################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HapII HhaI HinP1I HincII HindII HindIII HpaI HpaII KasI KspI MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 18 GGTACC G^GTACC AciI 139 CCGC CCGC(-3/-1) AcsI 316 GAATTC [GA]^AATT[CT] AcyI 61 GACGCC G[GA]^CG[CT]C AlwNI 130 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 316 GAATTC [GA]^AATT[CT] Asp718I 18 GGTACC G^GTACC AspHI 253 GAGCTC G[AT]GC[AT]^C BanI 18 GGTACC G^G[CT][GA]CC BbsI 85 GAAGAC GAAGAC(2/6) BclI 2 TGATCA T^GATCA BlpI 301 GCTGAGC GC^T[ACGT]AGC BpmI 382 CTGGAG CTGGAG(16/14) Bpu1102I 301 GCTGAGC GC^T[ACGT]AGC BpuAI 85 GAAGAC GAAGAC(2/6) BsaHI 61 GACGCC G[GA]^CG[CT]C BsiHKAI 253 GAGCTC G[AT]GC[AT]^C BsmAI 363 GTCTC GTCTC(1/5) BspHI 147 TCATGA T^CATGA BspMI 21 ACCTGC ACCTGC(4/8) BsrDI 258 CATTGC GCAATG(2/0) BssKI 14 CCAGG ^CC[ACGT]GG BstBI 392 TTCGAA TT^CGAA BstF5I 294 GGATG GGATG(2/0) BstNI 14 CCAGG CC^[AT]GG BstXI 92 CCATTCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 301 GCTGAGC GC^T[ACGT]AGC Cfr13I 357 GGCCC G^G[ACGT]CC DsaI 164 CCATGG C^C[GA][CT]GG EcoRI 316 GAATTC G^AATTC EcoRII 14 CCAGG ^CC[AT]GG EcoT14I 164 CCATGG C^C[AT][AT]GG FbaI 2 TGATCA T^GATCA FokI 294 GGATG GGATG(9/13) HaeIII 357 GGCC GG^CC HgaI 61 GACGC GACGC(5/10) Hin1I 61 GACGCC G[GA]^CG[CT]C HphI 227 TCACC GGTGA(8/7) KpnI 18 GGTACC GGTAC^C MaeII 346 ACGT A^CGT MslI 165 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 14 CCAGG CC^[AT]GG NcoI 164 CCATGG C^CATGG NlaIV 18 GGTACC GG[ACGT]^[ACGT]CC NspV 392 TTCGAA TT^CGAA RcaI 147 TCATGA T^CATGA SacI 253 GAGCTC GAGCT^C Sau96I 357 GGCCC G^G[ACGT]CC ScrFI 14 CCAGG CC^[ACGT]GG SfcI 127 CTACAG C^T[GA][CT]AG SfuI 392 TTCGAA TT^CGAA StyI 164 CCATGG C^C[AT][AT]GG TspRI 184 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 19 GTAC GT^AC 37 GTAC BanII 253 GAGCTC G[GA]GC[CT]^C 417 GGGCTC BbvI 123 GCAGC GCAGC(8/12) 298 GCTGC BmyI 253 GAGCTC G[AGT]GC[ACT]^C 417 GGGCTC BseRI 283 GAGGAG GAGGAG(10/8) 334 GAGGAG Bsp1286I 253 GAGCTC G[AGT]GC[ACT]^C 417 GGGCTC BsrI 381 ACTGG ACTGG(1/-1) 360 CCAGT DdeI 66 CTCAG C^T[ACGT]AG 302 CTGAG DpnII 3 GATC ^GATC 271 GATC EarI 280 GAAGAG CTCTTC(1/4) 408 GAAGAG Ksp632I 280 GAAGAG CTCTTC(1/4) 408 GAAGAG MboI 3 GATC ^GATC 271 GATC MwoI 123 GCAGCTACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 132 GCCCCTGCCGC NdeII 3 GATC ^GATC 271 GATC NlaIII 148 CATG CATG^ 165 CATG PleI 88 GACTC GAGTC(4/5) 151 GACTC RsaI 19 GTAC GT^AC 37 GTAC Sau3AI 3 GATC ^GATC 271 GATC SfaNI 295 GATGC GCATC(5/9) 41 GCATC SmlI 208 CTTGAG C^T[CT][GA]AG 235 CTTGAG TaqI 320 TCGA T^CGA 393 TCGA TfiI 160 GAATC G^A[AT]TC 339 GAATC TseI 123 GCAGC G^C[AT]GC 298 GCTGC Tsp509I 113 AATT ^AATT 317 AATT TthHB8I 320 TCGA T^CGA 393 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### AluI BsaJI Fnu4HI HinfI ItaI MboII MnlI