################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvrII BalI BbrPI BbsI BbvCI BciVI BclI BfrI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HgaI HincII HindII HindIII HinfI HpaI KpnI Ksp632I MaeII MaeIII MamI MboII MfeI MluI MluNI MroI MscI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SfcI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 182 CGCG CG^CG AcyI 175 GGCGCC G[GA]^CG[CT]C AfaI 542 GTAC GT^AC Asp700I 369 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 210 TGCGCA TGC^GCA BanII 569 GAGCCC G[GA]GC[CT]^C BbeI 175 GGCGCC GGCGC^C BbvI 148 GCAGC GCAGC(8/12) BfaI 494 CTAG C^TAG BglII 498 AGATCT A^GATCT BsaHI 175 GGCGCC G[GA]^CG[CT]C BsaI 534 GAGACC GGTCTC(1/5) BseRI 170 GAGGAG GAGGAG(10/8) BsgI 585 GTGCAG GTGCAG(16/14) BsmAI 534 GAGAC GTCTC(1/5) BsmI 65 GCATTC GAATGC(1/-1) BsrBI 343 CCGCTC CCGCTC(-3/-3) BsrDI 86 GCAATG GCAATG(2/0) BstUI 182 CGCG CG^CG BstXI 573 CCAGGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EcoT14I 296 CCATGG C^C[AT][AT]GG FspI 210 TGCGCA TGC^GCA Hin1I 175 GGCGCC G[GA]^CG[CT]C HphI 377 TCACC GGTGA(8/7) KasI 175 GGCGCC G^GCGCC KspI 181 CCGCGG CCGC^GG MaeI 494 CTAG C^TAG MseI 602 TTAA T^TAA MspA1I 181 CCGCGG C[AC]G^C[GT]G MvnI 182 CGCG CG^CG NarI 175 GGCGCC GG^CGCC NcoI 296 CCATGG C^CATGG PpuMI 129 AGGTCCC [GA]G^G[AT]CC[CT] RsaI 542 GTAC GT^AC SacII 181 CCGCGG CCGC^GG SexAI 537 ACCTGGT A^CC[AT]GGT SfiI 49 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 175 GGCGCC GGC^GCC SmlI 262 CTTGAG C^T[CT][GA]AG StuI 521 AGGCCT AGG^CCT StyI 296 CCATGG C^C[AT][AT]GG TaqI 31 TCGA T^CGA Tru9I 602 TTAA T^TAA TseI 148 GCAGC G^C[AT]GC TthHB8I 31 TCGA T^CGA XmnI 369 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AluI 308 AGCT AG^CT 492 AGCT BamHI 320 GGATCC G^GATCC 466 GGATCC BcgI 151 GCAAGGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 587 GCAGAGGTGTCG BglI 50 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 77 GCCCAGGGGGC DdeI 502 CTCAG C^T[ACGT]AG 525 CTCAG HaeII 175 GGCGCC [GA]GCGC^[CT] 430 GGCGCT MslI 193 CATGGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 273 CATCACGATG NlaIII 193 CATG CATG^ 297 CATG SmaI 134 CCCGGG CCC^GGG 329 CCCGGG XmaCI 134 CCCGGG C^CCGGG 329 CCCGGG XmaI 134 CCCGGG C^CCGGG 329 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AlwI AvaI AvaII BanI BcnI BmyI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DpnII DraII DsaI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MboI MflI MnlI MspI MvaI MwoI NciI NdeII NlaIV Sau3AI Sau96I ScrFI SfaNI XhoII