Restriction map for pF1KB8317
 (Circular) (Six-base) MAPSORT of: pF1KB8317.seq  Check: 7408  from: 1  to: 4571



pF1KB8317  4571 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    720     720 
   Size:       4571
 
AccI GT'mk_AC

Cuts at:   1609    1609 
   Size:       4571
 
Acc65I G'GTAC_C

Cuts at:   1587    1587 
   Size:       4571
 
AclI AA'CG_TT

Cuts at:   4411    4411 
   Size:       4571
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    431    1135    2396    2828    3575    3843     431 
   Size:        704    1261     432     747     268    1159

  Fragments arranged by size: 

                1261    1159     747     704     432     268
 
AflII C'TTAA_G

Cuts at:    430     430 
   Size:       4571
 
AflIII A'CryG_T

Cuts at:   1923    3027    4004    1923 
   Size:       1104     977    2490

  Fragments arranged by size: 

                2490    1104     977
 
AgeI A'CCGG_T

Cuts at:   2959    2959 
   Size:       4571
 
AlwNI CAG_nnn'CTG

Cuts at:    658     943    1343    1876    3443     658 
   Size:        285     400     533    1567    1786

  Fragments arranged by size: 

                1786    1567     533     400     285
 
ApaLI G'TGCA_C

Cuts at:   3341    3341 
   Size:       4571
 
ApoI r'AATT_y

Cuts at:   1575    3924    4180    1575 
   Size:       2349     256    1966

  Fragments arranged by size: 

                2349    1966     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4571
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4571
 
AvaI C'yCGr_G

Cuts at:   1031    1285    1591    1031 
   Size:        254     306    4011

  Fragments arranged by size: 

                4011     306     254
 
BamHI G'GATC_C

Cuts at:   1596    1596 
   Size:       4571
 
BanI G'GyrC_C

Cuts at:    691    1587    2001    2249    2284     691 
   Size:        896     414     248      35    2978

  Fragments arranged by size: 

                2978     896     414     248      35
 
BanII G_rGCy'C

Cuts at:    131     242     275     601    1132    1286    1294    1322    1585 
   Size:        111      33     326     531     154       8      28     263

Cuts at:   1585    2615     131 
   Size:       1030    2087

  Fragments arranged by size: 

                2087    1030     531     326     263     154     111      33
                  28       8
 
BbeI G_GCGC'C

Cuts at:   2005    2253    2005 
   Size:        248    4323
 
BbsI GAAGACnn'nnnn_

Cuts at:    155     971    1123     155 
   Size:        816     152    3603

  Fragments arranged by size: 

                3603     816     152
 
BbvCI CC'TCA_GC

Cuts at:    809     878     809 
   Size:         69    4502
 
BciVI GTATCCnnnnn_n'

Cuts at:   2469    3230    4571    2469 
   Size:        761    1341    2469

  Fragments arranged by size: 

                2469    1341     761
 
BclI T'GATC_A

Cuts at:    479     848    2091     479 
   Size:        369    1243    2959

  Fragments arranged by size: 

                2959    1243     369
 
BglI GCCn_nnn'nGGC

Cuts at:    952    1441     952 
   Size:        489    4082
 
BglII A'GATC_T

Cuts at:   2086    4123    2086 
   Size:       2037    2534
 
BlpI GC'TnA_GC

Cuts at:    269    1683     269 
   Size:       1414    3157
 
Bme1580I G_kGCm'C

Cuts at:   2196    2289    3345    2196 
   Size:         93    1056    3422

  Fragments arranged by size: 

                3422    1056      93
 
BmgBI CAC'GTC

Cuts at:    716     716 
   Size:       4571
 
BmrI ACTGGGnnnn_n'

Cuts at:    596    1783    1864    2199    4343     596 
   Size:       1187      81     335    2144     824

  Fragments arranged by size: 

                2144    1187     824     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2938    2938 
   Size:       4571
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    145     635     766    1399     145 
   Size:        490     131     633    3317

  Fragments arranged by size: 

                3317     633     490     131
 
Bpu10I CC'TnA_GC

Cuts at:    130     809     878    1280    1316     130 
   Size:        679      69     402      36    3385

  Fragments arranged by size: 

                3385     679     402      69      36
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    154     169    1350    1747    2626    3118    3416     154 
   Size:         15    1181     397     879     492     298    1309

  Fragments arranged by size: 

                1309    1181     879     492     397     298      15
 
BsaI GGTCTCn'nnnn_

Cuts at:    693     994    4275     693 
   Size:        301    3281     989

  Fragments arranged by size: 

                3281     989     301
 
BsaAI yAC'GTr

Cuts at:   1924    1924 
   Size:       4571
 
BsaBI GATnn'nnATC

Cuts at:      6    2085    4122       6 
   Size:       2079    2037     455

  Fragments arranged by size: 

                2079    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    675    1543    2002    2250    2952    4448     675 
   Size:        868     459     248     702    1496     798

  Fragments arranged by size: 

                1496     868     798     702     459     248
 
BsaWI w'CCGG_w

Cuts at:   1760    2281    2959    3233    3380    3791    1760 
   Size:        521     678     274     147     411    2540

  Fragments arranged by size: 

                2540     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1173    1366    1382    1537    1173 
   Size:        193      16     155    4207

  Fragments arranged by size: 

                4207     193     155      16
 
BseYI C'CCAG_C

Cuts at:    115     457    1996    2754    3331    3827     115 
   Size:        342    1539     758     577     496     859

  Fragments arranged by size: 

                1539     859     758     577     496     342
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    265     748    1015     265 
   Size:        483     267    3821

  Fragments arranged by size: 

                3821     483     267
 
BsiEI CG_ry'CG

Cuts at:    104    1445    2113    2159    3367     104 
   Size:       1341     668      46    1208    1308

  Fragments arranged by size: 

                1341    1308    1208     668      46
 
BsiHKAI G_wGCw'C

Cuts at:   1205    1294    1585    2363    2553    3345    1205 
   Size:         89     291     778     190     792    2431

  Fragments arranged by size: 

                2431     792     778     291     190      89
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1030    1030 
   Size:       4571
 
Bsp1286I G_dGCh'C

Cuts at:    131     242     275     601    1132    1205    1286    1294    1322 
   Size:        111      33     326     531      73      81       8      28

Cuts at:   1322    1585    2196    2289    2363    2553    2615    3345     131 
   Size:        263     611      93      74     190      62     730    1357

  Fragments arranged by size: 

                1357     730     611     531     326     263     190     111
                  93      81      74      73      62      33      28       8
            
 
BspEI T'CCGG_A

Cuts at:   3791    3791 
   Size:       4571
 
BspHI T'CATG_A

Cuts at:   3747    3747 
   Size:       4571
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    720    1621    1835    2137    2518     720 
   Size:        901     214     302     381    2773

  Fragments arranged by size: 

                2773     901     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     452     689    1103    2863    2917    4401    4566      50 
   Size:        402     237     414    1760      54    1484     165      55

  Fragments arranged by size: 

                1760    1484     414     402     237     165      55      54
            
 
BsrDI GCAATG_nn'

Cuts at:   2483    2483 
   Size:       4571
 
BsrFI r'CCGG_y

Cuts at:   2569    2750    2959    2569 
   Size:        181     209    4181

  Fragments arranged by size: 

                4181     209     181
 
BssSI C'ACGA_G

Cuts at:    696    2842    3200     696 
   Size:       2146     358    2067

  Fragments arranged by size: 

                2146    2067     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    943    1343    1948     943 
   Size:        400     605    3566

  Fragments arranged by size: 

                3566     605     400
 
BstBI TT'CG_AA

Cuts at:    533    2932     533 
   Size:       2399    2172
 
BstYI r'GATC_y

Cuts at:      1    1596    2063    2086    2421    2667    3679    3765    3777 
   Size:       1595     467      23     335     246    1012      86      12

Cuts at:   3777    4123       1 
   Size:        346     449

  Fragments arranged by size: 

                1595    1012     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    890     890 
   Size:       4571
 
BtgI C'CryG_G

Cuts at:    107    1421    1439     107 
   Size:       1314      18    3239

  Fragments arranged by size: 

                3239    1314      18
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2521    2702    2865    2521 
   Size:        181     163    4227

  Fragments arranged by size: 

                4227     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1249    1814    1249 
   Size:        565    4006
 
DraI TTT'AAA

Cuts at:    192    1570     192 
   Size:       1378    3193
 
DrdI GACnn_nn'nnGTC

Cuts at:   2277    3135    2277 
   Size:        858    3713
 
EaeI y'GGCC_r

Cuts at:    447     710    1442    2156    2330    2721    2748    4093     447 
   Size:        263     732     714     174     391      27    1345     925

  Fragments arranged by size: 

                1345     925     732     714     391     263     174      27
            
 
EagI C'GGCC_G

Cuts at:   1442    2156    1442 
   Size:        714    3857
 
EarI CTCTTCn'nnn_

Cuts at:    212    1093    2594    2804     212 
   Size:        881    1501     210    1979

  Fragments arranged by size: 

                1979    1501     881     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    306    1152    2779    3089    3235    4381     306 
   Size:        846    1627     310     146    1146     496

  Fragments arranged by size: 

                1627    1146     846     496     310     146
 
EcoICRI GAG'CTC

Cuts at:   1292    1583    1292 
   Size:        291    4280
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    145     431     635     766    1135    1399    2396    2828    3575 
   Size:        286     204     131     369     264     997     432     747

Cuts at:   3575    3843     145 
   Size:        268     873

  Fragments arranged by size: 

                 997     873     747     432     369     286     268     264
                 204     131
 
EcoO109I rG'GnC_Cy

Cuts at:    156     165     382     741    1239    1299    1710     156 
   Size:          9     217     359     498      60     411    3017

  Fragments arranged by size: 

                3017     498     411     359     217      60       9
 
EcoRI G'AATT_C

Cuts at:   1575    1575 
   Size:       4571
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2590    2590 
   Size:       4571
 
FspI TGC'GCA

Cuts at:   2352    2352 
   Size:       4571
 
HaeII r_GCGC'y

Cuts at:    363    1181    2005    2253    3275    4254     363 
   Size:        818     824     248    1022     979     680

  Fragments arranged by size: 

                1022     979     824     818     680     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2413    2443    2445    2475    2413 
   Size:         30       2      30    4509

  Fragments arranged by size: 

                4509      30      30       2
 
HincII GTy'rAC

Cuts at:   1610    1610 
   Size:       4571
 
HindIII A'AGCT_T

Cuts at:    335     427     335 
   Size:         92    4479
 
KasI G'GCGC_C

Cuts at:   2001    2249    2001 
   Size:        248    4323
 
KpnI G_GTAC'C

Cuts at:   1591    1591 
   Size:       4571
 
MluI A'CGCG_T

Cuts at:   4004    4004 
   Size:       4571
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3242    3426    3242 
   Size:        184    4387
 
MscI TGG'CCA

Cuts at:   2332    2332 
   Size:       4571
 
MslI CAynn'nnrTG

Cuts at:   2560    2687    2560 
   Size:        127    4444
 
MspA1I CmG'CkG

Cuts at:    357     943    1423    1682    1996    2356    3369    3614     357 
   Size:        586     480     259     314     360    1013     245    1314

  Fragments arranged by size: 

                1314    1013     586     480     360     314     259     245
            
 
NaeI GCC'GGC

Cuts at:   2752    2752 
   Size:       4571
 
NarI GG'CG_CC

Cuts at:   2002    2250    2002 
   Size:        248    4323
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4571
 
NgoMIV G'CCGG_C

Cuts at:   2750    2750 
   Size:       4571
 
NspI r_CATG'y

Cuts at:   1624    2124    3031    1624 
   Size:        500     907    3164

  Fragments arranged by size: 

                3164     907     500
 
PasI CC'CwG_GG

Cuts at:    489    1132     489 
   Size:        643    3928
 
PciI A'CATG_T

Cuts at:   3027    3027 
   Size:       4571
 
PflMI CCAn_nnn'nTGG

Cuts at:    658     843     658 
   Size:        185    4386
 
PfoI T'CCnGG_A

Cuts at:    651     743     651 
   Size:         92    4479
 
PmeI GTTT'AAAC

Cuts at:   1570    1570 
   Size:       4571
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3710    3742    3710 
   Size:         32    4539
 
PpuMI rG'GwC_Cy

Cuts at:    156     382     741    1299     156 
   Size:        226     359     558    3428

  Fragments arranged by size: 

                3428     558     359     226
 
PstI C_TGCA'G

Cuts at:   1618    2303    1618 
   Size:        685    3886
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4571
 
PvuII CAG'CTG

Cuts at:   1996    2356    1996 
   Size:        360    4211
 
RsrII CG'GwC_CG

Cuts at:   2766    2766 
   Size:       4571
 
SacI G_AGCT'C

Cuts at:   1294    1585    1294 
   Size:        291    4280
 
SacII CC_GC'GG

Cuts at:   1424    1424 
   Size:       4571
 
SalI G'TCGA_C

Cuts at:   1608    1608 
   Size:       4571
 
SanDI GG'GwC_CC

Cuts at:    382     382 
   Size:       4571
 
SapI GCTCTTCn'nnn_

Cuts at:   1093    2594    2804    1093 
   Size:       1501     210    2860

  Fragments arranged by size: 

                2860    1501     210
 
SbfI CC_TGCA'GG

Cuts at:   1618    1618 
   Size:       4571
 
ScaI AGT'ACT

Cuts at:   4088    4088 
   Size:       4571
 
SfcI C'TryA_G

Cuts at:     33    1614    2299    3292    3483      33 
   Size:       1581     685     993     191    1121

  Fragments arranged by size: 

                1581    1121     993     685     191
 
SfoI GGC'GCC

Cuts at:   2003    2251    2003 
   Size:        248    4323
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4571
 
SmaI CCC'GGG

Cuts at:   1033    1593    1033 
   Size:        560    4011
 
SmlI C'TyrA_G

Cuts at:    169     184     430    1329    1726    2641    3133    3395     169 
   Size:         15     246     899     397     915     492     262    1345

  Fragments arranged by size: 

                1345     915     899     492     397     262     246      15
            
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4571
 
SphI G_CATG'C

Cuts at:   1624    2124    1624 
   Size:        500    4071
 
StyI C'CwwG_G

Cuts at:    107     704    1160    1397    1507    1705    2059     107 
   Size:        597     456     237     110     198     354    2619

  Fragments arranged by size: 

                2619     597     456     354     237     198     110
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    850    1567    2154    2287    2746    2955     850 
   Size:        717     587     133     459     209    2466

  Fragments arranged by size: 

                2466     717     587     459     209     133
 
TatI w'GTAC_w

Cuts at:   1454    4086    1454 
   Size:       2632    1939
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1213    1245    4088    4120    1213 
   Size:         32    2843      32    1664

  Fragments arranged by size: 

                2843    1664      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2368    2368 
   Size:       4571
 
XbaI T'CTAG_A

Cuts at:   1602    1602 
   Size:       4571
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    840    2066     840 
   Size:       1226    3345
 
XmaI C'CCGG_G

Cuts at:   1031    1591    1031 
   Size:        560    4011
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI     AhdI     AleI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI     BcgI    BfrBI     BmtI    BsaXI    BsiWI     BsmI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I     ClaI    CspCI   DraIII
    EcoNI    EcoRV     FseI    FspAI     HpaI     MfeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     XmnI     ZraI
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