Restriction map for pF1KB8332
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

AatII       AccII       AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp700I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BbrPI       BbvCI       BcgI        BclI        BcnI        
BfrI        BlnI        BlpI        Bpu1102I    BsaI        
BsiEI       BsiWI       BsmBI       BsmI        BspDI       
BspLU11I    BsrBI       BsrFI       BssHII      BssKI       
BstAPI      BstBI       BstNI       BstUI       Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoRI       EcoRII      EcoRV       
EcoT22I     FbaI        FseI        FspI        HgaI        
HpaI        KspI        MfeI        MluI        MluNI       
MscI        MunI        MvaI        MvnI        NaeI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PstI        PvuI        RsrII       SacII       
SapI        SbfI        ScaI        ScrFI       SexAI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspI        StuI        SwaI        
Tth111I     XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   72 enzymes) #
###################################

Acc65I       1599  GGTACC               G^GTACC
AccIII        795  TCCGGA               T^CCGGA
AcyI          301  GGCGCC               G[GA]^CG[CT]C
AlwI          310  GGATC                GGATC(4/5)
AseI         1783  ATTAAT               AT^TAAT
AsnI         1783  ATTAAT               AT^TAAT
Asp718I      1599  GGTACC               G^GTACC
AvaI          371  CTCGAG               C^[CT]CG[GA]G
BanII         626  GAGCTC               G[GA]GC[CT]^C
BbeI          301  GGCGCC               GGCGC^C
BbsI          679  GAAGAC               GAAGAC(2/6)
BciVI         792  GTATCC               GTATCC(6/5)
BfaI          314  CTAG                 C^TAG
BglI         1301  GCCGCCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         153  AGATCT               A^GATCT
BpuAI         679  GAAGAC               GAAGAC(2/6)
BsaAI         384  CACGTA               [CT]AC^GT[GA]
BsaHI         301  GGCGCC               G[GA]^CG[CT]C
BsaWI         795  TCCGGA               [AT]^CCGG[AT]
BseAI         795  TCCGGA               T^CCGGA
BsgI         1055  CTGCAC               GTGCAG(16/14)
BsmAI         240  GTCTC                GTCTC(1/5)
BsmFI         226  GTCCC                GGGAC(10/14)
BsoBI         371  CTCGAG               C^[CT]CG[GA]G
Bsp1407I     1289  TGTACA               T^GTACA
BspEI         795  TCCGGA               T^CCGGA
BsrGI        1289  TGTACA               T^GTACA
BssSI         805  CTCGTG               CACGAG(-5/-1)
Bst1107I      387  GTATAC               GTA^TAC
BstEII       1517  GGTCACC              G^GT[ACGT]ACC
BstPI        1517  GGTCACC              G^GT[ACGT]ACC
BstXI         285  CCAAGACTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       387  GTATAC               GTA^TAC
CfoI          302  GCGC                 GCG^C
DraI          498  TTTAAA               TTT^AAA
DraII         321  AGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI         1224  GACATCTATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         1764  TGGCCG               [CT]^GGCC[GA]
EcoO109I      321  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      1517  GGTCACC              G^GT[ACGT]ACC
EcoT14I      1305  CCATGG               C^C[AT][AT]GG
FauI         1739  CCCGC                CCCGC(4/6)
HaeII         301  GGCGCC               [GA]GCGC^[CT]
HapII         796  CCGG                 C^CGG
HhaI          302  GCGC                 GCG^C
Hin1I         301  GGCGCC               G[GA]^CG[CT]C
HinP1I        302  GCGC                 G^CGC
HindIII      1441  AAGCTT               A^AGCTT
HpaII         796  CCGG                 C^CGG
KasI          301  GGCGCC               G^GCGCC
KpnI         1599  GGTACC               GGTAC^C
MaeI          314  CTAG                 C^TAG
MroI          795  TCCGGA               T^CCGGA
MspI          796  CCGG                 C^CGG
NarI          301  GGCGCC               GG^CGCC
NcoI         1305  CCATGG               C^CATGG
PaeR7I        371  CTCGAG               C^TCGAG
PflMI        1354  CCAATCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         321  AGGACCT              [GA]G^G[AT]CC[CT]
PshBI        1783  ATTAAT               AT^TAAT
SacI          626  GAGCTC               GAGCT^C
SalI          358  GTCGAC               G^TCGAC
SfcI          660  CTACAG               C^T[GA][CT]AG
SfoI          301  GGCGCC               GGC^GCC
SmlI          371  CTCGAG               C^T[CT][GA]AG
SphI         1297  GCATGC               GCATG^C
SspBI        1289  TGTACA               T^GTACA
StyI         1305  CCATGG               C^C[AT][AT]GG
Van91I       1354  CCAATCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          313  TCTAGA               T^CTAGA
XcmI         1610  CCAGCATTTCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          371  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AciI         1302  CCGC                 CCGC(-3/-1)
             1740  CCGC                
AspHI         261  GAGCAC               G[AT]GC[AT]^C
              626  GAGCTC              
BanI          301  GGCGCC               G^G[CT][GA]CC
             1599  GGTACC              
BsaBI         642  GATTAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1116  GATTAGCATC          
BsaJI         305  CCGTGG               C^C[ACGT][ACGT]GG
             1305  CCATGG              
BsiHKAI       261  GAGCAC               G[AT]GC[AT]^C
              626  GAGCTC              
BspHI         537  TCATGA               T^CATGA
              840  TCATGA              
BspMI        1830  ACCTGC               ACCTGC(4/8)
              703  GCAGGT              
BsrDI         185  CATTGC               GCAATG(2/0)
             1742  GCAATG              
BstYI         153  AGATCT               [GA]^GATC[CT]
              310  GGATCT              
Cac8I        1297  GCATGC               GC[ACGT]^[ACGT]GC
             1609  GCCAGC              
DpnII         154  GATC                 ^GATC
              311  GATC                
DsaI          305  CCGTGG               C^C[GA][CT]GG
             1305  CCATGG              
HincII        358  GTCGAC               GT[CT]^[GA]AC
             1252  GTCAAC              
HindII        358  GTCGAC               GT[CT]^[GA]AC
             1252  GTCAAC              
HphI         1684  GGTGA                GGTGA(8/7)
             1519  TCACC               
MaeII          36  ACGT                 A^CGT
              385  ACGT                
MamI          642  GATTAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1116  GATTAGCATC          
MboI          154  GATC                 ^GATC
              311  GATC                
MflI          153  AGATCT               [GA]^GATC[CT]
              310  GGATCT              
MslI         1369  CATTGTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1614  CATTTCTGTG          
NdeII         154  GATC                 ^GATC
              311  GATC                
NspI         1160  ACATGC               [GA]CATG^[CT]
             1297  GCATGC              
RcaI          537  TCATGA               T^CATGA
              840  TCATGA              
Sau3AI        154  GATC                 ^GATC
              311  GATC                
XhoII         153  AGATCT               [GA]^GATC[CT]
              310  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AccI        AcsI        AfaI        AluI        AlwNI       
ApoI        AvaII       BbvI        BmyI        BpmI        
BseRI       BsiYI       BslI        Bsp1286I    BsrI        
BstF5I      Cfr13I      DdeI        EarI        Fnu4HI      
FokI        HaeIII      HinfI       ItaI        Ksp632I     
MaeIII      MboII       MnlI        MseI        MspA1I      
MwoI        NlaIII      NlaIV       PleI        PvuII       
RsaI        Sau96I      SfaNI       TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     
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