887
Fragments arranged by size:
1061 887 747 574 432 268 252 206
168 150 84 84 84 61 18 11
AflIII A'CryG_T
Cuts at: 682 2439 3543 4520 682
Size: 1757 1104 977 1249
Fragments arranged by size:
1757 1249 1104 977
AgeI A'CCGG_T
Cuts at: 3475 3475
Size: 5087
AhdI GACnn_n'nnGTC
Cuts at: 382 382
Size: 5087
AleI CACnn'nnGTG
Cuts at: 559 986 559
Size: 427 4660
AlwNI CAG_nnn'CTG
Cuts at: 1140 2021 2392 3959 1140
Size: 881 371 1567 2268
Fragments arranged by size:
2268 1567 881 371
ApaLI G'TGCA_C
Cuts at: 3857 3857
Size: 5087
ApoI r'AATT_y
Cuts at: 2091 4440 4696 2091
Size: 2349 256 2482
Fragments arranged by size:
2482 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5087
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5087
AvaI C'yCGr_G
Cuts at: 2107 2107
Size: 5087
BamHI G'GATC_C
Cuts at: 1733 1985 2112 1733
Size: 252 127 4708
Fragments arranged by size:
4708 252 127
BanI G'GyrC_C
Cuts at: 2103 2517 2765 2800 2103
Size: 414 248 35 4390
Fragments arranged by size:
4390 414 248 35
BanII G_rGCy'C
Cuts at: 1795 2101 3131 1795
Size: 306 1030 3751
Fragments arranged by size:
3751 1030 306
BbeI G_GCGC'C
Cuts at: 2521 2769 2521
Size: 248 4839
BbsI GAAGACnn'nnnn_
Cuts at: 571 571
Size: 5087
BciVI GTATCCnnnnn_n'
Cuts at: 2985 3746 5087 2985
Size: 761 1341 2985
Fragments arranged by size:
2985 1341 761
BclI T'GATC_A
Cuts at: 2607 2607
Size: 5087
BfrBI ATG'CAT
Cuts at: 2011 2011
Size: 5087
BglII A'GATC_T
Cuts at: 2602 4639 2602
Size: 2037 3050
BlpI GC'TnA_GC
Cuts at: 786 1885 2199 786
Size: 1099 314 3674
Fragments arranged by size:
3674 1099 314
Bme1580I G_kGCm'C
Cuts at: 2712 2805 3861 2712
Size: 93 1056 3938
Fragments arranged by size:
3938 1056 93
BmgBI CAC'GTC
Cuts at: 162 162
Size: 5087
BmrI ACTGGGnnnn_n'
Cuts at: 513 911 1163 1667 1919 2299 2380 2715 4859
Size: 398 252 504 252 380 81 335 2144
Cuts at: 4859 513
Size: 741
Fragments arranged by size:
2144 741 504 398 380 335 252 252
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3454 3454
Size: 5087
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 598 2099 325
Size: 273 1501 3313
Fragments arranged by size:
3313 1501 273
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2263 3142 3634 3932 2263
Size: 879 492 298 3418
Fragments arranged by size:
3418 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 264 819 4791 264
Size: 555 3972 560
Fragments arranged by size:
3972 560 555
BsaAI yAC'GTr
Cuts at: 685 2440 685
Size: 1755 3332
BsaBI GATnn'nnATC
Cuts at: 6 2601 4638 6
Size: 2595 2037 455
Fragments arranged by size:
2595 2037 455
BsaHI Gr'CG_yC
Cuts at: 568 2518 2766 3468 4964 568
Size: 1950 248 702 1496 691
Fragments arranged by size:
1950 1496 702 691 248
BsaWI w'CCGG_w
Cuts at: 806 860 1979 2276 2797 3475 3749 3896 4307
Size: 54 1119 297 521 678 274 147 411
Cuts at: 4307 806
Size: 1586
Fragments arranged by size:
1586 1119 678 521 411 297 274 147
54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2032 2062 2032
Size: 30 5057
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 1449 1869 2037 199
Size: 1250 420 168 3249
Fragments arranged by size:
3249 1250 420 168
BseYI C'CCAG_C
Cuts at: 1133 2512 3270 3847 4343 1133
Size: 1379 758 577 496 1877
Fragments arranged by size:
1877 1379 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1110 1530 1110
Size: 420 4667
BsiEI CG_ry'CG
Cuts at: 104 2629 2675 3883 104
Size: 2525 46 1208 1308
Fragments arranged by size:
2525 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1375 1543 1795 1963 2101 2879 3069 3861 1375
Size: 168 252 168 138 778 190 792 2601
Fragments arranged by size:
2601 792 778 252 190 168 168 138
BsmI GAATG_Cn'
Cuts at: 766 850 1009 1597 1612 1681 766
Size: 84 159 588 15 69 4172
Fragments arranged by size:
4172 588 159 84 69 15
BsmBI CGTCTCn'nnnn_
Cuts at: 132 132
Size: 5087
Bsp1286I G_dGCh'C
Cuts at: 1375 1543 1795 1963 2101 2712 2805 2879 3069
Size: 168 252 168 138 611 93 74 190
Cuts at: 3069 3131 3861 1375
Size: 62 730 2601
Fragments arranged by size:
2601 730 611 252 190 168 168 138
93 74 62
BspEI T'CCGG_A
Cuts at: 860 4307 860
Size: 3447 1640
BspHI T'CATG_A
Cuts at: 4263 4263
Size: 5087
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2137 2351 2653 3034 2137
Size: 214 302 381 4190
Fragments arranged by size:
4190 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1479 3379 3433 4917 5082 50
Size: 1429 1900 54 1484 165 55
Fragments arranged by size:
1900 1484 1429 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1602 1854 2999 1602
Size: 252 1145 3690
Fragments arranged by size:
3690 1145 252
BsrFI r'CCGG_y
Cuts at: 543 3085 3266 3475 543
Size: 2542 181 209 2155
Fragments arranged by size:
2542 2155 209 181
BssSI C'ACGA_G
Cuts at: 1054 3358 3716 1054
Size: 2304 358 2425
Fragments arranged by size:
2425 2304 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1601 2464 1601
Size: 863 4224
BstBI TT'CG_AA
Cuts at: 3448 3448
Size: 5087
BstEII G'GTnAC_C
Cuts at: 275 275
Size: 5087
BstXI CCAn_nnnn'nTGG
Cuts at: 1267 1267
Size: 5087
BstYI r'GATC_y
Cuts at: 1 1733 1985 2112 2579 2602 2937 3183 4195
Size: 1732 252 127 467 23 335 246 1012
Cuts at: 4195 4281 4293 4639 1
Size: 86 12 346 449
Fragments arranged by size:
1732 1012 467 449 346 335 252 246
127 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3037 3218 3381 3037
Size: 181 163 4743
Fragments arranged by size:
4743 181 163
BtsI GCAGTG_nn'
Cuts at: 545 589 1098 2330 545
Size: 44 509 1232 3302
Fragments arranged by size:
3302 1232 509 44
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1416 1451 1668 1703 1836 1871 1416
Size: 35 217 35 133 35 4632
Fragments arranged by size:
4632 217 133 35 35 35
DraI TTT'AAA
Cuts at: 2086 2086
Size: 5087
DraIII CAC_nnn'GTG
Cuts at: 929 929
Size: 5087
DrdI GACnn_nn'nnGTC
Cuts at: 2793 3651 2793
Size: 858 4229
EaeI y'GGCC_r
Cuts at: 2672 2846 3237 3264 4609 2672
Size: 174 391 27 1345 3150
Fragments arranged by size:
3150 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2672 2672
Size: 5087
EarI CTCTTCn'nnn_
Cuts at: 3110 3320 3110
Size: 210 4877
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3295 3605 3751 4897 3295
Size: 310 146 1146 3485
Fragments arranged by size:
3485 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1793 2099 1793
Size: 306 4781
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 220 231 325 437 598 1011 1029 1179
Size: 61 11 94 112 161 413 18 150
Cuts at: 1179 1347 1431 1683 1767 1851 2099 2912 3344
Size: 168 84 252 84 84 248 813 432
Cuts at: 3344 4091 4359 159
Size: 747 268 887
Fragments arranged by size:
887 813 747 432 413 268 252 248
168 161 150 112 94 84 84 84
61 18 11
EcoO109I rG'GnC_Cy
Cuts at: 215 938 1037 1313 2226 215
Size: 723 99 276 913 3076
Fragments arranged by size:
3076 913 723 276 99
EcoRI G'AATT_C
Cuts at: 2091 2091
Size: 5087
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3106 3106
Size: 5087
FspI TGC'GCA
Cuts at: 2868 2868
Size: 5087
HaeII r_GCGC'y
Cuts at: 2521 2769 3791 4770 2521
Size: 248 1022 979 2838
Fragments arranged by size:
2838 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 368 400 1374 1406 2929 2959 2961 2991 368
Size: 32 974 32 1523 30 2 30 2464
Fragments arranged by size:
2464 1523 974 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2126 2126
Size: 5087
KasI G'GCGC_C
Cuts at: 2517 2765 2517
Size: 248 4839
KpnI G_GTAC'C
Cuts at: 2107 2107
Size: 5087
MluI A'CGCG_T
Cuts at: 4520 4520
Size: 5087
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 452 3758 3942 452
Size: 3306 184 1597
Fragments arranged by size:
3306 1597 184
MscI TGG'CCA
Cuts at: 2848 2848
Size: 5087
MslI CAynn'nnrTG
Cuts at: 559 986 1265 2010 3076 3203 559
Size: 427 279 745 1066 127 2443
Fragments arranged by size:
2443 1066 745 427 279 127
MspA1I CmG'CkG
Cuts at: 198 2198 2512 2872 3885 4130 198
Size: 2000 314 360 1013 245 1155
Fragments arranged by size:
2000 1155 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3268 3268
Size: 5087
NarI GG'CG_CC
Cuts at: 2518 2766 2518
Size: 248 4839
NdeI CA'TA_TG
Cuts at: 2007 2007
Size: 5087
NgoMIV G'CCGG_C
Cuts at: 3266 3266
Size: 5087
NsiI A_TGCA'T
Cuts at: 2013 2013
Size: 5087
NspI r_CATG'y
Cuts at: 2015 2140 2640 3547 2015
Size: 125 500 907 3555
Fragments arranged by size:
3555 907 500 125
PasI CC'CwG_GG
Cuts at: 115 115
Size: 5087
PciI A'CATG_T
Cuts at: 3543 3543
Size: 5087
PmeI GTTT'AAAC
Cuts at: 2086 2086
Size: 5087
PmlI CAC'GTG
Cuts at: 685 685
Size: 5087
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4226 4258 4226
Size: 32 5055
PpuMI rG'GwC_Cy
Cuts at: 215 938 1037 1313 215
Size: 723 99 276 3989
Fragments arranged by size:
3989 723 276 99
PstI C_TGCA'G
Cuts at: 481 2134 2819 481
Size: 1653 685 2749
Fragments arranged by size:
2749 1653 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5087
PvuII CAG'CTG
Cuts at: 198 2512 2872 198
Size: 2314 360 2413
Fragments arranged by size:
2413 2314 360
RsrII CG'GwC_CG
Cuts at: 3282 3282
Size: 5087
SacI G_AGCT'C
Cuts at: 1795 2101 1795
Size: 306 4781
SalI G'TCGA_C
Cuts at: 2124 2124
Size: 5087
SapI GCTCTTCn'nnn_
Cuts at: 3110 3320 3110
Size: 210 4877
SbfI CC_TGCA'GG
Cuts at: 2134 2134
Size: 5087
ScaI AGT'ACT
Cuts at: 4604 4604
Size: 5087
SfcI C'TryA_G
Cuts at: 33 477 2130 2815 3808 3999 33
Size: 444 1653 685 993 191 1121
Fragments arranged by size:
1653 1121 993 685 444 191
SfoI GGC'GCC
Cuts at: 2519 2767 2519
Size: 248 4839
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5087
SmaI CCC'GGG
Cuts at: 2109 2109
Size: 5087
SmlI C'TyrA_G
Cuts at: 2242 3157 3649 3911 2242
Size: 915 492 262 3418
Fragments arranged by size:
3418 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5087
SphI G_CATG'C
Cuts at: 2015 2140 2640 2015
Size: 125 500 4462
Fragments arranged by size:
4462 500 125
StuI AGG'CCT
Cuts at: 772 1192 1528 2032 772
Size: 420 336 504 3827
Fragments arranged by size:
3827 504 420 336
StyI C'CwwG_G
Cuts at: 348 1826 2221 2575 348
Size: 1478 395 354 2860
Fragments arranged by size:
2860 1478 395 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1147 1297 1399 1567 1832 1978 2670 2803 3262
Size: 150 102 168 265 146 692 133 459
Cuts at: 3262 3471 1147
Size: 209 2763
Fragments arranged by size:
2763 692 459 265 209 168 150 146
133 102
TatI w'GTAC_w
Cuts at: 4602 4602
Size: 5087
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 454 486 1192 1224 1612 1644 2032 2064 4604
Size: 32 706 32 388 32 388 32 2540
Cuts at: 4604 4636 454
Size: 32 905
Fragments arranged by size:
2540 905 706 388 388 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2884 2884
Size: 5087
XbaI T'CTAG_A
Cuts at: 337 525 2118 337
Size: 188 1593 3306
Fragments arranged by size:
3306 1593 188
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2582 2582
Size: 5087
XmaI C'CCGG_G
Cuts at: 2107 2107
Size: 5087
XmnI GAAnn'nnTTC
Cuts at: 1024 1108 1360 1444 1528 1864 1024
Size: 84 252 84 84 336 4247
Fragments arranged by size:
4247 336 252 84 84 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI
PasI PciI PmeI PmlI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmtI Bpu10I BsiWI
BsrGI BssHII BstZ17I Bsu36I BtgI ClaI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NheI NotI
NruI PacI PflMI PfoI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI