Restriction map for pF1KB8363
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AgeI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     AvrII       BalI        BamHI       BbeI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmI        Bsp1407I    BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstUI       BstZ17I     Bsu36I      CelII       Cfr10I      
CpoI        DraI        DraIII      DrdI        EaeI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoRV       FauI        FbaI        FseI        
HaeII       Hin1I       HindIII     HpaI        KasI        
KspI        MaeI        MfeI        MluI        MluNI       
MroI        MscI        MspA1I      MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PinAI       
PmeI        PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
StuI        SwaI        XbaI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

Acc65I       1094  GGTACC               G^GTACC
AciI         1092  GCGG                 CCGC(-3/-1)
AflIII        557  ACGTGT               A^C[GA][CT]GT
AhdI          925  GACACCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         1297  GGGCCC               GGGCC^C
Asp718I      1094  GGTACC               G^GTACC
AspEI         925  GACACCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         415  GAGCTC               G[AT]GC[AT]^C
AspI         1111  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1210  CTCGAG               C^[CT]CG[GA]G
AviII        1121  TGCGCA               TGC^GCA
BanI         1094  GGTACC               G^G[CT][GA]CC
BbrPI        1375  CACGTG               CAC^GTG
BbsI         1380  GAAGAC               GAAGAC(2/6)
BpmI          269  CTGGAG               CTGGAG(16/14)
BpuAI        1380  GAAGAC               GAAGAC(2/6)
BsaAI        1375  CACGTG               [CT]AC^GT[GA]
BsaBI         201  GATGGGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI         1310  GTGCAG               GTGCAG(16/14)
BsiHKAI       415  GAGCTC               G[AT]GC[AT]^C
BsmBI        1365  GAGACG               CGTCTC(1/5)
BsmFI        1171  GGGAC                GGGAC(10/14)
BsoBI        1210  CTCGAG               C^[CT]CG[GA]G
BspDI        1147  ATCGAT               AT^CGAT
BstAPI        212  GCAGTGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         412  CCAGAGCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI         1122  GCGC                 GCG^C
ClaI         1147  ATCGAT               AT^CGAT
Eam1105I      925  GACACCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI         247  GAATTC               G^AATTC
FspI         1121  TGCGCA               TGC^GCA
HhaI         1122  GCGC                 GCG^C
HinP1I       1122  GCGC                 G^CGC
HincII        561  GTTGAC               GT[CT]^[GA]AC
HindII        561  GTTGAC               GT[CT]^[GA]AC
KpnI         1094  GGTACC               GGTAC^C
MamI          201  GATGGGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI         1328  CCATGG               C^CATGG
PaeR7I       1210  CTCGAG               C^TCGAG
PflFI        1111  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         142  CCAGGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1375  CACGTG               CAC^GTG
PmlI         1375  CACGTG               CAC^GTG
PpuMI         144  AGGTCCT              [GA]G^G[AT]CC[CT]
PshAI        1492  GACAGGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          364  CTGCAG               CTGCA^G
SacI          415  GAGCTC               GAGCT^C
SapI          603  GCTCTTC              GCTCTTC(1/4)
SexAI         141  ACCAGGT              A^CC[AT]GGT
SfcI          364  CTGCAG               C^T[GA][CT]AG
SphI         1088  GCATGC               GCATG^C
SspI         1189  AATATT               AAT^ATT
Tth111I      1111  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        142  CCAGGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1008  CCAGACTGTTATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1210  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AvaII         145  GGTCC                G^G[AT]CC
              169  GGACC               
BanII         415  GAGCTC               G[GA]GC[CT]^C
             1297  GGGCCC              
BcnI          234  CCGGG                CC^[GC]GG
              463  CCCGG               
BsiYI         142  CCAGGTCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              195  CCTGTGGATGG         
BslI          142  CCAGGTCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              195  CCTGTGGATGG         
BsrDI         963  CATTGC               GCAATG(2/0)
             1385  CATTGC              
BstEII        531  GGTGACC              G^GT[ACGT]ACC
              865  GGTGACC             
BstNI         142  CCAGG                CC^[AT]GG
              148  CCTGG               
BstPI         531  GGTGACC              G^GT[ACGT]ACC
              865  GGTGACC             
BstYI         263  GGATCT               [GA]^GATC[CT]
              614  GGATCT              
EcoO65I       531  GGTGACC              G^GT[ACGT]ACC
              865  GGTGACC             
EcoRII        142  CCAGG                ^CC[AT]GG
              148  CCTGG               
EcoT14I       651  CCAAGG               C^C[AT][AT]GG
             1328  CCATGG              
EcoT22I       379  ATGCAT               ATGCA^T
              665  ATGCAT              
HapII         234  CCGG                 C^CGG
              464  CCGG                
HgaI          788  GACGC                GACGC(5/10)
              573  GCGTC               
HpaII         234  CCGG                 C^CGG
              464  CCGG                
MflI          263  GGATCT               [GA]^GATC[CT]
              614  GGATCT              
MspI          234  CCGG                 C^CGG
              464  CCGG                
MvaI          142  CCAGG                CC^[AT]GG
              148  CCTGG               
NciI          234  CCGGG                CC^[GC]GG
              463  CCCGG               
NsiI          379  ATGCAT               ATGCA^T
              665  ATGCAT              
NspI         1088  GCATGC               [GA]CATG^[CT]
             1457  ACATGC              
StyI          651  CCAAGG               C^C[AT][AT]GG
             1328  CCATGG              
XhoII         263  GGATCT               [GA]^GATC[CT]
              614  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        AlwNI       
ApoI        BbvI        BmyI        BsaJI       BseRI       
BsmAI       Bsp1286I    BsrI        BssKI       BstF5I      
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EarI        EcoO109I    Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MslI        MwoI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SmlI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     
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