Restriction map for pF1KB8383
##################################
# NO CUT ENZYMES (  159 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspI        AvaI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BcnI        BfaI        BfrI        
BglI        BlnI        BlpI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmBI       BsmFI       BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRV       EcoT14I     
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MroI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    StuI        StyI        SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AciI          824  GCGG                 CCGC(-3/-1)
AflIII        260  ACGTGT               A^C[GA][CT]GT
AhdI         1665  GACCTTTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         380  CAGACCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          595  ATTAAT               AT^TAAT
AsnI          595  ATTAAT               AT^TAAT
AspEI        1665  GACCTTTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII          75  GGACC                G^G[AT]CC
BalI          630  TGGCCA               TGG^CCA
BbsI         1662  GAAGAC               GAAGAC(2/6)
BclI          576  TGATCA               T^GATCA
BglII         700  AGATCT               A^GATCT
BpuAI        1662  GAAGAC               GAAGAC(2/6)
BsmI          588  GAATGC               GAATGC(1/-1)
Bsp1407I     1524  TGTACA               T^GTACA
BsrDI        1317  GCAATG               GCAATG(2/0)
BsrGI        1524  TGTACA               T^GTACA
BstAPI        815  GCAGTATTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr13I         75  GGACC                G^G[ACGT]CC
DraI          762  TTTAAA               TTT^AAA
EaeI          630  TGGCCA               [CT]^GGCC[GA]
Eam1105I     1665  GACCTTTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        1703  GAATTC               G^AATTC
FauI          824  GCGGG                CCCGC(4/6)
FbaI          576  TGATCA               T^GATCA
HaeIII        631  GGCC                 GG^CC
MaeII         260  ACGT                 A^CGT
MluNI         630  TGGCCA               TGG^CCA
MscI          630  TGGCCA               TGG^CCA
MwoI          815  GCAGTATTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI         622  CCAAGTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI         595  ATTAAT               AT^TAAT
SapI          190  GAAGAGC              GCTCTTC(1/4)
Sau96I         75  GGACC                G^G[ACGT]CC
SspBI        1524  TGTACA               T^GTACA
SspI          445  AATATT               AAT^ATT
TaqI          179  TCGA                 T^CGA
TthHB8I       179  TCGA                 T^CGA
Van91I        622  CCAAGTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          235  CCAGAAAGGAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwI          108  GATCC                GGATC(4/5)
              609  GGATC               
BbvI          272  GCAGC                GCAGC(8/12)
              679  GCAGC               
BsmAI         715  GAGAC                GTCTC(1/5)
              133  GTCTC               
BsrI         1543  ACTGG                ACTGG(1/-1)
              225  CCAGT               
BstYI         609  GGATCT               [GA]^GATC[CT]
              700  AGATCT              
EarI           58  GAAGAG               CTCTTC(1/4)
              190  GAAGAG              
EcoT22I      1400  ATGCAT               ATGCA^T
             1652  ATGCAT              
Fnu4HI        272  GCAGC                GC^[ACGT]GC
              679  GCAGC               
ItaI          272  GCAGC                GC^[ACGT]GC
              679  GCAGC               
Ksp632I        58  GAAGAG               CTCTTC(1/4)
              190  GAAGAG              
MflI          609  GGATCT               [GA]^GATC[CT]
              700  AGATCT              
MslI         1556  CATATAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1710  CACAAGGGTG          
NlaIV          75  GGACCC               GG[ACGT]^[ACGT]CC
              801  GGAGCC              
NsiI         1400  ATGCAT               ATGCA^T
             1652  ATGCAT              
NspI         1402  GCATGT               [GA]CATG^[CT]
             1654  GCATGT              
PleI          421  GACTC                GAGTC(4/5)
             1620  GACTC               
SacI          866  GAGCTC               GAGCT^C
             1115  GAGCTC              
SfaNI         812  GATGC                GCATC(5/9)
             1443  GCATC               
TseI          272  GCAGC                G^C[AT]GC
              679  GCAGC               
XhoII         609  GGATCT               [GA]^GATC[CT]
              700  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        Asp700I     
AspHI       BanII       BmyI        BsaJI       BsiHKAI     
BsiYI       BslI        Bsp1286I    BssKI       BstF5I      
BstNI       DdeI        DpnII       EcoRII      FokI        
HinfI       HphI        MaeIII      MboI        MboII       
MnlI        MseI        MvaI        NdeII       NlaIII      
RsaI        Sau3AI      ScrFI       TfiI        Tru9I       
Tsp45I      Tsp509I     TspRI       XmnI        
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