Restriction map for pF1KB8323
 (Linear) (Six-base) MAPSORT of: KIBB8323.seq  Check: 5934  from: 1  to: 759



KIBB8323.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     759 
   Size:         49     710
 
AflIII A'CryG_T

Cuts at:      0     523     759 
   Size:        523     236
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     589     759 
   Size:        589     170
 
AlwNI CAG_nnn'CTG

Cuts at:      0     450     759 
   Size:        450     309
 
ApoI r'AATT_y

Cuts at:      0     277     370     390     465     529     759 
   Size:        277      93      20      75      64     230

  Fragments arranged by size: 

                 277     230      93      75      64      20
 
AvaI C'yCGr_G

Cuts at:      0     211     759 
   Size:        211     548
 
BanII G_rGCy'C

Cuts at:      0      50      62     759 
   Size:         50      12     697

  Fragments arranged by size: 

                 697      50      12
 
BfrBI ATG'CAT

Cuts at:      0     670     759 
   Size:        670      89
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     698     759 
   Size:        698      61
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     439     672     759 
   Size:        439     233      87

  Fragments arranged by size: 

                 439     233      87
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     433     759 
   Size:        433     326
 
BsaAI yAC'GTr

Cuts at:      0     526     759 
   Size:        526     233
 
BsaBI GATnn'nnATC

Cuts at:      0     458     759 
   Size:        458     301
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      49     461     759 
   Size:         49     412     298

  Fragments arranged by size: 

                 412     298      49
 
BsiHKAI G_wGCw'C

Cuts at:      0      62     759 
   Size:         62     697
 
Bsp1286I G_dGCh'C

Cuts at:      0      50      62     759 
   Size:         50      12     697

  Fragments arranged by size: 

                 697      50      12
 
BspHI T'CATG_A

Cuts at:      0     125     759 
   Size:        125     634
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     203     578     759 
   Size:        203     375     181

  Fragments arranged by size: 

                 375     203     181
 
BsrDI GCAATG_nn'

Cuts at:      0     549     759 
   Size:        549     210
 
BtsI GCAGTG_nn'

Cuts at:      0      84     759 
   Size:         84     675
 
EaeI y'GGCC_r

Cuts at:      0     602     759 
   Size:        602     157
 
EarI CTCTTCn'nnn_

Cuts at:      0      67     127     281     759 
   Size:         67      60     154     478

  Fragments arranged by size: 

                 478     154      67      60
 
EcoICRI GAG'CTC

Cuts at:      0      60     759 
   Size:         60     699
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     439     672     759 
   Size:         49     390     233      87

  Fragments arranged by size: 

                 390     233      87      49
 
EcoO109I rG'GnC_Cy

Cuts at:      0     215     759 
   Size:        215     544
 
EcoRI G'AATT_C

Cuts at:      0     390     465     759 
   Size:        390      75     294

  Fragments arranged by size: 

                 390     294      75
 
HincII GTy'rAC

Cuts at:      0     171     759 
   Size:        171     588
 
HindIII A'AGCT_T

Cuts at:      0     316     759 
   Size:        316     443
 
MscI TGG'CCA

Cuts at:      0     604     759 
   Size:        604     155
 
MspA1I CmG'CkG

Cuts at:      0      18     759 
   Size:         18     741
 
NsiI A_TGCA'T

Cuts at:      0     672     759 
   Size:        672      87
 
PpuMI rG'GwC_Cy

Cuts at:      0     215     759 
   Size:        215     544
 
PsiI TTA'TAA

Cuts at:      0     232     759 
   Size:        232     527
 
PstI C_TGCA'G

Cuts at:      0     200     446     759 
   Size:        200     246     313

  Fragments arranged by size: 

                 313     246     200
 
SacI G_AGCT'C

Cuts at:      0      62     759 
   Size:         62     697
 
SanDI GG'GwC_CC

Cuts at:      0     215     759 
   Size:        215     544
 
SapI GCTCTTCn'nnn_

Cuts at:      0      67     127     759 
   Size:         67      60     632

  Fragments arranged by size: 

                 632      67      60
 
ScaI AGT'ACT

Cuts at:      0     310     759 
   Size:        310     449
 
SfcI C'TryA_G

Cuts at:      0     196     442     759 
   Size:        196     246     317

  Fragments arranged by size: 

                 317     246     196
 
SmaI CCC'GGG

Cuts at:      0     213     759 
   Size:        213     546
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     236     347     759 
   Size:        236     111     412

  Fragments arranged by size: 

                 412     236     111
 
TatI w'GTAC_w

Cuts at:      0     308     759 
   Size:        308     451
 
XmaI C'CCGG_G

Cuts at:      0     211     759 
   Size:        211     548
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI    AlwNI     ApoI     AvaI    BanII    BfrBI
     BmrI     BpmI     BsaI    BsaAI    BsaBI    BseRI  BsiHKAI Bsp1286I
    BspHI    BspMI    BsrDI     BtsI     EaeI     EarI  EcoICRI  Eco57MI
 EcoO109I    EcoRI   HincII  HindIII     MscI   MspA1I     NsiI    PpuMI
     PsiI     PstI     SacI    SanDI     SapI     ScaI     SfcI     SmaI
    TaqII     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I    BpuEI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII    SacII     SalI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com