Restriction map for pF1KB8340
##################################
# NO CUT ENZYMES (  188 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
AluI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsiHKAI     BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraII       DrdI        DsaI        Eam1105I    
EarI        Eco47III    Eco81I      EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HgaI        
Hin1I       HindIII     HpaI        HphI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MamI        MboII       MfeI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SapI        SbfI        
ScaI        SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StyI        SwaI        Tsp45I      Tsp509I     TspRI       
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI           70  GTCGAC               GT^[AC][GT]AC
AfaI           54  GTAC                 GT^AC
AlwI          206  GATCC                GGATC(4/5)
AlwNI         157  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII          33  GGACC                G^G[AT]CC
BbvI          156  GCAGC                GCAGC(8/12)
BcnI           81  CCCGG                CC^[GC]GG
BpmI           49  CTGGAG               CTGGAG(16/14)
BsgI          201  GTGCAG               GTGCAG(16/14)
BsiEI         110  CGGCCG               CG[GA][CT]^CG
BsrBI          18  GAGCGG               CCGCTC(-3/-3)
BstYI         205  AGATCC               [GA]^GATC[CT]
DpnII         206  GATC                 ^GATC
DraIII         93  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          110  CGGCCG               [CT]^GGCC[GA]
EagI          110  CGGCCG               C^GGCCG
EclXI         110  CGGCCG               C^GGCCG
Eco52I        110  CGGCCG               C^GGCCG
EcoNI         123  CCTTTCCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII          82  CCGG                 C^CGG
HincII         70  GTCGAC               GT[CT]^[GA]AC
HindII         70  GTCGAC               GT[CT]^[GA]AC
HinfI          85  GATTC                G^A[ACGT]TC
HpaII          82  CCGG                 C^CGG
MboI          206  GATC                 ^GATC
MflI          205  AGATCC               [GA]^GATC[CT]
MseI          152  TTAA                 T^TAA
MspI           82  CCGG                 C^CGG
NciI           81  CCCGG                CC^[GC]GG
NdeII         206  GATC                 ^GATC
NlaIV          22  GGCCCC               GG[ACGT]^[ACGT]CC
RsaI           54  GTAC                 GT^AC
SalI           70  GTCGAC               G^TCGAC
Sau3AI        206  GATC                 ^GATC
SexAI          35  ACCAGGT              A^CC[AT]GGT
TaqI           71  TCGA                 T^CGA
TfiI           85  GATTC                G^A[AT]TC
Tru9I         152  TTAA                 T^TAA
TseI          156  GCAGC                G^C[AT]GC
TthHB8I        71  TCGA                 T^CGA
XhoII         205  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AccII          15  CGCG                 CG^CG
              108  CGCG                
BsaI           10  GAGACC               GGTCTC(1/5)
              136  GAGACC              
BsaJI         113  CCGAGG               C^C[ACGT][ACGT]GG
              128  CCGAGG              
BseRI         130  GAGGAG               GAGGAG(10/8)
              133  GAGGAG              
BsmAI          10  GAGAC                GTCTC(1/5)
              136  GAGAC               
BstUI          15  CGCG                 CG^CG
              108  CGCG                
CfoI          159  GCGC                 GCG^C
              177  GCGC                
Cfr13I         22  GGCCC                G^G[ACGT]CC
               33  GGACC               
DdeI          180  CTCAG                C^T[ACGT]AG
              195  CTCAG               
HaeII         158  AGCGCC               [GA]GCGC^[CT]
              176  GGCGCT              
HhaI          159  GCGC                 GCG^C
              177  GCGC                
HinP1I        159  GCGC                 G^CGC
              177  GCGC                
MvnI           15  CGCG                 CG^CG
              108  CGCG                
Sau96I         22  GGCCC                G^G[ACGT]CC
               33  GGACC               
StuI          120  AGGCCT               AGG^CCT
              186  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AciI        BsiYI       BslI        BssKI       BstNI       
Cac8I       EcoRII      Fnu4HI      HaeIII      ItaI        
MnlI        MvaI        MwoI        ScrFI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com