(Circular) (Six-base) MAPSORT of: pF1KB8331.seq Check: 943 from: 1 to: 3743 pF1KB8331 3743 bp With 182 enzymes: SgfI * July 2, 2008 13:23 .. AccI GT'mk_AC Cuts at: 781 781 Size: 3743 Acc65I G'GTAC_C Cuts at: 759 759 Size: 3743 AclI AA'CG_TT Cuts at: 3583 3583 Size: 3743 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 496 1568 2000 2747 3015 496 Size: 1072 432 747 268 1224 Fragments arranged by size: 1224 1072 747 432 268 AflIII A'CryG_T Cuts at: 1095 2199 3176 1095 Size: 1104 977 1662 Fragments arranged by size: 1662 1104 977 AgeI A'CCGG_T Cuts at: 2131 2131 Size: 3743 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 323 323 Size: 3743 AlwNI CAG_nnn'CTG Cuts at: 1048 2615 1048 Size: 1567 2176 ApaLI G'TGCA_C Cuts at: 2513 2513 Size: 3743 ApoI r'AATT_y Cuts at: 747 3096 3352 747 Size: 2349 256 1138 Fragments arranged by size: 2349 1138 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3743 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3743 AvaI C'yCGr_G Cuts at: 242 267 437 670 763 242 Size: 25 170 233 93 3222 Fragments arranged by size: 3222 233 170 93 25 BamHI G'GATC_C Cuts at: 428 574 768 428 Size: 146 194 3403 Fragments arranged by size: 3403 194 146 BanI G'GyrC_C Cuts at: 220 283 307 332 759 1173 1421 1456 220 Size: 63 24 25 427 414 248 35 2507 Fragments arranged by size: 2507 427 414 248 63 35 25 24 BanII G_rGCy'C Cuts at: 681 757 1787 681 Size: 76 1030 2637 Fragments arranged by size: 2637 1030 76 BbeI G_GCGC'C Cuts at: 287 1177 1425 287 Size: 890 248 2605 Fragments arranged by size: 2605 890 248 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 248 282 684 718 90 Size: 34 124 34 402 34 3115 Fragments arranged by size: 3115 402 124 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 1641 2402 3743 1641 Size: 761 1341 1641 Fragments arranged by size: 1641 1341 761 BclI T'GATC_A Cuts at: 1263 1263 Size: 3743 BglI GCCn_nnn'nGGC Cuts at: 112 164 191 112 Size: 52 27 3664 Fragments arranged by size: 3664 52 27 BglII A'GATC_T Cuts at: 606 1258 3295 606 Size: 652 2037 1054 Fragments arranged by size: 2037 1054 652 BlpI GC'TnA_GC Cuts at: 855 855 Size: 3743 Bme1580I G_kGCm'C Cuts at: 130 337 1368 1461 2517 130 Size: 207 1031 93 1056 1356 Fragments arranged by size: 1356 1056 1031 207 93 BmrI ACTGGGnnnn_n' Cuts at: 955 1036 1371 3515 955 Size: 81 335 2144 1183 Fragments arranged by size: 2144 1183 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2110 2110 Size: 3743 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 391 919 1798 2290 2588 391 Size: 528 879 492 298 1546 Fragments arranged by size: 1546 879 528 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 636 3447 636 Size: 2811 932 BsaAI yAC'GTr Cuts at: 1096 1096 Size: 3743 BsaBI GATnn'nnATC Cuts at: 6 1257 3294 6 Size: 1251 2037 455 Fragments arranged by size: 2037 1251 455 BsaHI Gr'CG_yC Cuts at: 284 1174 1422 2124 3620 284 Size: 890 248 702 1496 407 Fragments arranged by size: 1496 890 702 407 248 BsaWI w'CCGG_w Cuts at: 932 1453 2131 2405 2552 2963 932 Size: 521 678 274 147 411 1712 Fragments arranged by size: 1712 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 293 293 Size: 3743 BseYI C'CCAG_C Cuts at: 541 570 1168 1926 2503 2999 541 Size: 29 598 758 577 496 1285 Fragments arranged by size: 1285 758 598 577 496 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 714 714 Size: 3743 BsiEI CG_ry'CG Cuts at: 104 1285 1331 2539 104 Size: 1181 46 1208 1308 Fragments arranged by size: 1308 1208 1181 46 BsiHKAI G_wGCw'C Cuts at: 757 1535 1725 2517 757 Size: 778 190 792 1983 Fragments arranged by size: 1983 792 778 190 BsmI GAATG_Cn' Cuts at: 173 173 Size: 3743 Bsp1286I G_dGCh'C Cuts at: 130 337 681 757 1368 1461 1535 1725 1787 Size: 207 344 76 611 93 74 190 62 Cuts at: 1787 2517 130 Size: 730 1356 Fragments arranged by size: 1356 730 611 344 207 190 93 76 74 62 BspEI T'CCGG_A Cuts at: 2963 2963 Size: 3743 BspHI T'CATG_A Cuts at: 2919 2919 Size: 3743 BspMI ACCTGCnnnn'nnnn_ Cuts at: 793 1007 1309 1690 793 Size: 214 302 381 2846 Fragments arranged by size: 2846 381 302 214 BsrBI CCG'CTC Cuts at: 50 453 2035 2089 3573 3738 50 Size: 403 1582 54 1484 165 55 Fragments arranged by size: 1582 1484 403 165 55 54 BsrDI GCAATG_nn' Cuts at: 201 1655 201 Size: 1454 2289 BsrFI r'CCGG_y Cuts at: 1741 1922 2131 1741 Size: 181 209 3353 Fragments arranged by size: 3353 209 181 BssSI C'ACGA_G Cuts at: 2014 2372 2014 Size: 358 3385 BstAPI GCAn_nnn'nTGC Cuts at: 1120 1120 Size: 3743 BstBI TT'CG_AA Cuts at: 2104 2104 Size: 3743 BstXI CCAn_nnnn'nTGG Cuts at: 688 688 Size: 3743 BstYI r'GATC_y Cuts at: 1 428 574 606 768 1235 1258 1593 1839 Size: 427 146 32 162 467 23 335 246 Cuts at: 1839 2851 2937 2949 3295 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1012 467 449 427 346 335 246 162 146 86 32 23 12 BtgI C'CryG_G Cuts at: 224 289 404 224 Size: 65 115 3563 Fragments arranged by size: 3563 115 65 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 161 1693 1874 2037 161 Size: 1532 181 163 1867 Fragments arranged by size: 1867 1532 181 163 BtsI GCAGTG_nn' Cuts at: 986 986 Size: 3743 DraI TTT'AAA Cuts at: 742 742 Size: 3743 DrdI GACnn_nn'nnGTC Cuts at: 1449 2307 1449 Size: 858 2885 EaeI y'GGCC_r Cuts at: 1328 1502 1893 1920 3265 1328 Size: 174 391 27 1345 1806 Fragments arranged by size: 1806 1345 391 174 27 EagI C'GGCC_G Cuts at: 1328 1328 Size: 3743 EarI CTCTTCn'nnn_ Cuts at: 1766 1976 1766 Size: 210 3533 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 561 567 1951 2261 2407 3553 561 Size: 6 1384 310 146 1146 751 Fragments arranged by size: 1384 1146 751 310 146 6 EcoICRI GAG'CTC Cuts at: 755 755 Size: 3743 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 496 1568 2000 2747 3015 496 Size: 1072 432 747 268 1224 Fragments arranged by size: 1224 1072 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 166 238 263 882 166 Size: 72 25 619 3027 Fragments arranged by size: 3027 619 72 25 EcoRI G'AATT_C Cuts at: 747 747 Size: 3743 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1762 1762 Size: 3743 FspI TGC'GCA Cuts at: 320 1524 320 Size: 1204 2539 HaeII r_GCGC'y Cuts at: 287 542 1177 1425 2447 3426 287 Size: 255 635 248 1022 979 604 Fragments arranged by size: 1022 979 635 604 255 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1585 1615 1617 1647 1585 Size: 30 2 30 3681 Fragments arranged by size: 3681 30 30 2 HincII GTy'rAC Cuts at: 782 782 Size: 3743 KasI G'GCGC_C Cuts at: 283 1173 1421 283 Size: 890 248 2605 Fragments arranged by size: 2605 890 248 KpnI G_GTAC'C Cuts at: 763 763 Size: 3743 MluI A'CGCG_T Cuts at: 3176 3176 Size: 3743 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2414 2598 2414 Size: 184 3559 MscI TGG'CCA Cuts at: 1504 1504 Size: 3743 MslI CAynn'nnrTG Cuts at: 305 385 1732 1859 305 Size: 80 1347 127 2189 Fragments arranged by size: 2189 1347 127 80 MspA1I CmG'CkG Cuts at: 291 854 1168 1528 2541 2786 291 Size: 563 314 360 1013 245 1248 Fragments arranged by size: 1248 1013 563 360 314 245 NaeI GCC'GGC Cuts at: 1924 1924 Size: 3743 NarI GG'CG_CC Cuts at: 284 1174 1422 284 Size: 890 248 2605 Fragments arranged by size: 2605 890 248 NcoI C'CATG_G Cuts at: 404 404 Size: 3743 NgoMIV G'CCGG_C Cuts at: 1922 1922 Size: 3743 NspI r_CATG'y Cuts at: 796 1296 2203 796 Size: 500 907 2336 Fragments arranged by size: 2336 907 500 PasI CC'CwG_GG Cuts at: 187 681 187 Size: 494 3249 PciI A'CATG_T Cuts at: 2199 2199 Size: 3743 PmeI GTTT'AAAC Cuts at: 742 742 Size: 3743 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2882 2914 2882 Size: 32 3711 PpuMI rG'GwC_Cy Cuts at: 238 238 Size: 3743 PstI C_TGCA'G Cuts at: 790 1475 790 Size: 685 3058 PvuI CG_AT'CG Cuts at: 104 104 Size: 3743 PvuII CAG'CTG Cuts at: 1168 1528 1168 Size: 360 3383 RsrII CG'GwC_CG Cuts at: 1938 1938 Size: 3743 SacI G_AGCT'C Cuts at: 757 757 Size: 3743 SacII CC_GC'GG Cuts at: 292 292 Size: 3743 SalI G'TCGA_C Cuts at: 780 780 Size: 3743 SapI GCTCTTCn'nnn_ Cuts at: 1766 1976 1766 Size: 210 3533 SbfI CC_TGCA'GG Cuts at: 790 790 Size: 3743 ScaI AGT'ACT Cuts at: 3260 3260 Size: 3743 SexAI A'CCwGG_T Cuts at: 645 645 Size: 3743 SfcI C'TryA_G Cuts at: 33 786 1471 2464 2655 33 Size: 753 685 993 191 1121 Fragments arranged by size: 1121 993 753 685 191 SfiI GGCCn_nnn'nGGCC Cuts at: 164 164 Size: 3743 SfoI GGC'GCC Cuts at: 285 1175 1423 285 Size: 890 248 2605 Fragments arranged by size: 2605 890 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3743 SmaI CCC'GGG Cuts at: 244 439 765 244 Size: 195 326 3222 Fragments arranged by size: 3222 326 195 SmlI C'TyrA_G Cuts at: 370 898 1813 2305 2567 370 Size: 528 915 492 262 1546 Fragments arranged by size: 1546 915 528 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3743 SphI G_CATG'C Cuts at: 796 1296 796 Size: 500 3243 StuI AGG'CCT Cuts at: 631 631 Size: 3743 StyI C'CwwG_G Cuts at: 404 877 1231 404 Size: 473 354 2916 Fragments arranged by size: 2916 473 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 633 680 1326 1459 1918 2127 633 Size: 47 646 133 459 209 2249 Fragments arranged by size: 2249 646 459 209 133 47 TatI w'GTAC_w Cuts at: 3258 3258 Size: 3743 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 316 348 3260 3292 316 Size: 32 2912 32 767 Fragments arranged by size: 2912 767 32 32 Tth111I GACn'n_nGTC Cuts at: 1540 1540 Size: 3743 XbaI T'CTAG_A Cuts at: 774 774 Size: 3743 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1238 1238 Size: 3743 XmaI C'CCGG_G Cuts at: 242 437 763 242 Size: 195 326 3222 Fragments arranged by size: 3222 326 195 XmnI GAAnn'nnTTC Cuts at: 481 481 Size: 3743 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SanDI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI