################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV FbaI FseI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MseI MunI MvnI NarI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI Tru9I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcyI 1042 GGCGTC G[GA]^CG[CT]C AfaI 948 GTAC GT^AC Alw44I 574 GTGCAC G^TGCAC ApaI 665 GGGCCC GGGCC^C ApaLI 574 GTGCAC G^TGCAC AvaI 565 CCCGAG C^[CT]CG[GA]G AviII 218 TGCGCA TGC^GCA BbsI 624 GTCTTC GAAGAC(2/6) BbvCI 465 CCTCAGC CCTCAGC(-5/-2) BpuAI 624 GTCTTC GAAGAC(2/6) BsaHI 1042 GGCGTC G[GA]^CG[CT]C BsaWI 798 ACCGGA [AT]^CCGG[AT] BsgI 112 GTGCAG GTGCAG(16/14) BsiEI 638 CGGCCG CG[GA][CT]^CG BsoBI 565 CCCGAG C^[CT]CG[GA]G BstAPI 210 GCACACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 849 GGATG GGATG(2/0) BstXI 713 CCAGCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 32 AGATCC [GA]^GATC[CT] EagI 638 CGGCCG C^GGCCG EarI 805 GAAGAG CTCTTC(1/4) EclXI 638 CGGCCG C^GGCCG Eco52I 638 CGGCCG C^GGCCG EcoT22I 295 ATGCAT ATGCA^T FokI 849 GGATG GGATG(9/13) FspI 218 TGCGCA TGC^GCA Hin1I 1042 GGCGTC G[GA]^CG[CT]C Ksp632I 805 GAAGAG CTCTTC(1/4) MflI 32 AGATCC [GA]^GATC[CT] MslI 294 CATGCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 640 GCCGGC GCC^GGC NdeI 298 CATATG CA^TATG NgoMIV 640 GCCGGC G^CCGGC NsiI 295 ATGCAT ATGCA^T PleI 602 GACTC GAGTC(4/5) PpuMI 461 AGGACCT [GA]G^G[AT]CC[CT] RsaI 948 GTAC GT^AC SacI 976 GAGCTC GAGCT^C SapI 805 GAAGAGC GCTCTTC(1/4) SbfI 231 CCTGCAGG CCTGCA^GG SexAI 188 ACCAGGT A^CC[AT]GGT SfaNI 509 GCATC GCATC(5/9) SfiI 174 GGCCAGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 231 CCTGCAGG CCTGCA^GG Tsp509I 860 AATT ^AATT XhoII 32 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwI 33 GATCC GGATC(4/5) 455 GGATC AspHI 574 GTGCAC G[AT]GC[AT]^C 976 GAGCTC BalI 173 TGGCCA TGG^CCA 447 TGGCCA BglI 175 GCCAGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 675 GCCCAAGGGGC BsiHKAI 574 GTGCAC G[AT]GC[AT]^C 976 GAGCTC BsmFI 1057 GGGAC GGGAC(10/14) 786 GTCCC BsrFI 582 GCCGGT [GA]^CCGG[CT] 640 GCCGGC Cfr10I 582 GCCGGT [GA]^CCGG[CT] 640 GCCGGC DsaI 149 CCATGG C^C[GA][CT]GG 450 CCATGG EcoNI 246 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 404 CCTACTGCAGG FauI 529 CCCGC CCCGC(4/6) 81 GCGGG HaeII 135 GGCGCT [GA]GCGC^[CT] 646 AGCGCC HgaI 622 GCGTC GACGC(5/10) 1043 GCGTC HphI 288 GGTGA GGTGA(8/7) 939 GGTGA MaeIII 940 GTGAC ^GT[ACGT]AC 1009 GTCAC MluNI 173 TGGCCA TGG^CCA 447 TGGCCA MscI 173 TGGCCA TGG^CCA 447 TGGCCA NcoI 149 CCATGG C^CATGG 450 CCATGG NspI 221 GCATGT [GA]CATG^[CT] 293 ACATGC PflMI 165 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 883 CCAGGATTTGG TaqI 501 TCGA T^CGA 749 TCGA TfiI 561 GATTC G^A[AT]TC 1064 GATTC Tsp45I 940 GTGAC ^GT[GC]AC 1009 GTCAC TthHB8I 501 TCGA T^CGA 749 TCGA Van91I 165 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 883 CCAGGATTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AluI AlwNI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PstI PvuII Sau3AI Sau96I ScrFI SfcI StyI TseI TspRI