Restriction map for pF1KB8372
 (Linear) (Six-base) MAPSORT of: KIBB8372.seq  Check: 5905  from: 1  to: 495



KIBB8372.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AccI GT'mk_AC

Cuts at:      0     146     495 
   Size:        146     349
 
AflII C'TTAA_G

Cuts at:      0     417     495 
   Size:        417      78
 
ApoI r'AATT_y

Cuts at:      0      13     495 
   Size:         13     482
 
AseI AT'TA_AT

Cuts at:      0     479     495 
   Size:        479      16
 
AvaI C'yCGr_G

Cuts at:      0     282     495 
   Size:        282     213
 
BamHI G'GATC_C

Cuts at:      0     408     495 
   Size:        408      87
 
BanI G'GyrC_C

Cuts at:      0     343     495 
   Size:        343     152
 
BanII G_rGCy'C

Cuts at:      0     302     495 
   Size:        302     193
 
BbeI G_GCGC'C

Cuts at:      0     347     495 
   Size:        347     148
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      40     495 
   Size:         40     455
 
BlpI GC'TnA_GC

Cuts at:      0      79     495 
   Size:         79     416
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      33     495 
   Size:         33     462
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44      88     495 
   Size:         44      44     407

  Fragments arranged by size: 

                 407      44      44
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     307     495 
   Size:        307     188
 
BsaHI Gr'CG_yC

Cuts at:      0     344     495 
   Size:        344     151
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     495 
   Size:        349     146
 
BsiHKAI G_wGCw'C

Cuts at:      0      80     302     495 
   Size:         80     222     193

  Fragments arranged by size: 

                 222     193      80
 
Bsp1286I G_dGCh'C

Cuts at:      0      80     302     495 
   Size:         80     222     193

  Fragments arranged by size: 

                 222     193      80
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     371     495 
   Size:        371     124
 
BsrFI r'CCGG_y

Cuts at:      0     287     495 
   Size:        287     208
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     372     495 
   Size:        372     123
 
BstYI r'GATC_y

Cuts at:      0     215     408     495 
   Size:        215     193      87

  Fragments arranged by size: 

                 215     193      87
 
BtgI C'CryG_G

Cuts at:      0     329     495 
   Size:        329     166
 
BtsI GCAGTG_nn'

Cuts at:      0     366     495 
   Size:        366     129
 
DraIII CAC_nnn'GTG

Cuts at:      0     372     495 
   Size:        372     123
 
EaeI y'GGCC_r

Cuts at:      0     128     495 
   Size:        128     367
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     196     310     495 
   Size:        196     114     185

  Fragments arranged by size: 

                 196     185     114
 
EcoICRI GAG'CTC

Cuts at:      0     300     495 
   Size:        300     195
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44      88     495 
   Size:         44      44     407

  Fragments arranged by size: 

                 407      44      44
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     232     495 
   Size:        232     263
 
EcoRI G'AATT_C

Cuts at:      0      13     495 
   Size:         13     482
 
HaeII r_GCGC'y

Cuts at:      0     347     495 
   Size:        347     148
 
HincII GTy'rAC

Cuts at:      0     147     424     495 
   Size:        147     277      71

  Fragments arranged by size: 

                 277     147      71
 
KasI G'GCGC_C

Cuts at:      0     343     495 
   Size:        343     152
 
MslI CAynn'nnrTG

Cuts at:      0     399     495 
   Size:        399      96
 
MspA1I CmG'CkG

Cuts at:      0     331     495 
   Size:        331     164
 
NaeI GCC'GGC

Cuts at:      0     289     495 
   Size:        289     206
 
NarI GG'CG_CC

Cuts at:      0     344     495 
   Size:        344     151
 
NgoMIV G'CCGG_C

Cuts at:      0     287     495 
   Size:        287     208
 
PstI C_TGCA'G

Cuts at:      0     215     495 
   Size:        215     280
 
SacI G_AGCT'C

Cuts at:      0     302     495 
   Size:        302     193
 
SacII CC_GC'GG

Cuts at:      0     332     495 
   Size:        332     163
 
SalI G'TCGA_C

Cuts at:      0     145     495 
   Size:        145     350
 
SfcI C'TryA_G

Cuts at:      0     211     495 
   Size:        211     284
 
SfoI GGC'GCC

Cuts at:      0     345     495 
   Size:        345     150
 
SmaI CCC'GGG

Cuts at:      0     284     495 
   Size:        284     211
 
SmlI C'TyrA_G

Cuts at:      0     417     495 
   Size:        417      78
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      52     331     495 
   Size:         52     279     164

  Fragments arranged by size: 

                 279     164      52
 
XmaI C'CCGG_G

Cuts at:      0     282     495 
   Size:        282     213
 

 Enzymes that do cut:

     AccI    AflII     ApoI     AseI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI     BlpI     BplI     BpmI     BsaI    BsaHI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrFI   BstAPI    BstYI     BtgI     BtsI
   DraIII     EaeI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    HaeII
   HincII     KasI     MslI   MspA1I     NaeI     NarI   NgoMIV     PstI
     SacI    SacII     SalI     SfcI     SfoI     SmaI     SmlI    TaqII
     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI    AvrII     BaeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI     DrdI     EagI     EarI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI
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