(Circular) (Six-base) MAPSORT of: pF1KB8325.seq Check: 9535 from: 1 to: 3731 pF1KB8325 3731 bp With 182 enzymes: SgfI * June 20, 2008 10:28 .. AccI GT'mk_AC Cuts at: 769 769 Size: 3731 Acc65I G'GTAC_C Cuts at: 747 747 Size: 3731 AclI AA'CG_TT Cuts at: 3571 3571 Size: 3731 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 154 1556 1988 2735 3003 154 Size: 1402 432 747 268 882 Fragments arranged by size: 1402 882 747 432 268 AflIII A'CryG_T Cuts at: 1083 2187 3164 1083 Size: 1104 977 1650 Fragments arranged by size: 1650 1104 977 AgeI A'CCGG_T Cuts at: 2119 2119 Size: 3731 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 312 344 312 Size: 32 3699 AlwNI CAG_nnn'CTG Cuts at: 1036 2603 1036 Size: 1567 2164 ApaLI G'TGCA_C Cuts at: 347 2501 347 Size: 2154 1577 ApoI r'AATT_y Cuts at: 520 735 3084 3340 520 Size: 215 2349 256 911 Fragments arranged by size: 2349 911 256 215 AseI AT'TA_AT Cuts at: 20 20 Size: 3731 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3731 AvaI C'yCGr_G Cuts at: 751 751 Size: 3731 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 380 413 380 Size: 33 3698 BamHI G'GATC_C Cuts at: 756 756 Size: 3731 BanI G'GyrC_C Cuts at: 747 1161 1409 1444 747 Size: 414 248 35 3034 Fragments arranged by size: 3034 414 248 35 BanII G_rGCy'C Cuts at: 745 1775 745 Size: 1030 2701 BbeI G_GCGC'C Cuts at: 1165 1413 1165 Size: 248 3483 BciVI GTATCCnnnnn_n' Cuts at: 288 1629 2390 3731 288 Size: 1341 761 1341 288 Fragments arranged by size: 1341 1341 761 288 BclI T'GATC_A Cuts at: 1251 1251 Size: 3731 BglII A'GATC_T Cuts at: 1246 3283 1246 Size: 2037 1694 BlpI GC'TnA_GC Cuts at: 843 843 Size: 3731 Bme1580I G_kGCm'C Cuts at: 351 1356 1449 2505 351 Size: 1005 93 1056 1577 Fragments arranged by size: 1577 1056 1005 93 BmgBI CAC'GTC Cuts at: 120 120 Size: 3731 BmrI ACTGGGnnnn_n' Cuts at: 943 1024 1359 3503 943 Size: 81 335 2144 1171 Fragments arranged by size: 2144 1171 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2098 2098 Size: 3731 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 204 907 1786 2278 2576 204 Size: 703 879 492 298 1359 Fragments arranged by size: 1359 879 703 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3435 3435 Size: 3731 BsaAI yAC'GTr Cuts at: 1084 1084 Size: 3731 BsaBI GATnn'nnATC Cuts at: 6 142 1245 3282 6 Size: 136 1103 2037 455 Fragments arranged by size: 2037 1103 455 136 BsaHI Gr'CG_yC Cuts at: 1162 1410 2112 3608 1162 Size: 248 702 1496 1285 Fragments arranged by size: 1496 1285 702 248 BsaWI w'CCGG_w Cuts at: 920 1441 2119 2393 2540 2951 920 Size: 521 678 274 147 411 1700 Fragments arranged by size: 1700 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 312 342 312 Size: 30 3701 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 707 710 707 Size: 3 3728 BseYI C'CCAG_C Cuts at: 331 1156 1914 2491 2987 331 Size: 825 758 577 496 1075 Fragments arranged by size: 1075 825 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1273 1319 2527 104 Size: 1169 46 1208 1308 Fragments arranged by size: 1308 1208 1169 46 BsiHKAI G_wGCw'C Cuts at: 351 745 1523 1713 2505 351 Size: 394 778 190 792 1577 Fragments arranged by size: 1577 792 778 394 190 BsmI GAATG_Cn' Cuts at: 534 534 Size: 3731 Bsp1286I G_dGCh'C Cuts at: 351 745 1356 1449 1523 1713 1775 2505 351 Size: 394 611 93 74 190 62 730 1577 Fragments arranged by size: 1577 730 611 394 190 93 74 62 BspEI T'CCGG_A Cuts at: 2951 2951 Size: 3731 BspHI T'CATG_A Cuts at: 2907 2907 Size: 3731 BspMI ACCTGCnnnn'nnnn_ Cuts at: 781 995 1297 1678 781 Size: 214 302 381 2834 Fragments arranged by size: 2834 381 302 214 BsrBI CCG'CTC Cuts at: 50 2023 2077 3561 3726 50 Size: 1973 54 1484 165 55 Fragments arranged by size: 1973 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1643 1643 Size: 3731 BsrFI r'CCGG_y Cuts at: 1729 1910 2119 1729 Size: 181 209 3341 Fragments arranged by size: 3341 209 181 BsrGI T'GTAC_A Cuts at: 436 579 436 Size: 143 3588 BssSI C'ACGA_G Cuts at: 2002 2360 2002 Size: 358 3373 BstAPI GCAn_nnn'nTGC Cuts at: 1108 1108 Size: 3731 BstBI TT'CG_AA Cuts at: 2092 2092 Size: 3731 BstYI r'GATC_y Cuts at: 1 137 756 1223 1246 1581 1827 2839 2925 Size: 136 619 467 23 335 246 1012 86 Cuts at: 2925 2937 3283 1 Size: 12 346 449 Fragments arranged by size: 1012 619 467 449 346 335 246 136 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 3731 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1681 1862 2025 1681 Size: 181 163 3387 Fragments arranged by size: 3387 181 163 BtsI GCAGTG_nn' Cuts at: 974 974 Size: 3731 ClaI AT'CG_AT Cuts at: 479 479 Size: 3731 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 242 277 242 Size: 35 3696 DraI TTT'AAA Cuts at: 730 730 Size: 3731 DraIII CAC_nnn'GTG Cuts at: 355 355 Size: 3731 DrdI GACnn_nn'nnGTC Cuts at: 1437 2295 1437 Size: 858 2873 EaeI y'GGCC_r Cuts at: 1316 1490 1881 1908 3253 1316 Size: 174 391 27 1345 1794 Fragments arranged by size: 1794 1345 391 174 27 EagI C'GGCC_G Cuts at: 1316 1316 Size: 3731 EarI CTCTTCn'nnn_ Cuts at: 378 1754 1964 378 Size: 1376 210 2145 Fragments arranged by size: 2145 1376 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1939 2249 2395 3541 1939 Size: 310 146 1146 2129 Fragments arranged by size: 2129 1146 310 146 EcoICRI GAG'CTC Cuts at: 743 743 Size: 3731 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 154 1556 1988 2735 3003 154 Size: 1402 432 747 268 882 Fragments arranged by size: 1402 882 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 870 870 Size: 3731 EcoRI G'AATT_C Cuts at: 735 735 Size: 3731 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1750 1750 Size: 3731 FspI TGC'GCA Cuts at: 1512 1512 Size: 3731 HaeII r_GCGC'y Cuts at: 1165 1413 2435 3414 1165 Size: 248 1022 979 1482 Fragments arranged by size: 1482 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1573 1603 1605 1635 1573 Size: 30 2 30 3669 Fragments arranged by size: 3669 30 30 2 HincII GTy'rAC Cuts at: 237 770 237 Size: 533 3198 KasI G'GCGC_C Cuts at: 1161 1409 1161 Size: 248 3483 KpnI G_GTAC'C Cuts at: 751 751 Size: 3731 MluI A'CGCG_T Cuts at: 3164 3164 Size: 3731 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 447 2402 2586 447 Size: 1955 184 1592 Fragments arranged by size: 1955 1592 184 MscI TGG'CCA Cuts at: 1492 1492 Size: 3731 MslI CAynn'nnrTG Cuts at: 1720 1847 1720 Size: 127 3604 MspA1I CmG'CkG Cuts at: 842 1156 1516 2529 2774 842 Size: 314 360 1013 245 1799 Fragments arranged by size: 1799 1013 360 314 245 NaeI GCC'GGC Cuts at: 1912 1912 Size: 3731 NarI GG'CG_CC Cuts at: 1162 1410 1162 Size: 248 3483 NcoI C'CATG_G Cuts at: 107 107 Size: 3731 NgoMIV G'CCGG_C Cuts at: 1910 1910 Size: 3731 NspI r_CATG'y Cuts at: 462 784 1284 2191 462 Size: 322 500 907 2002 Fragments arranged by size: 2002 907 500 322 PciI A'CATG_T Cuts at: 2187 2187 Size: 3731 PmeI GTTT'AAAC Cuts at: 730 730 Size: 3731 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 312 344 2870 2902 312 Size: 32 2526 32 1141 Fragments arranged by size: 2526 1141 32 32 PstI C_TGCA'G Cuts at: 778 1463 778 Size: 685 3046 PvuI CG_AT'CG Cuts at: 104 104 Size: 3731 PvuII CAG'CTG Cuts at: 1156 1516 1156 Size: 360 3371 RsrII CG'GwC_CG Cuts at: 1926 1926 Size: 3731 SacI G_AGCT'C Cuts at: 745 745 Size: 3731 SalI G'TCGA_C Cuts at: 768 768 Size: 3731 SapI GCTCTTCn'nnn_ Cuts at: 1754 1964 1754 Size: 210 3521 SbfI CC_TGCA'GG Cuts at: 778 778 Size: 3731 ScaI AGT'ACT Cuts at: 3248 3248 Size: 3731 SfcI C'TryA_G Cuts at: 33 774 1459 2452 2643 33 Size: 741 685 993 191 1121 Fragments arranged by size: 1121 993 741 685 191 SfoI GGC'GCC Cuts at: 1163 1411 1163 Size: 248 3483 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3731 SmaI CCC'GGG Cuts at: 753 753 Size: 3731 SmlI C'TyrA_G Cuts at: 183 886 1801 2293 2555 183 Size: 703 915 492 262 1359 Fragments arranged by size: 1359 915 703 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3731 SphI G_CATG'C Cuts at: 784 1284 784 Size: 500 3231 StuI AGG'CCT Cuts at: 515 515 Size: 3731 StyI C'CwwG_G Cuts at: 107 687 865 1219 107 Size: 580 178 354 2619 Fragments arranged by size: 2619 580 354 178 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1314 1447 1906 2115 1314 Size: 133 459 209 2930 Fragments arranged by size: 2930 459 209 133 TatI w'GTAC_w Cuts at: 436 579 3246 436 Size: 143 2667 921 Fragments arranged by size: 2667 921 143 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3248 3280 3248 Size: 32 3699 Tth111I GACn'n_nGTC Cuts at: 1528 1528 Size: 3731 XbaI T'CTAG_A Cuts at: 762 762 Size: 3731 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1226 1226 Size: 3731 XmaI C'CCGG_G Cuts at: 751 751 Size: 3731 XmnI GAAnn'nnTTC Cuts at: 534 534 Size: 3731 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI ApaI AscI AvrII BbsI BbvCI BcgI BfrBI BglI BmtI BpmI Bpu10I BsgI BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI