(Circular) (Six-base) MAPSORT of: pF1KB8344.seq Check: 7934 from: 1 to: 4016 pF1KB8344 4016 bp With 182 enzymes: SgfI * June 20, 2008 10:28 .. AccI GT'mk_AC Cuts at: 1054 1054 Size: 4016 Acc65I G'GTAC_C Cuts at: 1032 1032 Size: 4016 AclI AA'CG_TT Cuts at: 3856 3856 Size: 4016 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 490 1841 2273 3020 3288 490 Size: 1351 432 747 268 1218 Fragments arranged by size: 1351 1218 747 432 268 AflIII A'CryG_T Cuts at: 976 1368 2472 3449 976 Size: 392 1104 977 1543 Fragments arranged by size: 1543 1104 977 392 AgeI A'CCGG_T Cuts at: 2404 2404 Size: 4016 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 247 247 Size: 4016 AlwNI CAG_nnn'CTG Cuts at: 244 1321 2888 244 Size: 1077 1567 1372 Fragments arranged by size: 1567 1372 1077 ApaI G_GGCC'C Cuts at: 575 575 Size: 4016 ApaLI G'TGCA_C Cuts at: 2786 2786 Size: 4016 ApoI r'AATT_y Cuts at: 916 1020 3369 3625 916 Size: 104 2349 256 1307 Fragments arranged by size: 2349 1307 256 104 AseI AT'TA_AT Cuts at: 20 20 Size: 4016 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4016 AvaI C'yCGr_G Cuts at: 1036 1036 Size: 4016 BamHI G'GATC_C Cuts at: 1041 1041 Size: 4016 BanI G'GyrC_C Cuts at: 305 775 1032 1446 1694 1729 305 Size: 470 257 414 248 35 2592 Fragments arranged by size: 2592 470 414 257 248 35 BanII G_rGCy'C Cuts at: 575 766 1030 2060 575 Size: 191 264 1030 2531 Fragments arranged by size: 2531 1030 264 191 BbeI G_GCGC'C Cuts at: 309 779 1450 1698 309 Size: 470 671 248 2627 Fragments arranged by size: 2627 671 470 248 BciVI GTATCCnnnnn_n' Cuts at: 1914 2675 4016 1914 Size: 761 1341 1914 Fragments arranged by size: 1914 1341 761 BclI T'GATC_A Cuts at: 293 368 1536 293 Size: 75 1168 2773 Fragments arranged by size: 2773 1168 75 BglII A'GATC_T Cuts at: 596 1531 3568 596 Size: 935 2037 1044 Fragments arranged by size: 2037 1044 935 BlpI GC'TnA_GC Cuts at: 245 1128 245 Size: 883 3133 Bme1580I G_kGCm'C Cuts at: 216 575 1641 1734 2790 216 Size: 359 1066 93 1056 1442 Fragments arranged by size: 1442 1066 1056 359 93 BmrI ACTGGGnnnn_n' Cuts at: 1228 1309 1644 3788 1228 Size: 81 335 2144 1456 Fragments arranged by size: 2144 1456 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2383 2383 Size: 4016 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 424 585 895 424 Size: 161 310 3545 Fragments arranged by size: 3545 310 161 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1192 2071 2563 2861 1192 Size: 879 492 298 2347 Fragments arranged by size: 2347 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3720 3720 Size: 4016 BsaAI yAC'GTr Cuts at: 977 1369 977 Size: 392 3624 BsaBI GATnn'nnATC Cuts at: 6 1530 3567 6 Size: 1524 2037 455 Fragments arranged by size: 2037 1524 455 BsaHI Gr'CG_yC Cuts at: 306 776 1447 1695 2397 3893 306 Size: 470 671 248 702 1496 429 Fragments arranged by size: 1496 702 671 470 429 248 BsaWI w'CCGG_w Cuts at: 527 1205 1726 2404 2678 2825 3236 527 Size: 678 521 678 274 147 411 1307 Fragments arranged by size: 1307 678 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 331 361 331 Size: 30 3986 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 355 427 430 355 Size: 72 3 3941 Fragments arranged by size: 3941 72 3 BseYI C'CCAG_C Cuts at: 132 216 735 870 1441 2199 2776 3272 132 Size: 84 519 135 571 758 577 496 876 Fragments arranged by size: 876 758 577 571 519 496 135 84 BsiEI CG_ry'CG Cuts at: 104 1558 1604 2812 104 Size: 1454 46 1208 1308 Fragments arranged by size: 1454 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 766 1030 1808 1998 2790 766 Size: 264 778 190 792 1992 Fragments arranged by size: 1992 792 778 264 190 BsmI GAATG_Cn' Cuts at: 680 804 680 Size: 124 3892 Bsp1286I G_dGCh'C Cuts at: 216 575 766 1030 1641 1734 1808 1998 2060 Size: 359 191 264 611 93 74 190 62 Cuts at: 2060 2790 216 Size: 730 1442 Fragments arranged by size: 1442 730 611 359 264 191 190 93 74 62 BspEI T'CCGG_A Cuts at: 3236 3236 Size: 4016 BspHI T'CATG_A Cuts at: 3192 3192 Size: 4016 BspMI ACCTGCnnnn'nnnn_ Cuts at: 383 576 1066 1280 1582 1963 383 Size: 193 490 214 302 381 2436 Fragments arranged by size: 2436 490 381 302 214 193 BsrBI CCG'CTC Cuts at: 50 2308 2362 3846 4011 50 Size: 2258 54 1484 165 55 Fragments arranged by size: 2258 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1928 1928 Size: 4016 BsrFI r'CCGG_y Cuts at: 2014 2195 2404 2014 Size: 181 209 3626 Fragments arranged by size: 3626 209 181 BssSI C'ACGA_G Cuts at: 187 2287 2645 187 Size: 2100 358 1558 Fragments arranged by size: 2100 1558 358 BstAPI GCAn_nnn'nTGC Cuts at: 716 1393 716 Size: 677 3339 BstBI TT'CG_AA Cuts at: 2377 2377 Size: 4016 BstXI CCAn_nnnn'nTGG Cuts at: 919 919 Size: 4016 BstYI r'GATC_y Cuts at: 1 596 1041 1508 1531 1866 2112 3124 3210 Size: 595 445 467 23 335 246 1012 86 Cuts at: 3210 3222 3568 1 Size: 12 346 449 Fragments arranged by size: 1012 595 467 449 445 346 335 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1966 2147 2310 1966 Size: 181 163 3672 Fragments arranged by size: 3672 181 163 BtsI GCAGTG_nn' Cuts at: 171 352 1259 171 Size: 181 907 2928 Fragments arranged by size: 2928 907 181 DraI TTT'AAA Cuts at: 1015 1015 Size: 4016 DrdI GACnn_nn'nnGTC Cuts at: 393 1722 2580 393 Size: 1329 858 1829 Fragments arranged by size: 1829 1329 858 EaeI y'GGCC_r Cuts at: 1601 1775 2166 2193 3538 1601 Size: 174 391 27 1345 2079 Fragments arranged by size: 2079 1345 391 174 27 EagI C'GGCC_G Cuts at: 1601 1601 Size: 4016 EarI CTCTTCn'nnn_ Cuts at: 404 503 539 2039 2249 404 Size: 99 36 1500 210 2171 Fragments arranged by size: 2171 1500 210 99 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2224 2534 2680 3826 2224 Size: 310 146 1146 2414 Fragments arranged by size: 2414 1146 310 146 EcoICRI GAG'CTC Cuts at: 764 1028 764 Size: 264 3752 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 424 490 585 895 1841 2273 3020 3288 424 Size: 66 95 310 946 432 747 268 1152 Fragments arranged by size: 1152 946 747 432 310 268 95 66 EcoO109I rG'GnC_Cy Cuts at: 395 571 572 1155 395 Size: 176 1 583 3256 Fragments arranged by size: 3256 583 176 1 EcoRI G'AATT_C Cuts at: 1020 1020 Size: 4016 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 960 2035 960 Size: 1075 2941 FspI TGC'GCA Cuts at: 1797 1797 Size: 4016 HaeII r_GCGC'y Cuts at: 309 779 1450 1698 2720 3699 309 Size: 470 671 248 1022 979 626 Fragments arranged by size: 1022 979 671 626 470 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1858 1888 1890 1920 1858 Size: 30 2 30 3954 Fragments arranged by size: 3954 30 30 2 HincII GTy'rAC Cuts at: 273 1055 273 Size: 782 3234 HindIII A'AGCT_T Cuts at: 902 902 Size: 4016 HpaI GTT'AAC Cuts at: 273 273 Size: 4016 KasI G'GCGC_C Cuts at: 305 775 1446 1694 305 Size: 470 671 248 2627 Fragments arranged by size: 2627 671 470 248 KpnI G_GTAC'C Cuts at: 1036 1036 Size: 4016 MluI A'CGCG_T Cuts at: 3449 3449 Size: 4016 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 302 2687 2871 302 Size: 2385 184 1447 Fragments arranged by size: 2385 1447 184 MscI TGG'CCA Cuts at: 1777 1777 Size: 4016 MslI CAynn'nnrTG Cuts at: 404 668 2005 2132 404 Size: 264 1337 127 2288 Fragments arranged by size: 2288 1337 264 127 MspA1I CmG'CkG Cuts at: 739 870 1127 1441 1801 2814 3059 739 Size: 131 257 314 360 1013 245 1696 Fragments arranged by size: 1696 1013 360 314 257 245 131 NaeI GCC'GGC Cuts at: 2197 2197 Size: 4016 NarI GG'CG_CC Cuts at: 306 776 1447 1695 306 Size: 470 671 248 2627 Fragments arranged by size: 2627 671 470 248 NgoMIV G'CCGG_C Cuts at: 2195 2195 Size: 4016 NspI r_CATG'y Cuts at: 1069 1569 2476 1069 Size: 500 907 2609 Fragments arranged by size: 2609 907 500 PciI A'CATG_T Cuts at: 2472 2472 Size: 4016 PfoI T'CCnGG_A Cuts at: 440 911 440 Size: 471 3545 PmeI GTTT'AAAC Cuts at: 1015 1015 Size: 4016 PmlI CAC'GTG Cuts at: 977 977 Size: 4016 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3155 3187 3155 Size: 32 3984 PpuMI rG'GwC_Cy Cuts at: 395 395 Size: 4016 PspOMI G'GGCC_C Cuts at: 571 571 Size: 4016 PstI C_TGCA'G Cuts at: 149 240 521 1063 1748 149 Size: 91 281 542 685 2417 Fragments arranged by size: 2417 685 542 281 91 PvuI CG_AT'CG Cuts at: 104 104 Size: 4016 PvuII CAG'CTG Cuts at: 739 870 1441 1801 739 Size: 131 571 360 2954 Fragments arranged by size: 2954 571 360 131 RsrII CG'GwC_CG Cuts at: 2211 2211 Size: 4016 SacI G_AGCT'C Cuts at: 766 1030 766 Size: 264 3752 SalI G'TCGA_C Cuts at: 1053 1053 Size: 4016 SapI GCTCTTCn'nnn_ Cuts at: 2039 2249 2039 Size: 210 3806 SbfI CC_TGCA'GG Cuts at: 1063 1063 Size: 4016 ScaI AGT'ACT Cuts at: 151 3533 151 Size: 3382 634 SfcI C'TryA_G Cuts at: 33 145 156 236 517 1059 1744 2737 2928 Size: 112 11 80 281 542 685 993 191 Cuts at: 2928 33 Size: 1121 Fragments arranged by size: 1121 993 685 542 281 191 112 80 11 SfoI GGC'GCC Cuts at: 307 777 1448 1696 307 Size: 470 671 248 2627 Fragments arranged by size: 2627 671 470 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4016 SmaI CCC'GGG Cuts at: 1038 1038 Size: 4016 SmlI C'TyrA_G Cuts at: 1171 2086 2578 2840 1171 Size: 915 492 262 2347 Fragments arranged by size: 2347 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4016 SphI G_CATG'C Cuts at: 1069 1569 1069 Size: 500 3516 StyI C'CwwG_G Cuts at: 1150 1504 1150 Size: 354 3662 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 215 706 1599 1732 2191 2400 215 Size: 491 893 133 459 209 1831 Fragments arranged by size: 1831 893 491 459 209 133 TatI w'GTAC_w Cuts at: 149 3531 149 Size: 3382 634 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 331 363 3533 3565 331 Size: 32 3170 32 782 Fragments arranged by size: 3170 782 32 32 Tth111I GACn'n_nGTC Cuts at: 1813 1813 Size: 4016 XbaI T'CTAG_A Cuts at: 1047 1047 Size: 4016 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1511 1511 Size: 4016 XmaI C'CCGG_G Cuts at: 1036 1036 Size: 4016 XmnI GAAnn'nnTTC Cuts at: 512 905 512 Size: 393 3623 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI