Restriction map for pF1KB8358
 (Linear) (Six-base) MAPSORT of: KIBB8358.seq  Check: 6638  from: 1  to: 1032



KIBB8358.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AccI GT'mk_AC

Cuts at:      0     236    1032 
   Size:        236     796
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     625    1032 
   Size:        625     407
 
AleI CACnn'nnGTG

Cuts at:      0      91    1032 
   Size:         91     941
 
ApaI G_GGCC'C

Cuts at:      0     448    1032 
   Size:        448     584
 
ApoI r'AATT_y

Cuts at:      0     406    1032 
   Size:        406     626
 
BanI G'GyrC_C

Cuts at:      0     418     720     739    1032 
   Size:        418     302      19     293

  Fragments arranged by size: 

                 418     302     293      19
 
BanII G_rGCy'C

Cuts at:      0     372     448    1032 
   Size:        372      76     584

  Fragments arranged by size: 

                 584     372      76
 
BfrBI ATG'CAT

Cuts at:      0       3     525    1032 
   Size:          3     522     507

  Fragments arranged by size: 

                 522     507       3
 
BglI GCCn_nnn'nGGC

Cuts at:      0     922    1032 
   Size:        922     110
 
BlpI GC'TnA_GC

Cuts at:      0     199    1032 
   Size:        199     833
 
Bme1580I G_kGCm'C

Cuts at:      0      57     448     563     725     742    1022    1032 
   Size:         57     391     115     162      17     280      10

  Fragments arranged by size: 

                 391     280     162     115      57      17      10
 
BmgBI CAC'GTC

Cuts at:      0     171    1032 
   Size:        171     861
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     854    1032 
   Size:        854     178
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     472     860    1032 
   Size:        160     312     388     172

  Fragments arranged by size: 

                 388     312     172     160
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     658    1032 
   Size:        658     374
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     136    1032 
   Size:        136     896
 
BsaBI GATnn'nnATC

Cuts at:      0      22    1032 
   Size:         22    1010
 
BsaHI Gr'CG_yC

Cuts at:      0      80     757    1032 
   Size:         80     677     275

  Fragments arranged by size: 

                 677     275      80
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     571     601     877     907    1032 
   Size:        571      30     276      30     125

  Fragments arranged by size: 

                 571     276     125      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     807     810    1032 
   Size:        807       3     222

  Fragments arranged by size: 

                 807     222       3
 
BseYI C'CCAG_C

Cuts at:      0     504     562     790     829     834    1032 
   Size:        504      58     228      39       5     198

  Fragments arranged by size: 

                 504     228     198      58      39       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193    1032 
   Size:        193     839
 
BsiEI CG_ry'CG

Cuts at:      0     193    1032 
   Size:        193     839
 
BsmI GAATG_Cn'

Cuts at:      0     105    1032 
   Size:        105     927
 
Bsp1286I G_dGCh'C

Cuts at:      0      57     372     448     563     725     742    1022    1032 
   Size:         57     315      76     115     162      17     280      10

  Fragments arranged by size: 

                 315     280     162     115      76      57      17      10
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     130     165    1032 
   Size:        130      35     867

  Fragments arranged by size: 

                 867     130      35
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     122    1032 
   Size:        122     910
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      93    1032 
   Size:         93     939
 
BtgI C'CryG_G

Cuts at:      0     339     716     849     867    1032 
   Size:        339     377     133      18     165

  Fragments arranged by size: 

                 377     339     165     133      18
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     513     615    1032 
   Size:        513     102     417

  Fragments arranged by size: 

                 513     417     102
 
DraI TTT'AAA

Cuts at:      0     261    1032 
   Size:        261     771
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     175    1032 
   Size:        175     857
 
EaeI y'GGCC_r

Cuts at:      0     190    1032 
   Size:        190     842
 
EagI C'GGCC_G

Cuts at:      0     190    1032 
   Size:        190     842
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     669    1032 
   Size:        669     363
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     472     625     860    1032 
   Size:        160     312     153     235     172

  Fragments arranged by size: 

                 312     235     172     160     153
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     310    1032 
   Size:        310     722
 
EcoO109I rG'GnC_Cy

Cuts at:      0     315     444     445    1032 
   Size:        315     129       1     587

  Fragments arranged by size: 

                 587     315     129       1
 
EcoRI G'AATT_C

Cuts at:      0     406    1032 
   Size:        406     626
 
HaeII r_GCGC'y

Cuts at:      0    1013    1032 
   Size:       1013      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     754     786    1032 
   Size:        754      32     246

  Fragments arranged by size: 

                 754     246      32
 
HincII GTy'rAC

Cuts at:      0    1005    1032 
   Size:       1005      27
 
MslI CAynn'nnrTG

Cuts at:      0      91     622    1032 
   Size:         91     531     410

  Fragments arranged by size: 

                 531     410      91
 
MspA1I CmG'CkG

Cuts at:      0     195     341     752     934    1032 
   Size:        195     146     411     182      98

  Fragments arranged by size: 

                 411     195     182     146      98
 
NcoI C'CATG_G

Cuts at:      0     849     867    1032 
   Size:        849      18     165

  Fragments arranged by size: 

                 849     165      18
 
NsiI A_TGCA'T

Cuts at:      0       5     527    1032 
   Size:          5     522     505

  Fragments arranged by size: 

                 522     505       5
 
PasI CC'CwG_GG

Cuts at:      0     250    1032 
   Size:        250     782
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     850    1032 
   Size:        850     182
 
PpuMI rG'GwC_Cy

Cuts at:      0     315    1032 
   Size:        315     717
 
PshAI GACnn'nnGTC

Cuts at:      0     313    1032 
   Size:        313     719
 
PspOMI G'GGCC_C

Cuts at:      0     444    1032 
   Size:        444     588
 
PstI C_TGCA'G

Cuts at:      0     965    1032 
   Size:        965      67
 
PvuII CAG'CTG

Cuts at:      0     934    1032 
   Size:        934      98
 
SacII CC_GC'GG

Cuts at:      0     342    1032 
   Size:        342     690
 
SfcI C'TryA_G

Cuts at:      0     961    1032 
   Size:        961      71
 
SmlI C'TyrA_G

Cuts at:      0     673    1032 
   Size:        673     359
 
StyI C'CwwG_G

Cuts at:      0     849     867    1032 
   Size:        849      18     165

  Fragments arranged by size: 

                 849     165      18
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     898    1032 
   Size:        898     134
 
TatI w'GTAC_w

Cuts at:      0     569    1032 
   Size:        569     463
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     877     909    1032 
   Size:        877      32     123

  Fragments arranged by size: 

                 877     123      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     232    1032 
   Size:        232     800
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      90    1032 
   Size:         90     942
 

 Enzymes that do cut:

     AccI     AcuI     AleI     ApaI     ApoI     BanI    BanII    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BpmI    BpuEI     BsaI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI     BsmI
 Bsp1286I    BspMI   BstAPI    BstXI     BtgI    BtgZI     DraI     DrdI
     EaeI     EagI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII
    Hin4I   HincII     MslI   MspA1I     NcoI     NsiI     PasI    PflMI
    PpuMI    PshAI   PspOMI     PstI    PvuII    SacII     SfcI     SmlI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BglII     BmtI     BplI   Bpu10I    BsaAI    BsaWI
  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     BtsI     ClaI    CspCI   DraIII     EarI  EcoICRI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NspI     PacI     PciI     PfoI     PmeI     PmlI
     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     XbaI     XhoI     XmaI     XmnI     ZraI
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