Restriction map for pF1KB8369
 (Linear) (Six-base) MAPSORT of: KIBB8369.seq  Check: 5867  from: 1  to: 594



KIBB8369.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AatII G_ACGT'C

Cuts at:      0     588     594 
   Size:        588       6
 
ApoI r'AATT_y

Cuts at:      0     180     594 
   Size:        180     414
 
AvaI C'yCGr_G

Cuts at:      0     125     253     594 
   Size:        125     128     341

  Fragments arranged by size: 

                 341     128     125
 
BanI G'GyrC_C

Cuts at:      0     289     300     345     594 
   Size:        289      11      45     249

  Fragments arranged by size: 

                 289     249      45      11
 
BanII G_rGCy'C

Cuts at:      0      31     363     594 
   Size:         31     332     231

  Fragments arranged by size: 

                 332     231      31
 
BbeI G_GCGC'C

Cuts at:      0     349     594 
   Size:        349     245
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     588     594 
   Size:        588       6
 
BglI GCCn_nnn'nGGC

Cuts at:      0      58     323     594 
   Size:         58     265     271

  Fragments arranged by size: 

                 271     265      58
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     594 
   Size:         58     536
 
BsaHI Gr'CG_yC

Cuts at:      0      50     346     559     585     594 
   Size:         50     296     213      26       9

  Fragments arranged by size: 

                 296     213      50      26       9
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     594 
   Size:         68     526
 
Bsp1286I G_dGCh'C

Cuts at:      0      31      68     363     594 
   Size:         31      37     295     231

  Fragments arranged by size: 

                 295     231      37      31
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     471     594 
   Size:        471     123
 
BsrBI CCG'CTC

Cuts at:      0      42     594 
   Size:         42     552
 
BsrDI GCAATG_nn'

Cuts at:      0     445     594 
   Size:        445     149
 
BsrFI r'CCGG_y

Cuts at:      0     303     541     594 
   Size:        303     238      53

  Fragments arranged by size: 

                 303     238      53
 
BtgI C'CryG_G

Cuts at:      0     274     594 
   Size:        274     320
 
BtsI GCAGTG_nn'

Cuts at:      0     141     594 
   Size:        141     453
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     408     594 
   Size:        408     186
 
EarI CTCTTCn'nnn_

Cuts at:      0     100     110     152     594 
   Size:        100      10      42     442

  Fragments arranged by size: 

                 442     100      42      10
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      76     594 
   Size:         76     518
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     594 
   Size:         58     536
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     264     594 
   Size:        264     330
 
FspI TGC'GCA

Cuts at:      0     444     594 
   Size:        444     150
 
HaeII r_GCGC'y

Cuts at:      0     349     594 
   Size:        349     245
 
HincII GTy'rAC

Cuts at:      0     122     594 
   Size:        122     472
 
HpaI GTT'AAC

Cuts at:      0     122     594 
   Size:        122     472
 
KasI G'GCGC_C

Cuts at:      0     345     594 
   Size:        345     249
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     392     594 
   Size:        392     202
 
MspA1I CmG'CkG

Cuts at:      0     276     329     594 
   Size:        276      53     265

  Fragments arranged by size: 

                 276     265      53
 
NaeI GCC'GGC

Cuts at:      0     305     594 
   Size:        305     289
 
NarI GG'CG_CC

Cuts at:      0     346     594 
   Size:        346     248
 
NgoMIV G'CCGG_C

Cuts at:      0     303     594 
   Size:        303     291
 
PstI C_TGCA'G

Cuts at:      0      88     148     594 
   Size:         88      60     446

  Fragments arranged by size: 

                 446      88      60
 
SacII CC_GC'GG

Cuts at:      0     277     594 
   Size:        277     317
 
SbfI CC_TGCA'GG

Cuts at:      0      88     594 
   Size:         88     506
 
SfcI C'TryA_G

Cuts at:      0      84     144     264     594 
   Size:         84      60     120     330

  Fragments arranged by size: 

                 330     120      84      60
 
SfoI GGC'GCC

Cuts at:      0     347     594 
   Size:        347     247
 
SmaI CCC'GGG

Cuts at:      0     127     594 
   Size:        127     467
 
XmaI C'CCGG_G

Cuts at:      0     125     594 
   Size:        125     469
 
XmnI GAAnn'nnTTC

Cuts at:      0      94     594 
   Size:         94     500
 
ZraI GAC'GTC

Cuts at:      0     586     594 
   Size:        586       8
 

 Enzymes that do cut:

    AatII     ApoI     AvaI     BanI    BanII     BbeI     BbsI     BglI
     BpmI    BsaHI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
     BtgI     BtsI     DrdI     EarI     EciI  Eco57MI     FalI     FspI
    HaeII   HincII     HpaI     KasI     MmeI   MspA1I     NaeI     NarI
   NgoMIV     PstI    SacII     SbfI     SfcI     SfoI     SmaI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     EaeI     EagI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI    FspAI    Hin4I  HindIII     KpnI     MfeI     MluI     MscI
     MslI     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI
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