Restriction map for pF1KB8305
 (Linear) (Six-base) MAPSORT of: KIBB8305.seq  Check: 6943  from: 1  to: 1971



KIBB8305.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AatII G_ACGT'C

Cuts at:      0      66     227    1971 
   Size:         66     161    1744

  Fragments arranged by size: 

                1744     161      66
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1910    1971 
   Size:       1910      61
 
AflIII A'CryG_T

Cuts at:      0     932    1739    1971 
   Size:        932     807     232

  Fragments arranged by size: 

                 932     807     232
 
AleI CACnn'nnGTG

Cuts at:      0    1015    1762    1971 
   Size:       1015     747     209

  Fragments arranged by size: 

                1015     747     209
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     674    1648    1971 
   Size:        674     974     323

  Fragments arranged by size: 

                 974     674     323
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1512    1971 
   Size:       1512     459
 
ApaLI G'TGCA_C

Cuts at:      0     940    1971 
   Size:        940    1031
 
ApoI r'AATT_y

Cuts at:      0     211     756    1971 
   Size:        211     545    1215

  Fragments arranged by size: 

                1215     545     211
 
AvaI C'yCGr_G

Cuts at:      0     565    1971 
   Size:        565    1406
 
BamHI G'GATC_C

Cuts at:      0     735    1971 
   Size:        735    1236
 
BanI G'GyrC_C

Cuts at:      0     581    1017    1091    1178    1204    1971 
   Size:        581     436      74      87      26     767

  Fragments arranged by size: 

                 767     581     436      87      74      26
 
BanII G_rGCy'C

Cuts at:      0     362     572    1441    1699    1971 
   Size:        362     210     869     258     272

  Fragments arranged by size: 

                 869     362     272     258     210
 
BbeI G_GCGC'C

Cuts at:      0    1095    1182    1971 
   Size:       1095      87     789

  Fragments arranged by size: 

                1095     789      87
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1408    1971 
   Size:       1408     563
 
BbvCI CC'TCA_GC

Cuts at:      0    1850    1931    1971 
   Size:       1850      81      40

  Fragments arranged by size: 

                1850      81      40
 
BclI T'GATC_A

Cuts at:      0    1706    1971 
   Size:       1706     265
 
BlpI GC'TnA_GC

Cuts at:      0     147    1971 
   Size:        147    1824
 
Bme1580I G_kGCm'C

Cuts at:      0     837     944    1022    1167    1971 
   Size:        837     107      78     145     804

  Fragments arranged by size: 

                 837     804     145     107      78
 
BmgBI CAC'GTC

Cuts at:      0    1236    1249    1740    1971 
   Size:       1236      13     491     231

  Fragments arranged by size: 

                1236     491     231      13
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     522    1154    1971 
   Size:        522     632     817

  Fragments arranged by size: 

                 817     632     522
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34    1008    1339    1534    1971 
   Size:         34     974     331     195     437

  Fragments arranged by size: 

                 974     437     331     195      34
 
Bpu10I CC'TnA_GC

Cuts at:      0     641     742    1036    1850    1931    1971 
   Size:        641     101     294     814      81      40

  Fragments arranged by size: 

                 814     641     294     101      81      40
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     408    1971 
   Size:        408    1563
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      79     706    1914    1971 
   Size:         79     627    1208      57

  Fragments arranged by size: 

                1208     627      79      57
 
BsaAI yAC'GTr

Cuts at:      0     933    1971 
   Size:        933    1038
 
BsaHI Gr'CG_yC

Cuts at:      0      63     224     990    1092    1179    1971 
   Size:         63     161     766     102      87     792

  Fragments arranged by size: 

                 792     766     161     102      87      63
 
BsaWI w'CCGG_w

Cuts at:      0      68     812    1971 
   Size:         68     744    1159

  Fragments arranged by size: 

                1159     744      68
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1140    1170    1507    1537    1971 
   Size:       1140      30     337      30     434

  Fragments arranged by size: 

                1140     434     337      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1948    1971 
   Size:       1948      23
 
BseYI C'CCAG_C

Cuts at:      0    1591    1605    1835    1971 
   Size:       1591      14     230     136

  Fragments arranged by size: 

                1591     230     136      14
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     770    1150    1387    1516    1740    1971 
   Size:        209     561     380     237     129     224     231

  Fragments arranged by size: 

                 561     380     237     231     224     209     129
 
BsiHKAI G_wGCw'C

Cuts at:      0     245     305     362     944    1042    1317    1473    1971 
   Size:        245      60      57     582      98     275     156     498

  Fragments arranged by size: 

                 582     498     275     245     156      98      60      57
            
 
BsmI GAATG_Cn'

Cuts at:      0    1122    1971 
   Size:       1122     849
 
Bsp1286I G_dGCh'C

Cuts at:      0     245     305     362     572     837     944    1022    1042 
   Size:        245      60      57     210     265     107      78      20

Cuts at:   1042    1167    1317    1441    1473    1699    1971 
   Size:        125     150     124      32     226     272

  Fragments arranged by size: 

                 272     265     245     226     210     150     125     124
                 107      78      60      57      32      20
 
BspEI T'CCGG_A

Cuts at:      0     812    1971 
   Size:        812    1159
 
BspHI T'CATG_A

Cuts at:      0      29    1971 
   Size:         29    1942
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1257    1538    1971 
   Size:       1257     281     433

  Fragments arranged by size: 

                1257     433     281
 
BsrBI CCG'CTC

Cuts at:      0     645    1031    1971 
   Size:        645     386     940

  Fragments arranged by size: 

                 940     645     386
 
BsrDI GCAATG_nn'

Cuts at:      0     656    1971 
   Size:        656    1315
 
BsrFI r'CCGG_y

Cuts at:      0     275    1971 
   Size:        275    1696
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     729    1971 
   Size:        729    1242
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     607    1419    1971 
   Size:        607     812     552

  Fragments arranged by size: 

                 812     607     552
 
BstYI r'GATC_y

Cuts at:      0     735    1328    1508    1971 
   Size:        735     593     180     463

  Fragments arranged by size: 

                 735     593     463     180
 
Bsu36I CC'TnA_GG

Cuts at:      0     439    1700    1891    1971 
   Size:        439    1261     191      80

  Fragments arranged by size: 

                1261     439     191      80
 
BtgI C'CryG_G

Cuts at:      0    1086    1789    1971 
   Size:       1086     703     182

  Fragments arranged by size: 

                1086     703     182
 
BtsI GCAGTG_nn'

Cuts at:      0     730     753    1971 
   Size:        730      23    1218

  Fragments arranged by size: 

                1218     730      23
 
DraIII CAC_nnn'GTG

Cuts at:      0     309    1971 
   Size:        309    1662
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     898    1971 
   Size:        898    1073
 
EaeI y'GGCC_r

Cuts at:      0     609    1811    1878    1971 
   Size:        609    1202      67      93

  Fragments arranged by size: 

                1202     609      93      67
 
EcoICRI GAG'CTC

Cuts at:      0     360    1971 
   Size:        360    1611
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34    1008    1339    1534    1910    1971 
   Size:         34     974     331     195     376      61

  Fragments arranged by size: 

                 974     376     331     195      61      34
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     786    1198    1212    1971 
   Size:        786     412      14     759

  Fragments arranged by size: 

                 786     759     412      14
 
EcoO109I rG'GnC_Cy

Cuts at:      0     671    1971 
   Size:        671    1300
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     406    1971 
   Size:        406    1565
 
HaeII r_GCGC'y

Cuts at:      0     927    1095    1182    1971 
   Size:        927     168      87     789

  Fragments arranged by size: 

                 927     789     168      87
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     406     438     790     822    1507    1539    1559    1591 
   Size:        406      32     352      32     685      32      20      32

Cuts at:   1591    1971 
   Size:        380

  Fragments arranged by size: 

                 685     406     380     352      32      32      32      32
                  20
 
KasI G'GCGC_C

Cuts at:      0    1091    1178    1971 
   Size:       1091      87     793

  Fragments arranged by size: 

                1091     793      87
 
MfeI C'AATT_G

Cuts at:      0     401    1971 
   Size:        401    1570
 
MslI CAynn'nnrTG

Cuts at:      0    1015    1417    1762    1903    1971 
   Size:       1015     402     345     141      68

  Fragments arranged by size: 

                1015     402     345     141      68
 
MspA1I CmG'CkG

Cuts at:      0    1712    1854    1930    1971 
   Size:       1712     142      76      41

  Fragments arranged by size: 

                1712     142      76      41
 
NaeI GCC'GGC

Cuts at:      0     277    1971 
   Size:        277    1694
 
NarI GG'CG_CC

Cuts at:      0    1092    1179    1971 
   Size:       1092      87     792

  Fragments arranged by size: 

                1092     792      87
 
NgoMIV G'CCGG_C

Cuts at:      0     275    1971 
   Size:        275    1696
 
NspI r_CATG'y

Cuts at:      0     732    1431    1524    1665    1971 
   Size:        732     699      93     141     306

  Fragments arranged by size: 

                 732     699     306     141      93
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     783    1175    1971 
   Size:        783     392     796

  Fragments arranged by size: 

                 796     783     392
 
PfoI T'CCnGG_A

Cuts at:      0    1511    1971 
   Size:       1511     460
 
PmlI CAC'GTG

Cuts at:      0     933    1971 
   Size:        933    1038
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     685     717    1971 
   Size:        685      32    1254

  Fragments arranged by size: 

                1254     685      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     671    1971 
   Size:        671    1300
 
PshAI GACnn'nnGTC

Cuts at:      0     107    1971 
   Size:        107    1864
 
PstI C_TGCA'G

Cuts at:      0    1637    1971 
   Size:       1637     334
 
PvuII CAG'CTG

Cuts at:      0    1854    1930    1971 
   Size:       1854      76      41

  Fragments arranged by size: 

                1854      76      41
 
SacI G_AGCT'C

Cuts at:      0     362    1971 
   Size:        362    1609
 
ScaI AGT'ACT

Cuts at:      0    1372    1971 
   Size:       1372     599
 
SfcI C'TryA_G

Cuts at:      0     954    1633    1971 
   Size:        954     679     338

  Fragments arranged by size: 

                 954     679     338
 
SfoI GGC'GCC

Cuts at:      0    1093    1180    1971 
   Size:       1093      87     791

  Fragments arranged by size: 

                1093     791      87
 
SmlI C'TyrA_G

Cuts at:      0     423    1971 
   Size:        423    1548
 
SphI G_CATG'C

Cuts at:      0     732    1665    1971 
   Size:        732     933     306

  Fragments arranged by size: 

                 933     732     306
 
StyI C'CwwG_G

Cuts at:      0     782    1971 
   Size:        782    1189
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1849    1971 
   Size:       1849     122
 
TatI w'GTAC_w

Cuts at:      0    1370    1424    1496    1971 
   Size:       1370      54      72     475

  Fragments arranged by size: 

                1370     475      72      54
 
ZraI GAC'GTC

Cuts at:      0      64     225    1971 
   Size:         64     161    1746

  Fragments arranged by size: 

                1746     161      64
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     AleI     AlfI    AlwNI    ApaLI     ApoI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BclI
     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII     DrdI
     EaeI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I
     KasI     MfeI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI
    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI     PstI    PvuII
     SacI     ScaI     SfcI     SfoI     SmlI     SphI     StyI    TaqII
     TatI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AloI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI     BcgI
    BciVI    BfrBI     BglI    BglII     BmtI     BplI    BsaBI    BsiEI
    BsiWI    BsmBI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I
    BtgZI     ClaI    CspCI     DraI     EagI     EarI     EciI    EcoRI
    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI
     MluI     MmeI     MscI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PmeI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com