Restriction map for pF1KB8357
 (Linear) (Six-base) MAPSORT of: KIBB8357.seq  Check: 3622  from: 1  to: 789



KIBB8357.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AatII G_ACGT'C

Cuts at:      0      47     190     789 
   Size:         47     143     599

  Fragments arranged by size: 

                 599     143      47
 
AccI GT'mk_AC

Cuts at:      0      53     789 
   Size:         53     736
 
AhdI GACnn_n'nnGTC

Cuts at:      0     186     789 
   Size:        186     603
 
AleI CACnn'nnGTG

Cuts at:      0     238     789 
   Size:        238     551
 
ApoI r'AATT_y

Cuts at:      0     400     789 
   Size:        400     389
 
AvaI C'yCGr_G

Cuts at:      0     524     789 
   Size:        524     265
 
BamHI G'GATC_C

Cuts at:      0     631     789 
   Size:        631     158
 
BanII G_rGCy'C

Cuts at:      0     200     789 
   Size:        200     589
 
BbvCI CC'TCA_GC

Cuts at:      0     136     789 
   Size:        136     653
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     252     286     789 
   Size:        252      34     503

  Fragments arranged by size: 

                 503     252      34
 
BlpI GC'TnA_GC

Cuts at:      0     553     789 
   Size:        553     236
 
BmgBI CAC'GTC

Cuts at:      0      51     789 
   Size:         51     738
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     653     789 
   Size:        653     136
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     789 
   Size:        196     593
 
Bpu10I CC'TnA_GC

Cuts at:      0     136     206     789 
   Size:        136      70     583

  Fragments arranged by size: 

                 583     136      70
 
BsaBI GATnn'nnATC

Cuts at:      0     280     789 
   Size:        280     509
 
BsaHI Gr'CG_yC

Cuts at:      0      44     187     789 
   Size:         44     143     602

  Fragments arranged by size: 

                 602     143      44
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      28     789 
   Size:         28     761
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     789 
   Size:         19     770
 
BseYI C'CCAG_C

Cuts at:      0     514     761     776     789 
   Size:        514     247      15      13

  Fragments arranged by size: 

                 514     247      15      13
 
BsiHKAI G_wGCw'C

Cuts at:      0      29     200     675     789 
   Size:         29     171     475     114

  Fragments arranged by size: 

                 475     171     114      29
 
Bsp1286I G_dGCh'C

Cuts at:      0      29     200     675     789 
   Size:         29     171     475     114

  Fragments arranged by size: 

                 475     171     114      29
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     254     789 
   Size:        254     535
 
BsrBI CCG'CTC

Cuts at:      0      11     789 
   Size:         11     778
 
BsrDI GCAATG_nn'

Cuts at:      0     361     789 
   Size:        361     428
 
BsrFI r'CCGG_y

Cuts at:      0     131     789 
   Size:        131     658
 
BstYI r'GATC_y

Cuts at:      0     631     789 
   Size:        631     158
 
BtgI C'CryG_G

Cuts at:      0      67      81     789 
   Size:         67      14     708

  Fragments arranged by size: 

                 708      67      14
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     468     789 
   Size:        468     321
 
BtsI GCAGTG_nn'

Cuts at:      0     143     400     704     789 
   Size:        143     257     304      85

  Fragments arranged by size: 

                 304     257     143      85
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      49     789 
   Size:         49     740
 
EaeI y'GGCC_r

Cuts at:      0     129     685     789 
   Size:        129     556     104

  Fragments arranged by size: 

                 556     129     104
 
EcoICRI GAG'CTC

Cuts at:      0     198     789 
   Size:        198     591
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     789 
   Size:        196     593
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     572     789 
   Size:        572     217
 
EcoO109I rG'GnC_Cy

Cuts at:      0     210     433     789 
   Size:        210     223     356

  Fragments arranged by size: 

                 356     223     210
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     103     135     172     204     623     655     789 
   Size:        103      32      37      32     419      32     134

  Fragments arranged by size: 

                 419     134     103      37      32      32      32
 
MscI TGG'CCA

Cuts at:      0     687     789 
   Size:        687     102
 
MslI CAynn'nnrTG

Cuts at:      0     238     789 
   Size:        238     551
 
MspA1I CmG'CkG

Cuts at:      0      69     108     238     514     789 
   Size:         69      39     130     276     275

  Fragments arranged by size: 

                 276     275     130      69      39
 
NaeI GCC'GGC

Cuts at:      0     133     789 
   Size:        133     656
 
NgoMIV G'CCGG_C

Cuts at:      0     131     789 
   Size:        131     658
 
PasI CC'CwG_GG

Cuts at:      0     574     607     789 
   Size:        574      33     182

  Fragments arranged by size: 

                 574     182      33
 
PvuII CAG'CTG

Cuts at:      0     108     238     514     789 
   Size:        108     130     276     275

  Fragments arranged by size: 

                 276     275     130     108
 
SacI G_AGCT'C

Cuts at:      0     200     789 
   Size:        200     589
 
SacII CC_GC'GG

Cuts at:      0      70     789 
   Size:         70     719
 
SfcI C'TryA_G

Cuts at:      0      87     737     789 
   Size:         87     650      52

  Fragments arranged by size: 

                 650      87      52
 
StyI C'CwwG_G

Cuts at:      0     429     789 
   Size:        429     360
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     347     372     789 
   Size:        347      25     417

  Fragments arranged by size: 

                 417     347      25
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     773     789 
   Size:        773      16
 
ZraI GAC'GTC

Cuts at:      0      45     188     789 
   Size:         45     143     601

  Fragments arranged by size: 

                 601     143      45
 

 Enzymes that do cut:

    AatII     AccI     AhdI     AleI     ApoI     AvaI    BamHI    BanII
    BbvCI     BcgI     BlpI    BmgBI     BmrI     BpmI   Bpu10I    BsaBI
    BsaHI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BspMI    BsrBI
    BsrDI    BsrFI    BstYI     BtgI    BtgZI     BtsI     DrdI     EaeI
  EcoICRI  Eco57MI    EcoNI EcoO109I    Hin4I     MscI     MslI   MspA1I
     NaeI   NgoMIV     PasI    PvuII     SacI    SacII     SfcI     StyI
    TaqII     XcmI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI     BanI     BbeI     BbsI    BciVI     BclI    BfrBI
     BglI    BglII Bme1580I     BmtI     BplI    BpuEI     BsaI    BsaAI
    BsaWI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI     DraI   DraIII     EagI     EarI     EciI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI
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