Restriction map for pF1KB8368
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AhdI        
Aor51HI     AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BbeI        BbrPI       BbvCI       BcgI        BclI        
BfrI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaHI       BseAI       BseRI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstZ17I     
CelII       CfoI        ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoRI       EcoRV       FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HpaI        KasI        KpnI        KspI        
MfeI        MluI        MroI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        SspBI       SwaI        
Tth111I     XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AcsI          506  AAATTC               [GA]^AATT[CT]
AfaI          273  GTAC                 GT^AC
AgeI          238  ACCGGT               A^CCGGT
Alw44I       1334  GTGCAC               G^TGCAC
AlwNI         809  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1129  CCTGAGG              CC^T[ACGT]AGG
ApaI         1323  GGGCCC               GGGCC^C
ApaLI        1334  GTGCAC               G^TGCAC
ApoI          506  AAATTC               [GA]^AATT[CT]
AspHI        1334  GTGCAC               G[AT]GC[AT]^C
BalI         1775  TGGCCA               TGG^CCA
BanI         1680  GGTGCC               G^G[CT][GA]CC
BanII        1323  GGGCCC               G[GA]GC[CT]^C
BbsI          421  GAAGAC               GAAGAC(2/6)
BglII          95  AGATCT               A^GATCT
BpuAI         421  GAAGAC               GAAGAC(2/6)
BsaBI         838  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          452  GGTCTC               GGTCTC(1/5)
BsaWI         238  ACCGGT               [AT]^CCGG[AT]
BsgI         1803  GTGCAG               GTGCAG(16/14)
BsiHKAI      1334  GTGCAC               G[AT]GC[AT]^C
BspHI         299  TCATGA               T^CATGA
BsrDI        1565  CATTGC               GCAATG(2/0)
BstEII        789  GGTGACC              G^GT[ACGT]ACC
BstPI         789  GGTGACC              G^GT[ACGT]ACC
Bsu36I       1129  CCTGAGG              CC^T[ACGT]AGG
DsaI          633  CCATGG               C^C[GA][CT]GG
EarI         1405  GAAGAG               CTCTTC(1/4)
Eco81I       1129  CCTGAGG              CC^T[ACGT]AGG
EcoNI         606  CCTTGTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       789  GGTGACC              G^GT[ACGT]ACC
EcoT22I       214  ATGCAT               ATGCA^T
FauI         1840  CCCGC                CCCGC(4/6)
HincII       1543  GTCAAC               GT[CT]^[GA]AC
HindII       1543  GTCAAC               GT[CT]^[GA]AC
HindIII      1440  AAGCTT               A^AGCTT
Ksp632I      1405  GAAGAG               CTCTTC(1/4)
MamI          838  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1775  TGGCCA               TGG^CCA
MscI         1775  TGGCCA               TGG^CCA
NcoI          633  CCATGG               C^CATGG
NsiI          214  ATGCAT               ATGCA^T
PinAI         238  ACCGGT               A^CCGGT
PpuMI        1175  GGGACCC              [GA]G^G[AT]CC[CT]
PstI         1328  CTGCAG               CTGCA^G
RcaI          299  TCATGA               T^CATGA
RsaI          273  GTAC                 GT^AC
SbfI         1327  CCTGCAGG             CCTGCA^GG
SmaI         1609  CCCGGG               CCC^GGG
Sse8387I     1327  CCTGCAGG             CCTGCA^GG
SspI         1294  AATATT               AAT^ATT
StuI          145  AGGCCT               AGG^CCT
TaqI          510  TCGA                 T^CGA
TthHB8I       510  TCGA                 T^CGA
XbaI         1399  TCTAGA               T^CTAGA
XcmI          314  CCACTAACCAGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1609  CCCGGG               C^CCGGG
XmaI         1609  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AvaI          520  CCCGAG               C^[CT]CG[GA]G
             1609  CCCGGG              
AvaII         963  GGACC                G^G[AT]CC
             1176  GGACC               
BciVI         637  GGATAC               GTATCC(6/5)
             1464  GGATAC              
BfaI          680  CTAG                 C^TAG
             1400  CTAG                
BglI          257  GCCACTTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1726  GCCAAAGAGGC         
BmyI         1323  GGGCCC               G[AGT]GC[ACT]^C
             1334  GTGCAC              
BpmI         1810  CTCCAG               CTGGAG(16/14)
              642  CTGGAG              
BsoBI         520  CCCGAG               C^[CT]CG[GA]G
             1609  CCCGGG              
Bsp1286I     1323  GGGCCC               G[AGT]GC[ACT]^C
             1334  GTGCAC              
BsrFI         238  ACCGGT               [GA]^CCGG[CT]
             1338  ACCGGC              
Cfr10I        238  ACCGGT               [GA]^CCGG[CT]
             1338  ACCGGC              
DraI          100  TTTAAA               TTT^AAA
             1374  TTTAAA              
EaeI         1340  CGGCCA               [CT]^GGCC[GA]
             1775  TGGCCA              
MaeI          680  CTAG                 C^TAG
             1400  CTAG                
MaeII         359  ACGT                 A^CGT
             1703  ACGT                
NspI         1692  ACATGC               [GA]CATG^[CT]
             1843  GCATGT              
PflMI         628  CCAGACCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              808  CCAGTTCCTGG         
PvuII         530  CAGCTG               CAG^CTG
             1813  CAGCTG              
SfcI          780  CTATAG               C^T[GA][CT]AG
             1328  CTGCAG              
Tsp509I       175  AATT                 ^AATT
              507  AATT                
Van91I        628  CCAGACCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              808  CCAGTTCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AciI        AluI        AlwI        BamHI       BbvI        
BcnI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       BspMI       BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TfiI        Tru9I       TseI        Tsp45I      TspRI       
XhoII       
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