Restriction map for pF1KB8333
 (Linear) (Six-base) MAPSORT of: KIBB8333.seq  Check: 8553  from: 1  to: 939



KIBB8333.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

Acc65I G'GTAC_C

Cuts at:      0     414     939 
   Size:        414     525
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     622     939 
   Size:        622     317
 
AflIII A'CryG_T

Cuts at:      0     849     851     939 
   Size:        849       2      88

  Fragments arranged by size: 

                 849      88       2
 
AhdI GACnn_n'nnGTC

Cuts at:      0     825     939 
   Size:        825     114
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      88     939 
   Size:         88     851
 
AvaI C'yCGr_G

Cuts at:      0     160     939 
   Size:        160     779
 
BamHI G'GATC_C

Cuts at:      0     636     939 
   Size:        636     303
 
BanI G'GyrC_C

Cuts at:      0      40      91     414     545     939 
   Size:         40      51     323     131     394

  Fragments arranged by size: 

                 394     323     131      51      40
 
BanII G_rGCy'C

Cuts at:      0     434     838     939 
   Size:        434     404     101

  Fragments arranged by size: 

                 434     404     101
 
BbeI G_GCGC'C

Cuts at:      0      95     549     939 
   Size:         95     454     390

  Fragments arranged by size: 

                 454     390      95
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     217     251     939 
   Size:        217      34     688

  Fragments arranged by size: 

                 688     217      34
 
Bme1580I G_kGCm'C

Cuts at:      0     405     534     905     939 
   Size:        405     129     371      34

  Fragments arranged by size: 

                 405     371     129      34
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      30     939 
   Size:         30     909
 
Bpu10I CC'TnA_GC

Cuts at:      0     143     736     939 
   Size:        143     593     203

  Fragments arranged by size: 

                 593     203     143
 
BsaAI yAC'GTr

Cuts at:      0     852     939 
   Size:        852      87
 
BsaHI Gr'CG_yC

Cuts at:      0      92     252     546     939 
   Size:         92     160     294     393

  Fragments arranged by size: 

                 393     294     160      92
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     871     901     939 
   Size:        871      30      38

  Fragments arranged by size: 

                 871      38      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     438     637     939 
   Size:        438     199     302

  Fragments arranged by size: 

                 438     302     199
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     607     939 
   Size:        607     332
 
BsiEI CG_ry'CG

Cuts at:      0     807     939 
   Size:        807     132
 
BsiHKAI G_wGCw'C

Cuts at:      0     838     939 
   Size:        838     101
 
BsmI GAATG_Cn'

Cuts at:      0     319     939 
   Size:        319     620
 
Bsp1286I G_dGCh'C

Cuts at:      0     405     434     534     838     905     939 
   Size:        405      29     100     304      67      34

  Fragments arranged by size: 

                 405     304     100      67      34      29
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     890     939 
   Size:        890      49
 
BsrDI GCAATG_nn'

Cuts at:      0      65     939 
   Size:         65     874
 
BssSI C'ACGA_G

Cuts at:      0     535     939 
   Size:        535     404
 
BstYI r'GATC_y

Cuts at:      0     636     723     939 
   Size:        636      87     216

  Fragments arranged by size: 

                 636     216      87
 
BtgI C'CryG_G

Cuts at:      0      60     549     939 
   Size:         60     489     390

  Fragments arranged by size: 

                 489     390      60
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     367     402     939 
   Size:        367      35     537

  Fragments arranged by size: 

                 537     367      35
 
EaeI y'GGCC_r

Cuts at:      0      18      57     111     423     574     804     939 
   Size:         18      39      54     312     151     230     135

  Fragments arranged by size: 

                 312     230     151     135      54      39      18
 
EagI C'GGCC_G

Cuts at:      0     804     939 
   Size:        804     135
 
EcoICRI GAG'CTC

Cuts at:      0     836     939 
   Size:        836     103
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      30     622     939 
   Size:         30     592     317

  Fragments arranged by size: 

                 592     317      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0     627     939 
   Size:        627     312
 
EcoRV GAT'ATC

Cuts at:      0     354     939 
   Size:        354     585
 
HaeII r_GCGC'y

Cuts at:      0      95     549     939 
   Size:         95     454     390

  Fragments arranged by size: 

                 454     390      95
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     139     171     811     843     939 
   Size:        139      32     640      32      96

  Fragments arranged by size: 

                 640     139      96      32      32
 
HincII GTy'rAC

Cuts at:      0     270     939 
   Size:        270     669
 
KasI G'GCGC_C

Cuts at:      0      91     545     939 
   Size:         91     454     394

  Fragments arranged by size: 

                 454     394      91
 
KpnI G_GTAC'C

Cuts at:      0     418     939 
   Size:        418     521
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     345     939 
   Size:        345     594
 
MscI TGG'CCA

Cuts at:      0      20     425     939 
   Size:         20     405     514

  Fragments arranged by size: 

                 514     405      20
 
MspA1I CmG'CkG

Cuts at:      0     421     560     939 
   Size:        421     139     379

  Fragments arranged by size: 

                 421     379     139
 
NarI GG'CG_CC

Cuts at:      0      92     546     939 
   Size:         92     454     393

  Fragments arranged by size: 

                 454     393      92
 
NcoI C'CATG_G

Cuts at:      0     549     939 
   Size:        549     390
 
NdeI CA'TA_TG

Cuts at:      0     611     939 
   Size:        611     328
 
PmlI CAC'GTG

Cuts at:      0     852     939 
   Size:        852      87
 
PpuMI rG'GwC_Cy

Cuts at:      0     627     939 
   Size:        627     312
 
PstI C_TGCA'G

Cuts at:      0     467     635     939 
   Size:        467     168     304

  Fragments arranged by size: 

                 467     304     168
 
PvuII CAG'CTG

Cuts at:      0     421     560     939 
   Size:        421     139     379

  Fragments arranged by size: 

                 421     379     139
 
SacI G_AGCT'C

Cuts at:      0     838     939 
   Size:        838     101
 
SbfI CC_TGCA'GG

Cuts at:      0     635     939 
   Size:        635     304
 
SfcI C'TryA_G

Cuts at:      0     463     631     939 
   Size:        463     168     308

  Fragments arranged by size: 

                 463     308     168
 
SfoI GGC'GCC

Cuts at:      0      93     547     939 
   Size:         93     454     392

  Fragments arranged by size: 

                 454     392      93
 
StyI C'CwwG_G

Cuts at:      0     549     939 
   Size:        549     390
 
XbaI T'CTAG_A

Cuts at:      0     255     939 
   Size:        255     684
 
XmnI GAAnn'nnTTC

Cuts at:      0     652     939 
   Size:        652     287
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AhdI     AlfI     AvaI    BamHI     BanI
    BanII     BbeI     BcgI Bme1580I     BpmI   Bpu10I    BsaAI    BsaHI
    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI
    BsrDI    BssSI    BstYI     BtgI    CspCI     EaeI     EagI  EcoICRI
  Eco57MI EcoO109I    EcoRV    HaeII    Hin4I   HincII     KasI     KpnI
     MmeI     MscI   MspA1I     NarI     NcoI     NdeI     PmlI    PpuMI
     PstI    PvuII     SacI     SbfI     SfcI     SfoI     StyI     XbaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AleI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI
    BsaBI    BsaWI    BseYI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EarI
     EciI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI  HindIII
     HpaI     MfeI     MluI     MslI     NaeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XcmI
     XhoI     XmaI     ZraI
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