Restriction map for pF1KB8377
 (Linear) (Six-base) MAPSORT of: KIBB8377.seq  Check: 6669  from: 1  to: 1866



KIBB8377.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     229    1046    1376    1421    1667    1866 
   Size:        229     817     330      45     246     199

  Fragments arranged by size: 

                 817     330     246     229     199      45
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1357    1648    1693    1866 
   Size:       1357     291      45     173

  Fragments arranged by size: 

                1357     291     173      45
 
AleI CACnn'nnGTG

Cuts at:      0      19    1710    1866 
   Size:         19    1691     156

  Fragments arranged by size: 

                1691     156      19
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1074    1866 
   Size:       1074     792
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1126    1158    1866 
   Size:       1126      32     708

  Fragments arranged by size: 

                1126     708      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     657     876    1671    1866 
   Size:        657     219     795     195

  Fragments arranged by size: 

                 795     657     219     195
 
ApaI G_GGCC'C

Cuts at:      0     335    1172    1866 
   Size:        335     837     694

  Fragments arranged by size: 

                 837     694     335
 
ApoI r'AATT_y

Cuts at:      0    1423    1831    1866 
   Size:       1423     408      35

  Fragments arranged by size: 

                1423     408      35
 
AvaI C'yCGr_G

Cuts at:      0    1438    1866 
   Size:       1438     428
 
BamHI G'GATC_C

Cuts at:      0    1796    1866 
   Size:       1796      70
 
BanI G'GyrC_C

Cuts at:      0    1058    1399    1433    1866 
   Size:       1058     341      34     433

  Fragments arranged by size: 

                1058     433     341      34
 
BanII G_rGCy'C

Cuts at:      0      28     149     158     179     335     353    1172    1230 
   Size:         28     121       9      21     156      18     819      58

Cuts at:   1230    1866 
   Size:        636

  Fragments arranged by size: 

                 819     636     156     121      58      28      21      18
                   9
 
BbvCI CC'TCA_GC

Cuts at:      0     563    1866 
   Size:        563    1303
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1419    1453    1516    1550    1866 
   Size:       1419      34      63      34     316

  Fragments arranged by size: 

                1419     316      63      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1581    1866 
   Size:       1581     285
 
BclI T'GATC_A

Cuts at:      0     659    1866 
   Size:        659    1207
 
BglI GCCn_nnn'nGGC

Cuts at:      0      85     428    1866 
   Size:         85     343    1438

  Fragments arranged by size: 

                1438     343      85
 
BlpI GC'TnA_GC

Cuts at:      0    1261    1866 
   Size:       1261     605
 
Bme1580I G_kGCm'C

Cuts at:      0     335    1172    1404    1633    1866 
   Size:        335     837     232     229     233

  Fragments arranged by size: 

                 837     335     233     232     229
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     763     886    1405    1866 
   Size:        763     123     519     461

  Fragments arranged by size: 

                 763     519     461     123
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     175    1866 
   Size:        175    1691
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      12     331     634     871     919     928    1148    1741 
   Size:         12     319     303     237      48       9     220     593

Cuts at:   1741    1866 
   Size:        125

  Fragments arranged by size: 

                 593     319     303     237     220     125      48      12
                   9
 
Bpu10I CC'TnA_GC

Cuts at:      0     128     352     563    1866 
   Size:        128     224     211    1303

  Fragments arranged by size: 

                1303     224     211     128
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     484     652    1359    1766    1866 
   Size:        484     168     707     407     100

  Fragments arranged by size: 

                 707     484     407     168     100
 
BsaHI Gr'CG_yC

Cuts at:      0    1817    1866 
   Size:       1817      49
 
BsaWI w'CCGG_w

Cuts at:      0      97    1866 
   Size:         97    1769
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     391     421    1126    1156    1866 
   Size:        391      30     705      30     710

  Fragments arranged by size: 

                 710     705     391      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     148    1267    1603    1866 
   Size:        148    1119     336     263

  Fragments arranged by size: 

                1119     336     263     148
 
BseYI C'CCAG_C

Cuts at:      0     558    1866 
   Size:        558    1308
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400    1642    1688    1866 
   Size:        400    1242      46     178

  Fragments arranged by size: 

                1242     400     178      46
 
BsiHKAI G_wGCw'C

Cuts at:      0     795    1866 
   Size:        795    1071
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1076    1866 
   Size:       1076     790
 
Bsp1286I G_dGCh'C

Cuts at:      0      28     149     158     179     335     353     795    1172 
   Size:         28     121       9      21     156      18     442     377

Cuts at:   1172    1230    1404    1633    1866 
   Size:         58     174     229     233

  Fragments arranged by size: 

                 442     377     233     229     174     156     121      58
                  28      21      18       9
 
BspHI T'CATG_A

Cuts at:      0     696    1866 
   Size:        696    1170
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     229    1046    1115    1376    1421    1667    1866 
   Size:        229     817      69     261      45     246     199

  Fragments arranged by size: 

                 817     261     246     229     199      69      45
 
BsrFI r'CCGG_y

Cuts at:      0      62    1866 
   Size:         62    1804
 
BsrGI T'GTAC_A

Cuts at:      0     743    1866 
   Size:        743    1123
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      21      36    1866 
   Size:         21      15    1830

  Fragments arranged by size: 

                1830      21      15
 
BstYI r'GATC_y

Cuts at:      0     525    1796    1866 
   Size:        525    1271      70

  Fragments arranged by size: 

                1271     525      70
 
Bsu36I CC'TnA_GG

Cuts at:      0     238     708    1181    1463    1866 
   Size:        238     470     473     282     403

  Fragments arranged by size: 

                 473     470     403     282     238
 
BtgI C'CryG_G

Cuts at:      0     721    1866 
   Size:        721    1145
 
BtsI GCAGTG_nn'

Cuts at:      0    1522    1700    1866 
   Size:       1522     178     166

  Fragments arranged by size: 

                1522     178     166
 
EaeI y'GGCC_r

Cuts at:      0    1581    1866 
   Size:       1581     285
 
EarI CTCTTCn'nnn_

Cuts at:      0    1453    1669    1866 
   Size:       1453     216     197

  Fragments arranged by size: 

                1453     216     197
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      12     331     634     871     919     928    1148    1357 
   Size:         12     319     303     237      48       9     220     209

Cuts at:   1357    1648    1693    1741    1866 
   Size:        291      45      48     125

  Fragments arranged by size: 

                 319     303     291     237     220     209     125      48
                  48      45      12       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     820    1372    1866 
   Size:        820     552     494

  Fragments arranged by size: 

                 820     552     494
 
EcoO109I rG'GnC_Cy

Cuts at:      0     331     471     758     838    1168    1169    1866 
   Size:        331     140     287      80     330       1     697

  Fragments arranged by size: 

                 697     331     330     287     140      80       1
 
EcoRV GAT'ATC

Cuts at:      0     285    1866 
   Size:        285    1581
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     391     423     765     797     988    1020    1668    1700 
   Size:        391      32     342      32     191      32     648      32

Cuts at:   1700    1866 
   Size:        166

  Fragments arranged by size: 

                 648     391     342     191     166      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0     246    1866 
   Size:        246    1620
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     320    1866 
   Size:        320    1546
 
MslI CAynn'nnrTG

Cuts at:      0      19    1710    1748    1866 
   Size:         19    1691      38     118

  Fragments arranged by size: 

                1691     118      38      19
 
MspA1I CmG'CkG

Cuts at:      0      32     187     291     562    1201    1580    1866 
   Size:         32     155     104     271     639     379     286

  Fragments arranged by size: 

                 639     379     286     271     155     104      32
 
PasI CC'CwG_GG

Cuts at:      0     255     600    1866 
   Size:        255     345    1266

  Fragments arranged by size: 

                1266     345     255
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1410    1866 
   Size:       1410     456
 
PfoI T'CCnGG_A

Cuts at:      0     650    1866 
   Size:        650    1216
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     335     367    1126    1158    1866 
   Size:        335      32     759      32     708

  Fragments arranged by size: 

                 759     708     335      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     471     758     838    1866 
   Size:        471     287      80    1028

  Fragments arranged by size: 

                1028     471     287      80
 
PspOMI G'GGCC_C

Cuts at:      0     331    1168    1866 
   Size:        331     837     698

  Fragments arranged by size: 

                 837     698     331
 
PspXI vC'TCGA_Gb

Cuts at:      0    1438    1866 
   Size:       1438     428
 
PstI C_TGCA'G

Cuts at:      0     186     437     593    1092    1529    1866 
   Size:        186     251     156     499     437     337

  Fragments arranged by size: 

                 499     437     337     251     186     156
 
PvuII CAG'CTG

Cuts at:      0      32     187     562    1866 
   Size:         32     155     375    1304

  Fragments arranged by size: 

                1304     375     155      32
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1453    1866 
   Size:       1453     413
 
SbfI CC_TGCA'GG

Cuts at:      0     437     593    1866 
   Size:        437     156    1273

  Fragments arranged by size: 

                1273     437     156
 
SfcI C'TryA_G

Cuts at:      0     182     433     589    1063    1088    1525    1866 
   Size:        182     251     156     474      25     437     341

  Fragments arranged by size: 

                 474     437     341     251     182     156      25
 
SmlI C'TyrA_G

Cuts at:      0    1438    1866 
   Size:       1438     428
 
StuI AGG'CCT

Cuts at:      0     713     856     925    1384    1866 
   Size:        713     143      69     459     482

  Fragments arranged by size: 

                 713     482     459     143      69
 
StyI C'CwwG_G

Cuts at:      0      80     203     642    1138    1325    1866 
   Size:         80     123     439     496     187     541

  Fragments arranged by size: 

                 541     496     439     187     123      80
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1378    1387    1866 
   Size:       1378       9     479

  Fragments arranged by size: 

                1378     479       9
 
TatI w'GTAC_w

Cuts at:      0     743     800    1866 
   Size:        743      57    1066

  Fragments arranged by size: 

                1066     743      57
 
Tth111I GACn'n_nGTC

Cuts at:      0     370    1866 
   Size:        370    1496
 
XhoI C'TCGA_G

Cuts at:      0    1438    1866 
   Size:       1438     428
 
XmnI GAAnn'nnTTC

Cuts at:      0    1832    1866 
   Size:       1832      34
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI
     AvaI    BamHI     BanI    BanII    BbvCI     BcgI    BciVI     BclI
     BglI     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I     BsaI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrFI    BsrGI    BstXI    BstYI   Bsu36I
     BtgI     BtsI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRV
    Hin4I  HindIII     MmeI     MslI   MspA1I     PasI    PflMI     PfoI
     PpiI    PpuMI   PspOMI    PspXI     PstI    PvuII     SapI     SbfI
     SfcI     SmlI     StuI     StyI    TaqII     TatI  Tth111I     XhoI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
     BbsI    BfrBI    BglII    BmgBI     BmtI    BpuEI    BsaAI    BsaBI
    BsiEI    BsiWI     BsmI    BspEI    BsrBI    BsrDI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRI     FalI     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI
    PshAI     PsiI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TstI     XbaI
     XcmI     XmaI     ZraI
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