Restriction map for pF1KB8368
 (Linear) (Six-base) MAPSORT of: KIBB8368.seq  Check: 308  from: 1  to: 1866



KIBB8368.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      64     142    1321    1866 
   Size:         64      78    1179     545

  Fragments arranged by size: 

                1179     545      78      64
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     887    1866 
   Size:        887     979
 
AgeI A'CCGG_T

Cuts at:      0     238    1866 
   Size:        238    1628
 
AlwNI CAG_nnn'CTG

Cuts at:      0     814    1866 
   Size:        814    1052
 
ApaI G_GGCC'C

Cuts at:      0    1327    1866 
   Size:       1327     539
 
ApaLI G'TGCA_C

Cuts at:      0    1334    1866 
   Size:       1334     532
 
ApoI r'AATT_y

Cuts at:      0     506    1866 
   Size:        506    1360
 
AvaI C'yCGr_G

Cuts at:      0     520    1609    1866 
   Size:        520    1089     257

  Fragments arranged by size: 

                1089     520     257
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     630     663     692     725    1866 
   Size:        630      33      29      33    1141

  Fragments arranged by size: 

                1141     630      33      33      29
 
BamHI G'GATC_C

Cuts at:      0    1029    1228    1836    1866 
   Size:       1029     199     608      30

  Fragments arranged by size: 

                1029     608     199      30
 
BanI G'GyrC_C

Cuts at:      0    1680    1866 
   Size:       1680     186
 
BanII G_rGCy'C

Cuts at:      0    1327    1866 
   Size:       1327     539
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     428    1866 
   Size:        428    1438
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     631    1458    1866 
   Size:        631     827     408

  Fragments arranged by size: 

                 827     631     408
 
BfrBI ATG'CAT

Cuts at:      0     216    1866 
   Size:        216    1650
 
BglI GCCn_nnn'nGGC

Cuts at:      0     263    1732    1866 
   Size:        263    1469     134

  Fragments arranged by size: 

                1469     263     134
 
BglII A'GATC_T

Cuts at:      0      95    1866 
   Size:         95    1771
 
Bme1580I G_kGCm'C

Cuts at:      0    1327    1338    1866 
   Size:       1327      11     528

  Fragments arranged by size: 

                1327     528      11
 
BmgBI CAC'GTC

Cuts at:      0     360    1866 
   Size:        360    1506
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1176    1866 
   Size:       1176     690
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     226    1866 
   Size:        226    1640
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     663    1795    1866 
   Size:        663    1132      71

  Fragments arranged by size: 

                1132     663      71
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     458    1866 
   Size:        458    1408
 
BsaBI GATnn'nnATC

Cuts at:      0     842    1866 
   Size:        842    1024
 
BsaWI w'CCGG_w

Cuts at:      0     238    1866 
   Size:        238    1628
 
BseYI C'CCAG_C

Cuts at:      0     553    1261    1421    1815    1866 
   Size:        553     708     160     394      51

  Fragments arranged by size: 

                 708     553     394     160      51
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1824    1866 
   Size:       1824      42
 
BsiHKAI G_wGCw'C

Cuts at:      0    1338    1866 
   Size:       1338     528
 
Bsp1286I G_dGCh'C

Cuts at:      0    1327    1338    1866 
   Size:       1327      11     528

  Fragments arranged by size: 

                1327     528      11
 
BspHI T'CATG_A

Cuts at:      0     299    1866 
   Size:        299    1567
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      64     142     802    1321    1351    1866 
   Size:         64      78     660     519      30     515

  Fragments arranged by size: 

                 660     519     515      78      64      30
 
BsrDI GCAATG_nn'

Cuts at:      0    1564    1866 
   Size:       1564     302
 
BsrFI r'CCGG_y

Cuts at:      0     238    1338    1866 
   Size:        238    1100     528

  Fragments arranged by size: 

                1100     528     238
 
BstEII G'GTnAC_C

Cuts at:      0     789    1866 
   Size:        789    1077
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     553    1026    1819    1866 
   Size:        553     473     793      47

  Fragments arranged by size: 

                 793     553     473      47
 
BstYI r'GATC_y

Cuts at:      0      95     542     713    1029    1228    1279    1686    1836 
   Size:         95     447     171     316     199      51     407     150

Cuts at:   1836    1866 
   Size:         30

  Fragments arranged by size: 

                 447     407     316     199     171     150      95      51
                  30
 
Bsu36I CC'TnA_GG

Cuts at:      0    1130    1866 
   Size:       1130     736
 
BtgI C'CryG_G

Cuts at:      0     633    1866 
   Size:        633    1233
 
BtsI GCAGTG_nn'

Cuts at:      0     770    1784    1866 
   Size:        770    1014      82

  Fragments arranged by size: 

                1014     770      82
 
DraI TTT'AAA

Cuts at:      0     102    1376    1866 
   Size:        102    1274     490

  Fragments arranged by size: 

                1274     490     102
 
EaeI y'GGCC_r

Cuts at:      0    1340    1775    1866 
   Size:       1340     435      91

  Fragments arranged by size: 

                1340     435      91
 
EarI CTCTTCn'nnn_

Cuts at:      0    1400    1866 
   Size:       1400     466
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     794    1866 
   Size:        794    1072
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     663     887    1795    1866 
   Size:        663     224     908      71

  Fragments arranged by size: 

                 908     663     224      71
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     610    1866 
   Size:        610    1256
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1176    1324    1734    1866 
   Size:       1176     148     410     132

  Fragments arranged by size: 

                1176     410     148     132
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1027    1059    1866 
   Size:       1027      32     807

  Fragments arranged by size: 

                1027     807      32
 
HincII GTy'rAC

Cuts at:      0    1545    1866 
   Size:       1545     321
 
HindIII A'AGCT_T

Cuts at:      0    1440    1866 
   Size:       1440     426
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     830    1866 
   Size:        830    1036
 
MscI TGG'CCA

Cuts at:      0    1777    1866 
   Size:       1777      89
 
MslI CAynn'nnrTG

Cuts at:      0     280     301    1192    1223    1672    1866 
   Size:        280      21     891      31     449     194

  Fragments arranged by size: 

                 891     449     280     194      31      21
 
MspA1I CmG'CkG

Cuts at:      0     532    1592    1815    1866 
   Size:        532    1060     223      51

  Fragments arranged by size: 

                1060     532     223      51
 
NcoI C'CATG_G

Cuts at:      0     633    1866 
   Size:        633    1233
 
NsiI A_TGCA'T

Cuts at:      0     218    1866 
   Size:        218    1648
 
NspI r_CATG'y

Cuts at:      0    1696    1847    1866 
   Size:       1696     151      19

  Fragments arranged by size: 

                1696     151      19
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     634     814    1866 
   Size:        634     180    1052

  Fragments arranged by size: 

                1052     634     180
 
PfoI T'CCnGG_A

Cuts at:      0      81     591    1866 
   Size:         81     510    1275

  Fragments arranged by size: 

                1275     510      81
 
PpuMI rG'GwC_Cy

Cuts at:      0    1176    1866 
   Size:       1176     690
 
PspOMI G'GGCC_C

Cuts at:      0    1323    1866 
   Size:       1323     543
 
PstI C_TGCA'G

Cuts at:      0    1332    1866 
   Size:       1332     534
 
PvuII CAG'CTG

Cuts at:      0     532    1815    1866 
   Size:        532    1283      51

  Fragments arranged by size: 

                1283     532      51
 
SanDI GG'GwC_CC

Cuts at:      0    1176    1866 
   Size:       1176     690
 
SbfI CC_TGCA'GG

Cuts at:      0    1332    1866 
   Size:       1332     534
 
SfcI C'TryA_G

Cuts at:      0     780    1328    1866 
   Size:        780     548     538

  Fragments arranged by size: 

                 780     548     538
 
SmaI CCC'GGG

Cuts at:      0    1611    1866 
   Size:       1611     255
 
SspI AAT'ATT

Cuts at:      0    1296    1866 
   Size:       1296     570
 
StuI AGG'CCT

Cuts at:      0     147    1866 
   Size:        147    1719
 
StyI C'CwwG_G

Cuts at:      0     633     884    1517    1640    1866 
   Size:        633     251     633     123     226

  Fragments arranged by size: 

                 633     633     251     226     123
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1013    1807    1866 
   Size:       1013     794      59

  Fragments arranged by size: 

                1013     794      59
 
TatI w'GTAC_w

Cuts at:      0     272    1866 
   Size:        272    1594
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1168    1200    1866 
   Size:       1168      32     666

  Fragments arranged by size: 

                1168     666      32
 
XbaI T'CTAG_A

Cuts at:      0    1399    1866 
   Size:       1399     467
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     321    1866 
   Size:        321    1545
 
XmaI C'CCGG_G

Cuts at:      0    1609    1866 
   Size:       1609     257
 

 Enzymes that do cut:

     AarI     AcuI     AgeI    AlwNI     ApaI    ApaLI     ApoI     AvaI
     BaeI    BamHI     BanI    BanII     BbsI    BciVI    BfrBI     BglI
    BglII Bme1580I    BmgBI     BmrI     BplI     BpmI     BsaI    BsaBI
    BsaWI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI
    BsrFI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI     DraI
     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I    Hin4I   HincII
  HindIII     MmeI     MscI     MslI   MspA1I     NcoI     NsiI     NspI
    PflMI     PfoI    PpuMI   PspOMI     PstI    PvuII    SanDI     SbfI
     SfcI     SmaI     SspI     StuI     StyI    TaqII     TatI     TstI
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
     AleI     AlfI     AloI     AscI     AseI    AsiSI    AvrII     BbeI
    BbvCI     BcgI     BclI     BlpI     BmtI   Bpu10I    BpuEI    BsaAI
    BsaHI    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I    BtgZI
     ClaI    CspCI   DraIII     DrdI     EagI  EcoICRI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
     SapI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SwaI  Tth111I     XhoI     XmnI
     ZraI
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