Restriction map for pF1KB8362
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AclI        AcsI        AflII       AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AviII       AvrII       BalI        BbeI        
BbrPI       BbsI        BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsaI        BsiWI       Bsp1407I    
BspDI       BspHI       BspMI       BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    Eco47III    Eco81I      
EcoNI       EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HindIII     HpaI        
KasI        KspI        MamI        MfeI        MluI        
MluNI       MscI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SapI        
SbfI        ScaI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        Tsp509I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

Acc65I        221  GGTACC               G^GTACC
AccIII        691  TCCGGA               T^CCGGA
AflIII       1196  ACATGT               A^C[GA][CT]GT
ApaI          382  GGGCCC               GGGCC^C
Asp718I       221  GGTACC               G^GTACC
AspHI          52  GTGCTC               G[AT]GC[AT]^C
AspI         1003  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         274  GGATCC               G^GATCC
BbvCI          90  CCTCAGC              CCTCAGC(-5/-2)
BfaI         1106  CTAG                 C^TAG
BsaAI         673  TACGTG               [CT]AC^GT[GA]
BsaWI         691  TCCGGA               [AT]^CCGG[AT]
BseAI         691  TCCGGA               T^CCGGA
BsgI          214  GTGCAG               GTGCAG(16/14)
BsiHKAI        52  GTGCTC               G[AT]GC[AT]^C
BsmAI         481  GTCTC                GTCTC(1/5)
BsmBI         480  CGTCTC               CGTCTC(1/5)
BsmI          990  GAATGC               GAATGC(1/-1)
BspEI         691  TCCGGA               T^CCGGA
BspLU11I     1196  ACATGT               A^CATGT
BsrDI        1013  GCAATG               GCAATG(2/0)
BsrI         1143  ACTGG                ACTGG(1/-1)
BstAPI        851  GCAACGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1314  GGTCACC              G^GT[ACGT]ACC
BstPI        1314  GGTCACC              G^GT[ACGT]ACC
BstXI         597  CCACTCCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         274  GGATCC               [GA]^GATC[CT]
DpnII         275  GATC                 ^GATC
EcoO65I      1314  GGTCACC              G^GT[ACGT]ACC
HgaI          109  GACGC                GACGC(5/10)
HincII        639  GTCGAC               GT[CT]^[GA]AC
HindII        639  GTCGAC               GT[CT]^[GA]AC
KpnI          221  GGTACC               GGTAC^C
MaeI         1106  CTAG                 C^TAG
MboI          275  GATC                 ^GATC
MflI          274  GGATCC               [GA]^GATC[CT]
MroI          691  TCCGGA               T^CCGGA
MspA1I        124  CCGCTG               C[AC]G^C[GT]G
NaeI          517  GCCGGC               GCC^GGC
NcoI         1322  CCATGG               C^CATGG
NdeII         275  GATC                 ^GATC
NgoMIV        517  GCCGGC               G^CCGGC
PflFI        1003  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         974  CCACACTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1001  GGGACCC              [GA]G^G[AT]CC[CT]
PshAI        1193  GACACATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          923  CTGCAG               CTGCA^G
SalI          639  GTCGAC               G^TCGAC
Sau3AI        275  GATC                 ^GATC
SexAI         557  ACCAGGT              A^CC[AT]GGT
SfcI          923  CTGCAG               C^T[GA][CT]AG
SfiI          516  GGCCGGCTCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          550  CTCAAG               C^T[CT][GA]AG
SspI         1045  AATATT               AAT^ATT
TfiI          336  GAATC                G^A[AT]TC
Tth111I      1003  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        974  CCACACTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          707  CCAGCAGCCTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         274  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI          394  GTCTAC               GT^[AC][GT]AC
              639  GTCGAC              
AccII          23  CGCG                 CG^CG
              111  CGCG                
AlwI          275  GATCC                GGATC(4/5)
              274  GGATC               
BanI          221  GGTACC               G^G[CT][GA]CC
              561  GGTGCC              
BglI          517  GCCGGCTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              713  GCCTGCTGGGC         
BsiEI         515  CGGCCG               CG[GA][CT]^CG
             1178  CGGCCG              
BstUI          23  CGCG                 CG^CG
              111  CGCG                
CfoI           24  GCGC                 GCG^C
              115  GCGC                
EaeI          515  CGGCCG               [CT]^GGCC[GA]
             1178  CGGCCG              
EagI          515  CGGCCG               C^GGCCG
             1178  CGGCCG              
EarI          448  CTCTTC               CTCTTC(1/4)
              697  CTCTTC              
EclXI         515  CGGCCG               C^GGCCG
             1178  CGGCCG              
Eco52I        515  CGGCCG               C^GGCCG
             1178  CGGCCG              
EcoT14I       758  CCAAGG               C^C[AT][AT]GG
             1322  CCATGG              
HhaI           24  GCGC                 GCG^C
              115  GCGC                
HinP1I         24  GCGC                 G^CGC
              115  GCGC                
Ksp632I       448  CTCTTC               CTCTTC(1/4)
              697  CTCTTC              
MaeIII          7  GTGAC                ^GT[ACGT]AC
             1315  GTCAC               
MvnI           23  CGCG                 CG^CG
              111  CGCG                
NspI         1196  ACATGT               [GA]CATG^[CT]
             1241  ACATGC              
SmaI           84  CCCGGG               CCC^GGG
              378  CCCGGG              
StyI          758  CCAAGG               C^C[AT][AT]GG
             1322  CCATGG              
Tsp45I          7  GTGAC                ^GT[GC]AC
             1315  GTCAC               
TspRI         815  CACTG                CA[GC]TG[ACGT][ACGT]^
             1142  CACTG               
XmaCI          84  CCCGGG               C^CCGGG
              378  CCCGGG              
XmaI           84  CCCGGG               C^CCGGG
              378  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AatII       AciI        AcyI        AfaI        AluI        
AvaI        AvaII       BanII       BbvI        BcnI        
BmyI        BpmI        BsaHI       BsaJI       BseRI       
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BsrFI       BssKI       BstF5I      BstNI       Cac8I       
Cfr10I      Cfr13I      DdeI        DraII       DsaI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       Hin1I       HinfI       HpaII       
HphI        ItaI        MaeII       MboII       MnlI        
MseI        MslI        MspI        MvaI        MwoI        
NciI        NlaIII      NlaIV       PleI        RsaI        
Sau96I      ScrFI       SfaNI       TaqI        Tru9I       
TseI        TthHB8I     
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