(Circular) (Six-base) MAPSORT of: pF1KB8305.seq Check: 4429 from: 1 to: 5084 pF1KB8305 5084 bp With 182 enzymes: SgfI * June 20, 2008 10:28 .. AatII G_ACGT'C Cuts at: 174 335 174 Size: 161 4923 AccI GT'mk_AC Cuts at: 2122 2122 Size: 5084 Acc65I G'GTAC_C Cuts at: 2100 2100 Size: 5084 AclI AA'CG_TT Cuts at: 4924 4924 Size: 5084 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2018 2909 3341 4088 4356 2018 Size: 891 432 747 268 2746 Fragments arranged by size: 2746 891 747 432 268 AflIII A'CryG_T Cuts at: 1040 1847 2436 3540 4517 1040 Size: 807 589 1104 977 1607 Fragments arranged by size: 1607 1104 977 807 589 AgeI A'CCGG_T Cuts at: 3472 3472 Size: 5084 AleI CACnn'nnGTG Cuts at: 1123 1870 1123 Size: 747 4337 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 782 1756 782 Size: 974 4110 AlwNI CAG_nnn'CTG Cuts at: 1620 2389 3956 1620 Size: 769 1567 2748 Fragments arranged by size: 2748 1567 769 ApaLI G'TGCA_C Cuts at: 1048 3854 1048 Size: 2806 2278 ApoI r'AATT_y Cuts at: 319 864 2088 4437 4693 319 Size: 545 1224 2349 256 710 Fragments arranged by size: 2349 1224 710 545 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5084 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5084 AvaI C'yCGr_G Cuts at: 673 2104 673 Size: 1431 3653 BamHI G'GATC_C Cuts at: 843 2109 843 Size: 1266 3818 BanI G'GyrC_C Cuts at: 689 1125 1199 1286 1312 2100 2514 2762 2797 Size: 436 74 87 26 788 414 248 35 Cuts at: 2797 689 Size: 2976 Fragments arranged by size: 2976 788 436 414 248 87 74 35 26 BanII G_rGCy'C Cuts at: 470 680 1549 1807 2098 3128 470 Size: 210 869 258 291 1030 2426 Fragments arranged by size: 2426 1030 869 291 258 210 BbeI G_GCGC'C Cuts at: 1203 1290 2518 2766 1203 Size: 87 1228 248 3521 Fragments arranged by size: 3521 1228 248 87 BbsI GAAGACnn'nnnn_ Cuts at: 1516 1516 Size: 5084 BbvCI CC'TCA_GC Cuts at: 1958 2039 1958 Size: 81 5003 BciVI GTATCCnnnnn_n' Cuts at: 2982 3743 5084 2982 Size: 761 1341 2982 Fragments arranged by size: 2982 1341 761 BclI T'GATC_A Cuts at: 1814 2604 1814 Size: 790 4294 BglII A'GATC_T Cuts at: 2599 4636 2599 Size: 2037 3047 BlpI GC'TnA_GC Cuts at: 255 2196 255 Size: 1941 3143 Bme1580I G_kGCm'C Cuts at: 945 1052 1130 1275 2709 2802 3858 945 Size: 107 78 145 1434 93 1056 2171 Fragments arranged by size: 2171 1434 1056 145 107 93 78 BmgBI CAC'GTC Cuts at: 1344 1357 1848 1344 Size: 13 491 4580 Fragments arranged by size: 4580 491 13 BmrI ACTGGGnnnn_n' Cuts at: 630 1262 2296 2377 2712 4856 630 Size: 632 1034 81 335 2144 858 Fragments arranged by size: 2144 1034 858 632 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3451 3451 Size: 5084 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 142 1116 1447 1642 142 Size: 974 331 195 3584 Fragments arranged by size: 3584 974 331 195 Bpu10I CC'TnA_GC Cuts at: 749 850 1144 1958 2039 749 Size: 101 294 814 81 3794 Fragments arranged by size: 3794 814 294 101 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 516 2260 3139 3631 3929 516 Size: 1744 879 492 298 1671 Fragments arranged by size: 1744 1671 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 187 814 2022 4788 187 Size: 627 1208 2766 483 Fragments arranged by size: 2766 1208 627 483 BsaAI yAC'GTr Cuts at: 1041 2437 1041 Size: 1396 3688 BsaBI GATnn'nnATC Cuts at: 6 2598 4635 6 Size: 2592 2037 455 Fragments arranged by size: 2592 2037 455 BsaHI Gr'CG_yC Cuts at: 171 332 1098 1200 1287 2515 2763 3465 4961 Size: 161 766 102 87 1228 248 702 1496 Cuts at: 4961 171 Size: 294 Fragments arranged by size: 1496 1228 766 702 294 248 161 102 87 BsaWI w'CCGG_w Cuts at: 176 920 2273 2794 3472 3746 3893 4304 176 Size: 744 1353 521 678 274 147 411 956 Fragments arranged by size: 1353 956 744 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1248 1278 1615 1645 1248 Size: 30 337 30 4687 Fragments arranged by size: 4687 337 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 2056 2056 Size: 5084 BseYI C'CCAG_C Cuts at: 1699 1713 1943 2509 3267 3844 4340 1699 Size: 14 230 566 758 577 496 2443 Fragments arranged by size: 2443 758 577 566 496 230 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 317 878 1258 1495 1624 1848 317 Size: 561 380 237 129 224 3553 Fragments arranged by size: 3553 561 380 237 224 129 BsiEI CG_ry'CG Cuts at: 104 2626 2672 3880 104 Size: 2522 46 1208 1308 Fragments arranged by size: 2522 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 353 413 470 1052 1150 1425 1581 2098 2876 Size: 60 57 582 98 275 156 517 778 Cuts at: 2876 3066 3858 353 Size: 190 792 1579 Fragments arranged by size: 1579 792 778 582 517 275 190 156 98 60 57 BsmI GAATG_Cn' Cuts at: 1230 1230 Size: 5084 Bsp1286I G_dGCh'C Cuts at: 353 413 470 680 945 1052 1130 1150 1275 Size: 60 57 210 265 107 78 20 125 Cuts at: 1275 1425 1549 1581 1807 2098 2709 2802 2876 Size: 150 124 32 226 291 611 93 74 Cuts at: 2876 3066 3128 3858 353 Size: 190 62 730 1579 Fragments arranged by size: 1579 730 611 291 265 226 210 190 150 125 124 107 93 78 74 62 60 57 32 20 BspEI T'CCGG_A Cuts at: 920 4304 920 Size: 3384 1700 BspHI T'CATG_A Cuts at: 137 4260 137 Size: 4123 961 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1365 1646 2134 2348 2650 3031 1365 Size: 281 488 214 302 381 3418 Fragments arranged by size: 3418 488 381 302 281 214 BsrBI CCG'CTC Cuts at: 50 753 1139 3376 3430 4914 5079 50 Size: 703 386 2237 54 1484 165 55 Fragments arranged by size: 2237 1484 703 386 165 55 54 BsrDI GCAATG_nn' Cuts at: 764 2996 764 Size: 2232 2852 BsrFI r'CCGG_y Cuts at: 383 3082 3263 3472 383 Size: 2699 181 209 1995 Fragments arranged by size: 2699 1995 209 181 BssSI C'ACGA_G Cuts at: 3355 3713 3355 Size: 358 4726 BstAPI GCAn_nnn'nTGC Cuts at: 837 2461 837 Size: 1624 3460 BstBI TT'CG_AA Cuts at: 3445 3445 Size: 5084 BstXI CCAn_nnnn'nTGG Cuts at: 715 1527 715 Size: 812 4272 BstYI r'GATC_y Cuts at: 1 843 1436 1616 2109 2576 2599 2934 3180 Size: 842 593 180 493 467 23 335 246 Cuts at: 3180 4192 4278 4290 4636 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1012 842 593 493 467 449 346 335 246 180 86 23 12 Bsu36I CC'TnA_GG Cuts at: 547 1808 1999 547 Size: 1261 191 3632 Fragments arranged by size: 3632 1261 191 BtgI C'CryG_G Cuts at: 107 1194 1897 107 Size: 1087 703 3294 Fragments arranged by size: 3294 1087 703 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3034 3215 3378 3034 Size: 181 163 4740 Fragments arranged by size: 4740 181 163 BtsI GCAGTG_nn' Cuts at: 838 861 2327 838 Size: 23 1466 3595 Fragments arranged by size: 3595 1466 23 DraI TTT'AAA Cuts at: 2083 2083 Size: 5084 DraIII CAC_nnn'GTG Cuts at: 417 417 Size: 5084 DrdI GACnn_nn'nnGTC Cuts at: 1006 2790 3648 1006 Size: 1784 858 2442 Fragments arranged by size: 2442 1784 858 EaeI y'GGCC_r Cuts at: 717 1919 1986 2669 2843 3234 3261 4606 717 Size: 1202 67 683 174 391 27 1345 1195 Fragments arranged by size: 1345 1202 1195 683 391 174 67 27 EagI C'GGCC_G Cuts at: 2669 2669 Size: 5084 EarI CTCTTCn'nnn_ Cuts at: 3107 3317 3107 Size: 210 4874 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3292 3602 3748 4894 3292 Size: 310 146 1146 3482 Fragments arranged by size: 3482 1146 310 146 EcoICRI GAG'CTC Cuts at: 468 2096 468 Size: 1628 3456 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 142 1116 1447 1642 2018 2909 3341 4088 4356 Size: 974 331 195 376 891 432 747 268 Cuts at: 4356 142 Size: 870 Fragments arranged by size: 974 891 870 747 432 376 331 268 195 EcoNI CCTnn'n_nnAGG Cuts at: 894 1306 1320 894 Size: 412 14 4658 Fragments arranged by size: 4658 412 14 EcoO109I rG'GnC_Cy Cuts at: 779 2223 779 Size: 1444 3640 EcoRI G'AATT_C Cuts at: 2088 2088 Size: 5084 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 514 3103 514 Size: 2589 2495 FspI TGC'GCA Cuts at: 2865 2865 Size: 5084 HaeII r_GCGC'y Cuts at: 1035 1203 1290 2518 2766 3788 4767 1035 Size: 168 87 1228 248 1022 979 1352 Fragments arranged by size: 1352 1228 1022 979 248 168 87 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 514 546 898 930 1615 1647 1667 1699 2926 Size: 32 352 32 685 32 20 32 1227 Cuts at: 2926 2956 2958 2988 514 Size: 30 2 30 2610 Fragments arranged by size: 2610 1227 685 352 32 32 32 32 30 30 20 2 HincII GTy'rAC Cuts at: 2123 2123 Size: 5084 KasI G'GCGC_C Cuts at: 1199 1286 2514 2762 1199 Size: 87 1228 248 3521 Fragments arranged by size: 3521 1228 248 87 KpnI G_GTAC'C Cuts at: 2104 2104 Size: 5084 MfeI C'AATT_G Cuts at: 509 509 Size: 5084 MluI A'CGCG_T Cuts at: 4517 4517 Size: 5084 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3755 3939 3755 Size: 184 4900 MscI TGG'CCA Cuts at: 2845 2845 Size: 5084 MslI CAynn'nnrTG Cuts at: 1123 1525 1870 2011 3073 3200 1123 Size: 402 345 141 1062 127 3007 Fragments arranged by size: 3007 1062 402 345 141 127 MspA1I CmG'CkG Cuts at: 1820 1962 2038 2195 2509 2869 3882 4127 1820 Size: 142 76 157 314 360 1013 245 2777 Fragments arranged by size: 2777 1013 360 314 245 157 142 76 NaeI GCC'GGC Cuts at: 385 3265 385 Size: 2880 2204 NarI GG'CG_CC Cuts at: 1200 1287 2515 2763 1200 Size: 87 1228 248 3521 Fragments arranged by size: 3521 1228 248 87 NcoI C'CATG_G Cuts at: 107 107 Size: 5084 NgoMIV G'CCGG_C Cuts at: 383 3263 383 Size: 2880 2204 NspI r_CATG'y Cuts at: 840 1539 1632 1773 2137 2637 3544 840 Size: 699 93 141 364 500 907 2380 Fragments arranged by size: 2380 907 699 500 364 141 93 PciI A'CATG_T Cuts at: 3540 3540 Size: 5084 PflMI CCAn_nnn'nTGG Cuts at: 891 1283 891 Size: 392 4692 PfoI T'CCnGG_A Cuts at: 1619 1619 Size: 5084 PmeI GTTT'AAAC Cuts at: 2083 2083 Size: 5084 PmlI CAC'GTG Cuts at: 1041 1041 Size: 5084 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 793 825 4223 4255 793 Size: 32 3398 32 1622 Fragments arranged by size: 3398 1622 32 32 PpuMI rG'GwC_Cy Cuts at: 779 779 Size: 5084 PshAI GACnn'nnGTC Cuts at: 215 215 Size: 5084 PstI C_TGCA'G Cuts at: 1745 2131 2816 1745 Size: 386 685 4013 Fragments arranged by size: 4013 685 386 PvuI CG_AT'CG Cuts at: 104 104 Size: 5084 PvuII CAG'CTG Cuts at: 1962 2038 2509 2869 1962 Size: 76 471 360 4177 Fragments arranged by size: 4177 471 360 76 RsrII CG'GwC_CG Cuts at: 3279 3279 Size: 5084 SacI G_AGCT'C Cuts at: 470 2098 470 Size: 1628 3456 SalI G'TCGA_C Cuts at: 2121 2121 Size: 5084 SapI GCTCTTCn'nnn_ Cuts at: 3107 3317 3107 Size: 210 4874 SbfI CC_TGCA'GG Cuts at: 2131 2131 Size: 5084 ScaI AGT'ACT Cuts at: 1480 4601 1480 Size: 3121 1963 SfcI C'TryA_G Cuts at: 33 1062 1741 2127 2812 3805 3996 33 Size: 1029 679 386 685 993 191 1121 Fragments arranged by size: 1121 1029 993 685 679 386 191 SfoI GGC'GCC Cuts at: 1201 1288 2516 2764 1201 Size: 87 1228 248 3521 Fragments arranged by size: 3521 1228 248 87 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5084 SmaI CCC'GGG Cuts at: 2106 2106 Size: 5084 SmlI C'TyrA_G Cuts at: 531 2239 3154 3646 3908 531 Size: 1708 915 492 262 1707 Fragments arranged by size: 1708 1707 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5084 SphI G_CATG'C Cuts at: 840 1773 2137 2637 840 Size: 933 364 500 3287 Fragments arranged by size: 3287 933 500 364 StyI C'CwwG_G Cuts at: 107 890 2218 2572 107 Size: 783 1328 354 2619 Fragments arranged by size: 2619 1328 783 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1957 2667 2800 3259 3468 1957 Size: 710 133 459 209 3573 Fragments arranged by size: 3573 710 459 209 133 TatI w'GTAC_w Cuts at: 1478 1532 1604 4599 1478 Size: 54 72 2995 1963 Fragments arranged by size: 2995 1963 72 54 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4601 4633 4601 Size: 32 5052 Tth111I GACn'n_nGTC Cuts at: 2881 2881 Size: 5084 XbaI T'CTAG_A Cuts at: 2115 2115 Size: 5084 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2579 2579 Size: 5084 XmaI C'CCGG_G Cuts at: 2104 2104 Size: 5084 ZraI GAC'GTC Cuts at: 172 333 172 Size: 161 4923 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AarI AfeI AflII AhdI AloI ApaI AscI AvrII BaeI BcgI BfrBI BglI BmtI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI NruI NsiI PacI PasI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI