Restriction map for pF1KB8323
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaHI       BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DdeI        DpnII       DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRII      
EcoRV       EcoT14I     FauI        FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HpaI        KasI        KpnI        KspI        
MaeI        MboI        MfeI        MflI        MluI        
MroI        MslI        MunI        MvaI        MvnI        
NaeI        NarI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RsrII       
SacII       SalI        Sau3AI      SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AciI           16  CCGC                 CCGC(-3/-1)
AfaI          309  GTAC                 GT^AC
AflIII        523  ACACGT               A^C[GA][CT]GT
AlwNI         445  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI          58  GAGCTC               G[AT]GC[AT]^C
AvaI          211  CCCGGG               C^[CT]CG[GA]G
AvaII         215  GGACC                G^G[AT]CC
BalI          602  TGGCCA               TGG^CCA
BbvI          573  GCTGC                GCAGC(8/12)
BsaAI         524  CACGTA               [CT]AC^GT[GA]
BsaBI         454  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          427  GGTCTC               GGTCTC(1/5)
BsaJI         211  CCCGGG               C^C[ACGT][ACGT]GG
BsiHKAI        58  GAGCTC               G[AT]GC[AT]^C
BsiYI          16  CCGCTGAATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           16  CCGCTGAATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         428  GTCTC                GTCTC(1/5)
BsoBI         211  CCCGGG               C^[CT]CG[GA]G
BspHI         125  TCATGA               T^CATGA
BsrDI         542  GCAATG               GCAATG(2/0)
Cfr13I        215  GGACC                G^G[ACGT]CC
DraII         214  GGGACCC              [GA]G^G[ACGT]CC[CT]
EaeI          602  TGGCCA               [CT]^GGCC[GA]
EcoO109I      214  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoT22I       668  ATGCAT               ATGCA^T
HapII         212  CCGG                 C^CGG
HincII        169  GTTGAC               GT[CT]^[GA]AC
HindII        169  GTTGAC               GT[CT]^[GA]AC
HindIII       316  AAGCTT               A^AGCTT
HpaII         212  CCGG                 C^CGG
MaeII         525  ACGT                 A^CGT
MaeIII        160  GTGAC                ^GT[ACGT]AC
MamI          454  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         602  TGGCCA               TGG^CCA
MscI          602  TGGCCA               TGG^CCA
MseI          313  TTAA                 T^TAA
MspA1I         16  CCGCTG               C[AC]G^C[GT]G
MspI          212  CCGG                 C^CGG
NlaIII        126  CATG                 CATG^
NsiI          668  ATGCAT               ATGCA^T
PleI          749  GACTC                GAGTC(4/5)
PpuMI         214  GGGACCC              [GA]G^G[AT]CC[CT]
RcaI          125  TCATGA               T^CATGA
RsaI          309  GTAC                 GT^AC
SacI           58  GAGCTC               GAGCT^C
Sau96I        215  GGACC                G^G[ACGT]CC
ScaI          308  AGTACT               AGT^ACT
SmaI          211  CCCGGG               CCC^GGG
Tru9I         313  TTAA                 T^TAA
TseI          573  GCTGC                G^C[AT]GC
Tsp45I        160  GTGAC                ^GT[GC]AC
XmaCI         211  CCCGGG               C^CCGGG
XmaI          211  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

BanII          46  GAGCCC               G[GA]GC[CT]^C
               58  GAGCTC              
BcnI          211  CCCGG                CC^[GC]GG
              212  CCGGG               
BmyI           46  GAGCCC               G[AGT]GC[ACT]^C
               58  GAGCTC              
BpmI          418  CTGGAG               CTGGAG(16/14)
              651  CTGGAG              
BseRI         470  CTCCTC               GAGGAG(10/8)
               34  GAGGAG              
Bsp1286I       46  GAGCCC               G[AGT]GC[ACT]^C
               58  GAGCTC              
BspMI         194  ACCTGC               ACCTGC(4/8)
              569  ACCTGC              
BssKI         211  CCCGG                ^CC[ACGT]GG
              212  CCGGG               
Cac8I          73  GCTGGC               GC[ACGT]^[ACGT]GC
              186  GCCAGC              
EcoRI         390  GAATTC               G^AATTC
              465  GAATTC              
Fnu4HI         15  GCCGC                GC^[ACGT]GC
              573  GCTGC               
HaeIII        398  GGCC                 GG^CC
              603  GGCC                
ItaI           15  GCCGC                GC^[ACGT]GC
              573  GCTGC               
NciI          211  CCCGG                CC^[GC]GG
              212  CCGGG               
PstI          196  CTGCAG               CTGCA^G
              442  CTGCAG              
SapI           60  GCTCTTC              GCTCTTC(1/4)
              120  GCTCTTC             
ScrFI         211  CCCGG                CC^[ACGT]GG
              212  CCGGG               
SfaNI          12  GATGC                GCATC(5/9)
              647  GCATC               
SfcI          196  CTGCAG               C^T[GA][CT]AG
              442  CTGCAG              
TaqI           10  TCGA                 T^CGA
               56  TCGA                
TfiI          129  GATTC                G^A[AT]TC
              337  GAATC               
TthHB8I        10  TCGA                 T^CGA
               56  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AcsI        AluI        ApoI        BsmFI       BsrI        
BstF5I      EarI        FokI        HinfI       HphI        
Ksp632I     MboII       MnlI        MwoI        NlaIV       
Tsp509I     TspRI       
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