(Circular) (Six-base) MAPSORT of: pF1KB8339.seq Check: 4311 from: 1 to: 4043 pF1KB8339 4043 bp With 182 enzymes: SgfI * June 20, 2008 10:28 .. AccI GT'mk_AC Cuts at: 1081 1081 Size: 4043 Acc65I G'GTAC_C Cuts at: 496 1059 496 Size: 563 3480 AclI AA'CG_TT Cuts at: 1037 3883 1037 Size: 2846 1197 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 914 1868 2300 3047 3315 914 Size: 954 432 747 268 1642 Fragments arranged by size: 1642 954 747 432 268 AfeI AGC'GCT Cuts at: 634 634 Size: 4043 AflIII A'CryG_T Cuts at: 1395 2499 3476 1395 Size: 1104 977 1962 Fragments arranged by size: 1962 1104 977 AgeI A'CCGG_T Cuts at: 2431 2431 Size: 4043 AleI CACnn'nnGTG Cuts at: 475 475 Size: 4043 AlwNI CAG_nnn'CTG Cuts at: 442 520 1348 2915 442 Size: 78 828 1567 1570 Fragments arranged by size: 1570 1567 828 78 ApaLI G'TGCA_C Cuts at: 2813 2813 Size: 4043 ApoI r'AATT_y Cuts at: 1047 3396 3652 1047 Size: 2349 256 1438 Fragments arranged by size: 2349 1438 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4043 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4043 AvaI C'yCGr_G Cuts at: 692 1063 692 Size: 371 3672 AvrII C'CTAG_G Cuts at: 646 646 Size: 4043 BamHI G'GATC_C Cuts at: 1068 1068 Size: 4043 BanI G'GyrC_C Cuts at: 343 496 998 1059 1473 1721 1756 343 Size: 153 502 61 414 248 35 2630 Fragments arranged by size: 2630 502 414 248 153 61 35 BanII G_rGCy'C Cuts at: 541 714 990 1057 2087 541 Size: 173 276 67 1030 2497 Fragments arranged by size: 2497 1030 276 173 67 BbeI G_GCGC'C Cuts at: 347 1477 1725 347 Size: 1130 248 2665 Fragments arranged by size: 2665 1130 248 BbsI GAAGACnn'nnnn_ Cuts at: 191 249 191 Size: 58 3985 BciVI GTATCCnnnnn_n' Cuts at: 1941 2702 4043 1941 Size: 761 1341 1941 Fragments arranged by size: 1941 1341 761 BclI T'GATC_A Cuts at: 1563 1563 Size: 4043 BglII A'GATC_T Cuts at: 957 1558 3595 957 Size: 601 2037 1405 Fragments arranged by size: 2037 1405 601 BlpI GC'TnA_GC Cuts at: 713 1155 713 Size: 442 3601 Bme1580I G_kGCm'C Cuts at: 1668 1761 2817 1668 Size: 93 1056 2894 Fragments arranged by size: 2894 1056 93 BmgBI CAC'GTC Cuts at: 936 936 Size: 4043 BmrI ACTGGGnnnn_n' Cuts at: 188 1255 1336 1671 3815 188 Size: 1067 81 335 2144 416 Fragments arranged by size: 2144 1067 416 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2410 2410 Size: 4043 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1219 2098 2590 2888 1219 Size: 879 492 298 2374 Fragments arranged by size: 2374 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3747 3747 Size: 4043 BsaAI yAC'GTr Cuts at: 1396 1396 Size: 4043 BsaBI GATnn'nnATC Cuts at: 6 1557 3594 6 Size: 1551 2037 455 Fragments arranged by size: 2037 1551 455 BsaHI Gr'CG_yC Cuts at: 344 1474 1722 2424 3920 344 Size: 1130 248 702 1496 467 Fragments arranged by size: 1496 1130 702 467 248 BsaWI w'CCGG_w Cuts at: 1232 1753 2431 2705 2852 3263 1232 Size: 521 678 274 147 411 2012 Fragments arranged by size: 2012 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 466 552 1468 2226 2803 3299 466 Size: 86 916 758 577 496 1210 Fragments arranged by size: 1210 916 758 577 496 86 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 172 188 787 172 Size: 16 599 3428 Fragments arranged by size: 3428 599 16 BsiEI CG_ry'CG Cuts at: 104 1585 1631 2839 104 Size: 1481 46 1208 1308 Fragments arranged by size: 1481 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 808 990 1057 1835 2025 2817 808 Size: 182 67 778 190 792 2034 Fragments arranged by size: 2034 792 778 190 182 67 BsmBI CGTCTCn'nnnn_ Cuts at: 439 439 Size: 4043 Bsp1286I G_dGCh'C Cuts at: 541 714 808 990 1057 1668 1761 1835 2025 Size: 173 94 182 67 611 93 74 190 Cuts at: 2025 2087 2817 541 Size: 62 730 1767 Fragments arranged by size: 1767 730 611 190 182 173 94 93 74 67 62 BspEI T'CCGG_A Cuts at: 3263 3263 Size: 4043 BspHI T'CATG_A Cuts at: 3219 3219 Size: 4043 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1093 1307 1609 1990 1093 Size: 214 302 381 3146 Fragments arranged by size: 3146 381 302 214 BsrBI CCG'CTC Cuts at: 50 2335 2389 3873 4038 50 Size: 2285 54 1484 165 55 Fragments arranged by size: 2285 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1955 1955 Size: 4043 BsrFI r'CCGG_y Cuts at: 2041 2222 2431 2041 Size: 181 209 3653 Fragments arranged by size: 3653 209 181 BsrGI T'GTAC_A Cuts at: 287 287 Size: 4043 BssSI C'ACGA_G Cuts at: 2314 2672 2314 Size: 358 3685 BstAPI GCAn_nnn'nTGC Cuts at: 1420 1420 Size: 4043 BstBI TT'CG_AA Cuts at: 2404 2404 Size: 4043 BstYI r'GATC_y Cuts at: 1 957 1068 1535 1558 1893 2139 3151 3237 Size: 956 111 467 23 335 246 1012 86 Cuts at: 3237 3249 3595 1 Size: 12 346 449 Fragments arranged by size: 1012 956 467 449 346 335 246 111 86 23 12 BtgI C'CryG_G Cuts at: 1002 1002 Size: 4043 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1993 2174 2337 1993 Size: 181 163 3699 Fragments arranged by size: 3699 181 163 BtsI GCAGTG_nn' Cuts at: 176 482 547 1286 176 Size: 306 65 739 2933 Fragments arranged by size: 2933 739 306 65 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 179 214 179 Size: 35 4008 DraI TTT'AAA Cuts at: 1042 1042 Size: 4043 DrdI GACnn_nn'nnGTC Cuts at: 244 1749 2607 244 Size: 1505 858 1680 Fragments arranged by size: 1680 1505 858 EaeI y'GGCC_r Cuts at: 324 816 1628 1802 2193 2220 3565 324 Size: 492 812 174 391 27 1345 802 Fragments arranged by size: 1345 812 802 492 391 174 27 EagI C'GGCC_G Cuts at: 1628 1628 Size: 4043 EarI CTCTTCn'nnn_ Cuts at: 134 2066 2276 134 Size: 1932 210 1901 Fragments arranged by size: 1932 1901 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2251 2561 2707 3853 2251 Size: 310 146 1146 2441 Fragments arranged by size: 2441 1146 310 146 EcoICRI GAG'CTC Cuts at: 988 1055 988 Size: 67 3976 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 914 1868 2300 3047 3315 914 Size: 954 432 747 268 1642 Fragments arranged by size: 1642 954 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 1182 1182 Size: 4043 EcoRI G'AATT_C Cuts at: 1047 1047 Size: 4043 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 273 2062 273 Size: 1789 2254 FspI TGC'GCA Cuts at: 1824 1824 Size: 4043 HaeII r_GCGC'y Cuts at: 347 636 1477 1725 2747 3726 347 Size: 289 841 248 1022 979 664 Fragments arranged by size: 1022 979 841 664 289 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 734 766 998 1030 1885 1915 1917 1947 734 Size: 32 232 32 855 30 2 30 2830 Fragments arranged by size: 2830 855 232 32 32 30 30 2 HincII GTy'rAC Cuts at: 423 1082 423 Size: 659 3384 HindIII A'AGCT_T Cuts at: 845 845 Size: 4043 KasI G'GCGC_C Cuts at: 343 1473 1721 343 Size: 1130 248 2665 Fragments arranged by size: 2665 1130 248 KpnI G_GTAC'C Cuts at: 500 1063 500 Size: 563 3480 MfeI C'AATT_G Cuts at: 638 638 Size: 4043 MluI A'CGCG_T Cuts at: 3476 3476 Size: 4043 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2714 2898 2714 Size: 184 3859 MscI TGG'CCA Cuts at: 326 818 1804 326 Size: 492 986 2565 Fragments arranged by size: 2565 986 492 MslI CAynn'nnrTG Cuts at: 475 2032 2159 475 Size: 1557 127 2359 Fragments arranged by size: 2359 1557 127 MspA1I CmG'CkG Cuts at: 130 223 318 439 517 924 1154 1468 1828 Size: 93 95 121 78 407 230 314 360 Cuts at: 1828 2841 3086 130 Size: 1013 245 1087 Fragments arranged by size: 1087 1013 407 360 314 245 230 121 95 93 78 NaeI GCC'GGC Cuts at: 2224 2224 Size: 4043 NarI GG'CG_CC Cuts at: 344 1474 1722 344 Size: 1130 248 2665 Fragments arranged by size: 2665 1130 248 NgoMIV G'CCGG_C Cuts at: 2222 2222 Size: 4043 NspI r_CATG'y Cuts at: 1096 1596 2503 1096 Size: 500 907 2636 Fragments arranged by size: 2636 907 500 PasI CC'CwG_GG Cuts at: 534 534 Size: 4043 PciI A'CATG_T Cuts at: 2499 2499 Size: 4043 PfoI T'CCnGG_A Cuts at: 860 860 Size: 4043 PmeI GTTT'AAAC Cuts at: 1042 1042 Size: 4043 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3182 3214 3182 Size: 32 4011 PstI C_TGCA'G Cuts at: 620 905 923 1090 1775 620 Size: 285 18 167 685 2888 Fragments arranged by size: 2888 685 285 167 18 PvuI CG_AT'CG Cuts at: 104 104 Size: 4043 PvuII CAG'CTG Cuts at: 130 223 318 439 517 924 1468 1828 130 Size: 93 95 121 78 407 544 360 2345 Fragments arranged by size: 2345 544 407 360 121 95 93 78 RsrII CG'GwC_CG Cuts at: 2238 2238 Size: 4043 SacI G_AGCT'C Cuts at: 990 1057 990 Size: 67 3976 SalI G'TCGA_C Cuts at: 1080 1080 Size: 4043 SapI GCTCTTCn'nnn_ Cuts at: 2066 2276 2066 Size: 210 3833 SbfI CC_TGCA'GG Cuts at: 1090 1090 Size: 4043 ScaI AGT'ACT Cuts at: 614 3560 614 Size: 2946 1097 SfcI C'TryA_G Cuts at: 33 616 901 919 1086 1771 2764 2955 33 Size: 583 285 18 167 685 993 191 1121 Fragments arranged by size: 1121 993 685 583 285 191 167 18 SfoI GGC'GCC Cuts at: 345 1475 1723 345 Size: 1130 248 2665 Fragments arranged by size: 2665 1130 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4043 SmaI CCC'GGG Cuts at: 1065 1065 Size: 4043 SmlI C'TyrA_G Cuts at: 1198 2113 2605 2867 1198 Size: 915 492 262 2374 Fragments arranged by size: 2374 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4043 SphI G_CATG'C Cuts at: 1096 1596 1096 Size: 500 3543 StyI C'CwwG_G Cuts at: 646 1177 1531 646 Size: 531 354 3158 Fragments arranged by size: 3158 531 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1626 1759 2218 2427 1626 Size: 133 459 209 3242 Fragments arranged by size: 3242 459 209 133 TatI w'GTAC_w Cuts at: 287 511 612 3558 287 Size: 224 101 2946 772 Fragments arranged by size: 2946 772 224 101 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 646 678 3560 3592 646 Size: 32 2882 32 1097 Fragments arranged by size: 2882 1097 32 32 Tth111I GACn'n_nGTC Cuts at: 1840 1840 Size: 4043 XbaI T'CTAG_A Cuts at: 1074 1074 Size: 4043 XcmI CCAnnnn_n'nnnnTGG Cuts at: 474 826 1538 474 Size: 352 712 2979 Fragments arranged by size: 2979 712 352 XmaI C'CCGG_G Cuts at: 1063 1063 Size: 4043 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AflII AhdI AlfI AloI ApaI AscI BaeI BbvCI BcgI BfrBI BglI BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HpaI NcoI NdeI NheI NotI NruI NsiI PacI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI