################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI BamHI BbeI BbrPI BbvCI BclI BfrI BglII BpmI BsaHI BsaI BseRI BsgI BsiEI BsiWI Bsp1407I BspDI BspMI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI HgaI Hin1I HindIII HpaI KasI KpnI KspI MfeI MflI MluI MseI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tru9I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccIII 938 TCCGGA T^CCGGA ApaI 316 GGGCCC GGGCC^C AspHI 552 GTGCTC G[AT]GC[AT]^C AvaI 538 CCCGAG C^[CT]CG[GA]G AviII 203 TGCGCA TGC^GCA AvrII 261 CCTAGG C^CTAGG BalI 392 TGGCCA TGG^CCA BanI 795 GGTGCC G^G[CT][GA]CC BcgI 62 GCAGCCCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 301 GGATAC GTATCC(6/5) BcnI 1038 CCCGG CC^[GC]GG BglI 805 GCCAGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 261 CCTAGG C^CTAGG BlpI 94 GCTGAGC GC^T[ACGT]AGC Bpu1102I 94 GCTGAGC GC^T[ACGT]AGC BsaBI 648 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 938 TCCGGA [AT]^CCGG[AT] BseAI 938 TCCGGA T^CCGGA BsiHKAI 552 GTGCTC G[AT]GC[AT]^C BsmAI 1021 GAGAC GTCTC(1/5) BsmBI 1021 GAGACG CGTCTC(1/5) BsmFI 851 GTCCC GGGAC(10/14) BsmI 814 GCATTC GAATGC(1/-1) BsoBI 538 CCCGAG C^[CT]CG[GA]G BspEI 938 TCCGGA T^CCGGA BsrBI 400 GAGCGG CCGCTC(-3/-3) BsrFI 517 GCCGGC [GA]^CCGG[CT] BsrI 981 ACTGG ACTGG(1/-1) BssSI 555 CTCGTG CACGAG(-5/-1) BstEII 747 GGTCACC G^GT[ACGT]ACC BstPI 747 GGTCACC G^GT[ACGT]ACC CelII 94 GCTGAGC GC^T[ACGT]AGC Cfr10I 517 GCCGGC [GA]^CCGG[CT] DdeI 95 CTGAG C^T[ACGT]AG DraIII 980 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 250 CTCTTC CTCTTC(1/4) EcoO65I 747 GGTCACC G^GT[ACGT]ACC FauI 752 CCCGC CCCGC(4/6) FspI 203 TGCGCA TGC^GCA HincII 592 GTCAAC GT[CT]^[GA]AC HindII 592 GTCAAC GT[CT]^[GA]AC Ksp632I 250 CTCTTC CTCTTC(1/4) MamI 648 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 392 TGGCCA TGG^CCA MroI 938 TCCGGA T^CCGGA MscI 392 TGGCCA TGG^CCA MspA1I 698 CCGCTG C[AC]G^C[GT]G NaeI 517 GCCGGC GCC^GGC NciI 1038 CCCGG CC^[GC]GG NcoI 100 CCATGG C^CATGG NgoMIV 517 GCCGGC G^CCGGC PstI 547 CTGCAG CTGCA^G ScaI 83 AGTACT AGT^ACT SfaNI 326 GATGC GCATC(5/9) SfcI 547 CTGCAG C^T[GA][CT]AG SmlI 580 CTCAAG C^T[CT][GA]AG SphI 947 GCATGC GCATG^C StuI 995 AGGCCT AGG^CCT TfiI 563 GAATC G^A[AT]TC XcmI 511 CCACTCGCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AflIII 128 ACATGT A^C[GA][CT]GT 617 ACATGT AluI 200 AGCT AG^CT 674 AGCT BanII 316 GGGCCC G[GA]GC[CT]^C 837 GGGCTC BbsI 310 GTCTTC GAAGAC(2/6) 679 GTCTTC BfaI 235 CTAG C^TAG 262 CTAG BpuAI 310 GTCTTC GAAGAC(2/6) 679 GTCTTC BsaAI 28 TACGTG [CT]AC^GT[GA] 406 TACGTG BspHI 860 TCATGA T^CATGA 920 TCATGA BspLU11I 128 ACATGT A^CATGT 617 ACATGT DpnII 141 GATC ^GATC 786 GATC DraII 15 AGGCCCT [GA]G^G[ACGT]CC[CT] 542 AGGGCCT DsaI 100 CCATGG C^C[GA][CT]GG 239 CCGTGG EaeI 119 TGGCCG [CT]^GGCC[GA] 392 TGGCCA EcoO109I 15 AGGCCCT [GA]G^G[ACGT]CC[CT] 542 AGGGCCT HaeII 489 GGCGCT [GA]GCGC^[CT] 889 AGCGCC MaeI 235 CTAG C^TAG 262 CTAG MboI 141 GATC ^GATC 786 GATC NdeII 141 GATC ^GATC 786 GATC PleI 601 GAGTC GAGTC(4/5) 715 GAGTC RcaI 860 TCATGA T^CATGA 920 TCATGA Sau3AI 141 GATC ^GATC 786 GATC Tsp509I 331 AATT ^AATT 366 AATT TspRI 550 CAGTG CA[GC]TG[ACGT][ACGT]^ 980 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AfaI AvaII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboII MnlI MslI MspI MvaI MwoI NlaIII NlaIV NspI RsaI Sau96I ScrFI StyI TaqI TseI Tsp45I TthHB8I